Mouse Multiclonal CD8 alpha antibody. Carrier free. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human CD8A.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [C8/468 + C8/144B] - BSA and Azide free (AB213020), expandable thumbnail](https://content.abcam.com/products/images/cd8-alpha-antibody-c8-468-c8-144b-bsa-and-azide-free-ab213020--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img100770.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [C8/468 + C8/144B] - BSA and Azide free (AB213020)")
Publications
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- Nature cancer 2:545-5622021Neutrophil oxidative stress mediates obesity-associated vascular dysfunction and metastatic transmigration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSheri A C McDowell,Robin B E Luo,Azadeh Arabzadeh,Samuel Doré,Nicolas C Bennett,Valérie Breton,Elham Karimi,Morteza Rezanejad,Ryan R Yang,Katherine D Lach,Marianne S M Issac,Bozena Samborska,Lucas J M Perus,Dan Moldoveanu,Yuhong Wei,Benoit Fiset,Roni F Rayes,Ian R Watson,Lawrence Kazak,Marie-Christine Guiot,Pierre O Fiset,Jonathan D Spicer,Andrew J Dannenberg,Logan A Walsh,Daniela F QuailPubMed 35122017
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Multiclonal
Immunogen
- Synthetic Peptide within Human CD8A. The exact immunogen used to generate this antibody is proprietary information. Database link P01732
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.50000-1.00000 µg/mL | Notes (for 30 minutes at RT) Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Associated Products
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1 product for Alternative Version
Target data
Function
Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs). This mechanism enables CTLs to recognize and eliminate infected cells and tumor cells. In NK-cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing conjugation and lysis of multiple target cells. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells.
Alternative names
CD8a, MAL, CD8A, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2
Recommended products
Mouse Multiclonal CD8 alpha antibody. Carrier free. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human CD8A.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Multiclonal
- Immunogens
- Synthetic Peptide within Human CD8A. The exact immunogen used to generate this antibody is proprietary information. Database link P01732
- Carrier free
- Yes
- Clone number
- C8/468 + C8/144B
- Purification technique
- Affinity purification Protein A/G
- Light chain type
- kappa
- Concentration
- Purification notes
ab213020 is purified from Bioreactor Concentrate by Protein A/G.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Please note that this antibody is an oligoclonal antibody. It is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD8 alpha also known as CD8A or CD8 protein is a glycoprotein subunit expressed on the surface of cytotoxic T lymphocytes. It has a mass of approximately 32 kDa. Found on the surface cell membrane CD8 alpha functions primarily in the immune response specifically in the recognition of antigens bound to major histocompatibility complex (MHC) class I molecules. Often scientists use CD8 antibodies for detection and CD8 IHC or immunohistochemistry for localization studies.
- Biological function summary
The CD8 alpha protein plays a critical role in T-cell mediated immune responses. It forms a heterodimer with the CD8 beta chain creating the CD8 alpha-beta complex that strengthens T-cell interaction with antigen-presenting cells. CD8 alpha also helps in signaling processes that activate T cells equipping them to destroy infected or malignant cells. Researchers often study CD8 alpha peptides to understand its interactions better.
- Pathways
CD8 alpha is integral to the T-cell receptor signaling pathway and the cytotoxic T lymphocyte (CTL) pathway. The T-cell receptor complex which includes the CD8 molecule transmits signals that are important for T-cell activation and function. CD8 interacts with key proteins such as the T-cell receptor (TCR) and MHC class I molecules facilitating targeted responses against pathogens. These pathways highlight CD8 alpha’s role in adaptive immunity.
- Associated diseases and disorders
CD8 alpha is most prominently associated with viral infections and cancer. Conditions like HIV and some forms of leukemia show altered CD8 function highlighting the protein's role in immune surveillance. In HIV infection for instance CD8 T cells reduce in number impairing the immune response. CD8 alpha’s connection to the immune system places it alongside other immune proteins such as CD4 and MHC molecules in the context of immune dysfunction.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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1 product image
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [C8/468 + C8/144B] - BSA and Azide free (ab213020), expandable thumbnail](https://content.abcam.com/products/images/cd8-alpha-antibody-c8-468-c8-144b-bsa-and-azide-free-ab213020--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img100770.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [C8/468 + C8/144B] - BSA and Azide free (ab213020)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [C8/468 + C8/144B] - BSA and Azide free (ab213020)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue labeling CD8 alpha with ab213020 at 1 μg/ml dilution.
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