Rabbit Recombinant Monoclonal CD8 alpha antibody. Carrier free. Suitable for IP, Flow Cyt, ICC/IF, IHC-P, mIHC and reacts with Human, Rat samples.
Images
![Immunoprecipitation - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (AB251596), expandable thumbnail](https://content.abcam.com/products/images/cd8-alpha-antibody-cal66-bsa-and-azide-free-ab251596--immunoprecipitation-img4297.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (AB251596)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (AB251596), expandable thumbnail](https://content.abcam.com/products/images/cd8-alpha-antibody-cal66-bsa-and-azide-free-ab251596--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img98636.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (AB251596)")
![Flow Cytometry - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (AB251596), expandable thumbnail](https://content.abcam.com/products/images/cd8-alpha-antibody-cal66-bsa-and-azide-free-ab251596--flow-cytometry-img62984.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (AB251596)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (AB251596), expandable thumbnail](https://content.abcam.com/products/images/cd8-alpha-antibody-cal66-bsa-and-azide-free-ab251596--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img129704.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (AB251596)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (AB251596), expandable thumbnail](https://content.abcam.com/products/images/cd8-alpha-antibody-cal66-bsa-and-azide-free-ab251596--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img105830.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (AB251596)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Constituents: PBS
- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | ICC/IF | IHC-P | mIHC | |
|---|---|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Tested | Tested | Tested |
| Rat | Expected | Expected | Not recommended | Expected | Tested | Expected |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes Use at 1 μg/ml for rat Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Use at 1 μg/ml for rat Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
mIHC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
1 products for Alternative Product
4 products for Alternative Version
Target data
Function
Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs). This mechanism enables CTLs to recognize and eliminate infected cells and tumor cells. In NK-cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing conjugation and lysis of multiple target cells. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells.
Alternative names
T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2, CD8A, MAL
Recommended products
Rabbit Recombinant Monoclonal CD8 alpha antibody. Carrier free. Suitable for IP, Flow Cyt, ICC/IF, IHC-P, mIHC and reacts with Human, Rat samples.
Alternative names
T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2, CD8A, MAL
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Constituents: PBS
- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
CAL66
- Purification technique
Affinity purification Protein A
- Concentration
- Purification notes
Purity is greater than 99%.
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab251596 is the carrier-free version of Anti-CD8 alpha antibody [CAL66] ab237709.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD8 alpha also known as CD8A or CD8 protein is a glycoprotein subunit expressed on the surface of cytotoxic T lymphocytes. It has a mass of approximately 32 kDa. Found on the surface cell membrane CD8 alpha functions primarily in the immune response specifically in the recognition of antigens bound to major histocompatibility complex (MHC) class I molecules. Often scientists use CD8 antibodies for detection and CD8 IHC or immunohistochemistry for localization studies.
- Biological function summary
The CD8 alpha protein plays a critical role in T-cell mediated immune responses. It forms a heterodimer with the CD8 beta chain creating the CD8 alpha-beta complex that strengthens T-cell interaction with antigen-presenting cells. CD8 alpha also helps in signaling processes that activate T cells equipping them to destroy infected or malignant cells. Researchers often study CD8 alpha peptides to understand its interactions better.
- Pathways
CD8 alpha is integral to the T-cell receptor signaling pathway and the cytotoxic T lymphocyte (CTL) pathway. The T-cell receptor complex which includes the CD8 molecule transmits signals that are important for T-cell activation and function. CD8 interacts with key proteins such as the T-cell receptor (TCR) and MHC class I molecules facilitating targeted responses against pathogens. These pathways highlight CD8 alpha’s role in adaptive immunity.
- Associated diseases and disorders
CD8 alpha is most prominently associated with viral infections and cancer. Conditions like HIV and some forms of leukemia show altered CD8 function highlighting the protein's role in immune surveillance. In HIV infection for instance CD8 T cells reduce in number impairing the immune response. CD8 alpha’s connection to the immune system places it alongside other immune proteins such as CD4 and MHC molecules in the context of immune dysfunction.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
13 product images
![Immunoprecipitation - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (ab251596), expandable thumbnail](https://content.abcam.com/products/images/cd8-alpha-antibody-cal66-bsa-and-azide-free-ab251596--immunoprecipitation-img4297.jpg "Immunoprecipitation - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (ab251596)")
Immunoprecipitation - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (ab251596)
CD8 alpha was immunoprecipitated from 0.35 mg of human thymus lysate with Anti-CD8 alpha antibody [CAL66] ab237709 at 1/30 dilution. Western blot was performed from the immunoprecipitate using Anti-CD8 alpha antibody [CAL66] ab237709 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used as secondary antibody at 1/5000 dilution.
Lane 1: Human thymus lysate 10 μg (Input).
Lane 2: Anti-CD8 alpha antibody [CAL66] ab237709 IP in human thymus lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-CD8 alpha antibody [CAL66] ab237709 in human thymus lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 100 seconds.This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (Anti-CD8 alpha antibody [CAL66] ab237709).
All lanes: Immunoprecipitation - Anti-CD8 alpha antibody [CAL66] (Anti-CD8 alpha antibody [CAL66] ab237709)
Predicted band size: 26 kDa
Observed band size: 26 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (ab251596), expandable thumbnail](https://content.abcam.com/products/images/cd8-alpha-antibody-cal66-bsa-and-azide-free-ab251596--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img98636.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (ab251596)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (ab251596)
Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CD8 alpha with Anti-CD8 alpha antibody [CAL66] ab237709 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on the human tonsil is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (Anti-CD8 alpha antibody [CAL66] ab237709).
![Flow Cytometry - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (ab251596), expandable thumbnail](https://content.abcam.com/products/images/cd8-alpha-antibody-cal66-bsa-and-azide-free-ab251596--flow-cytometry-img62984.jpg "Flow Cytometry - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (ab251596)")
Flow Cytometry - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (ab251596)
Flow cytometric analysis of 2% paraformaldehyde-fixed, 0.1% Tween 20 permeacblized human PBMC (peripheral blood mononuclear cells) labeling CD8 alpha with Anti-CD8 alpha antibody [CAL66] ab237709 at 1/500 dilution (Right) compared with Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Left).
Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) preadsorbed (Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) preadsorbed ab150097), at 1/5000 dilution was used as the secondary antibody.
Cells were surface stained with anti-CD8 alpha conjugated to BV421.The fixed with 2% PFA for 10min followed by intracellularly stained with rabbit IgG (Left) or Anti-CD8 alpha antibody [CAL66] ab237709 (Right). They recognize same populations.
This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (Anti-CD8 alpha antibody [CAL66] ab237709).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (ab251596), expandable thumbnail](https://content.abcam.com/products/images/cd8-alpha-antibody-cal66-bsa-and-azide-free-ab251596--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img129704.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (ab251596)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (ab251596)
Immunohistochemical analysis of paraffin-embedded human colon tissue labeling CD8 alpha with Anti-CD8 alpha antibody [CAL66] ab237709 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on the T lymphocytes in human colon is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (Anti-CD8 alpha antibody [CAL66] ab237709).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (ab251596), expandable thumbnail](https://content.abcam.com/products/images/cd8-alpha-antibody-cal66-bsa-and-azide-free-ab251596--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img105830.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (ab251596)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (ab251596)
Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue labeling CD8 alpha with Anti-CD8 alpha antibody [CAL66] ab237709 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on the T lymphocytes in human gastric carcinoma is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (Anti-CD8 alpha antibody [CAL66] ab237709).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (ab251596), expandable thumbnail](https://content.abcam.com/products/images/cd8-alpha-antibody-cal66-bsa-and-azide-free-ab251596--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img7662.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (ab251596)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (ab251596)
Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling CD8 alpha with Anti-CD8 alpha antibody [CAL66] ab237709 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on the rat spleen is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (Anti-CD8 alpha antibody [CAL66] ab237709).
![Immunocytochemistry/ Immunofluorescence - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (ab251596), expandable thumbnail](https://content.abcam.com/products/images/cd8-alpha-antibody-cal66-bsa-and-azide-free-ab251596--immunocytochemistry-immunofluorescence-img17291.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (ab251596)")
Immunocytochemistry/ Immunofluorescence - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (ab251596)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized human PBMC (peripheral blood mononuclear cells) labeling CD8 alpha with Anti-CD8 alpha antibody [CAL66] ab237709 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in subsets of human PBMC. The nuclear counter stain is DAPI (blue). CD3 is detected with an anti-CD3 antibody-Alexa Fluor® 647 comjugate at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (Anti-CD8 alpha antibody [CAL66] ab237709).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (ab251596), expandable thumbnail](https://content.abcam.com/products/images/cd8-alpha-antibody-cal66-bsa-and-azide-free-ab251596--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img100132.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (ab251596)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (ab251596)
Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD8 alpha using Anti-CD8 alpha antibody [CAL66] ab237709 at 0.25 μg/ml in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (Anti-CD8 alpha antibody [CAL66] ab237709).
![Multiplex immunohistochemistry - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (ab251596), expandable thumbnail](https://content.abcam.com/products/images/cd8-alpha-antibody-cal66-bsa-and-azide-free-ab251596--multiplex-immunohistochemistry-img401808.jpg "Multiplex immunohistochemistry - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (ab251596)")
This image is courtesy of ImmunoAtlas.Multiplex immunohistochemistry - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (ab251596)
Fluorescence multiplex immunohistochemical analysis of Human breast cancer tissue (formalin-fixed paraffin-embedded section).
Merged staining of Anti-PD-L1 (Anti-PD-L1 antibody [CAL10] - BSA and Azide free ab251611; cyan; Opal™ 520), Anti-Granzyme B (Anti-Granzyme B antibody [EPR20129-217] - BSA and Azide free ab219803; yellow; Opal™ 540), Anti-PD1 (Anti-PD1 antibody [CAL20] - BSA and Azide free ab251613; magenta; Opal™ 570), Anti-pan Cytokeratin (Anti-pan Cytokeratin antibody [C-11] - BSA and Azide free ab264485; red; Opal™ 620), Anti-EpCAM (Anti-EpCAM antibody [EPR20532-225] - BSA and Azide free ab225894; red; Opal™ 620), Anti-CD8 alpha (ab251596; green; Opal™ 650) and Anti-FOXP3 (Anti-FOXP3 antibody [236A/E7] - BSA and Azide free ab96048; orange; Opal™ 690). EpCAM and pan-cytokeratin share the same dye and color. Dyes are pseudo-colored for better contrast of the markers.
The immunostaining was performed on a Leica Biosystems BOND® MAX instrument with an Opal™ 6-Plex Detection Kit (NEL821001KT, Akoya Biosciences®).
The section was incubated in six rounds of staining; sequentially for Anti-PD-L1 antibody [CAL10] - BSA and Azide free ab251611 (1/750 dilution), Anti-Granzyme B antibody [EPR20129-217] - BSA and Azide free ab219803 (1/250 dilution), Anti-PD1 antibody [CAL20] - BSA and Azide free ab251613 (1/750 dilution), Anti-pan Cytokeratin antibody [C-11] - BSA and Azide free ab264485 (0.5 μg/ml), Anti-EpCAM antibody [EPR20532-225] - BSA and Azide free ab225894 (1/1250 dilution), ab251596 (1/1500 dilution) and Anti-FOXP3 antibody [236A/E7] - BSA and Azide free ab96048 (10 μg/ml); each using a separate fluorescent tyramide signal amplification system. EDTA based antigen retrieval (Leica Biosystems BOND® Epitope Retrieval Solution 2, pH 9.0, 20 minutes) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity. DAPI (dark blue) was used as a nuclear counter stain.
Microscopy and pseudocoloring of individual Opal™ dyes was performed using a Vectra 3 Imaging System (Akoya Biosciences®).This data is courtesy of ImmunoAtlas and it can be found here.
This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (Anti-CD8 alpha antibody [CAL66] ab237709).![Multiplex immunohistochemistry - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (ab251596), expandable thumbnail](https://content.abcam.com/products/images/cd8-alpha-antibody-cal66-bsa-and-azide-free-ab251596--multiplex-immunohistochemistry-img401812.jpg "Multiplex immunohistochemistry - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (ab251596)")
This image is courtesy of ImmunoAtlas.Multiplex immunohistochemistry - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (ab251596)
Fluorescence multiplex immunohistochemical analysis of Human breast cancer tissue (formalin-fixed paraffin-embedded section).
Merged staining of Anti-PD-L1 (Anti-PD-L1 antibody [CAL10] - BSA and Azide free ab251611; cyan; Opal™ 520), Anti-Granzyme B (Anti-Granzyme B antibody [EPR20129-217] - BSA and Azide free ab219803; yellow; Opal™ 540), Anti-PD1 (Anti-PD1 antibody [CAL20] - BSA and Azide free ab251613; magenta; Opal™ 570), Anti-pan Cytokeratin (Anti-pan Cytokeratin antibody [C-11] - BSA and Azide free ab264485; red; Opal™ 620), Anti-EpCAM (Anti-EpCAM antibody [EPR20532-225] - BSA and Azide free ab225894; red; Opal™ 620), Anti-CD8 alpha (ab251596; green; Opal™ 650) and Anti-FOXP3 (Anti-FOXP3 antibody [236A/E7] - BSA and Azide free ab96048; orange; Opal™ 690). EpCAM and pan-cytokeratin share the same dye and color. Dyes are pseudo-colored for better contrast of the markers.
The immunostaining was performed on a Leica Biosystems BOND® MAX instrument with an Opal™ 6-Plex Detection Kit (NEL821001KT, Akoya Biosciences®).
The section was incubated in six rounds of staining; sequentially for Anti-PD-L1 antibody [CAL10] - BSA and Azide free ab251611 (1/750 dilution), Anti-Granzyme B antibody [EPR20129-217] - BSA and Azide free ab219803 (1/250 dilution), Anti-PD1 antibody [CAL20] - BSA and Azide free ab251613 (1/750 dilution), Anti-pan Cytokeratin antibody [C-11] - BSA and Azide free ab264485 (0.5 μg/ml), Anti-EpCAM antibody [EPR20532-225] - BSA and Azide free ab225894 (1/1250 dilution), ab251596 (1/1500 dilution) and Anti-FOXP3 antibody [236A/E7] - BSA and Azide free ab96048 (10 μg/ml); each using a separate fluorescent tyramide signal amplification system. EDTA based antigen retrieval (Leica Biosystems BOND® Epitope Retrieval Solution 2, pH 9.0, 20 minutes) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity. DAPI (dark blue) was used as a nuclear counter stain.
Microscopy and pseudocoloring of individual Opal™ dyes was performed using a Vectra 3 Imaging System (Akoya Biosciences®).This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (Anti-CD8 alpha antibody [CAL66] ab237709).
This data is courtesy of ImmunoAtlas and it can be found here.
![Multiplex immunohistochemistry - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (ab251596), expandable thumbnail](https://content.abcam.com/products/images/cd8-alpha-antibody-cal66-bsa-and-azide-free-ab251596--multiplex-immunohistochemistry-img401809.jpg "Multiplex immunohistochemistry - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (ab251596)")
This image is courtesy of ImmunoAtlas.Multiplex immunohistochemistry - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (ab251596)
Fluorescence multiplex immunohistochemical analysis of Human breast cancer tissue (formalin-fixed paraffin-embedded section).
Merged staining of Anti-PD-L1 (Anti-PD-L1 antibody [CAL10] - BSA and Azide free ab251611; cyan; Opal™ 520), Anti-Granzyme B (Anti-Granzyme B antibody [EPR20129-217] - BSA and Azide free ab219803; yellow; Opal™ 540), Anti-PD1 (Anti-PD1 antibody [CAL20] - BSA and Azide free ab251613; magenta; Opal™ 570), Anti-pan Cytokeratin (Anti-pan Cytokeratin antibody [C-11] - BSA and Azide free ab264485; red; Opal™ 620), Anti-EpCAM (Anti-EpCAM antibody [EPR20532-225] - BSA and Azide free ab225894; red; Opal™ 620), Anti-CD8 alpha (ab251596; green; Opal™ 650) and Anti-FOXP3 (Anti-FOXP3 antibody [236A/E7] - BSA and Azide free ab96048; orange; Opal™ 690). EpCAM and pan-cytokeratin share the same dye and color. Dyes are pseudo-colored for better contrast of the markers.
The immunostaining was performed on a Leica Biosystems BOND® MAX instrument with an Opal™ 6-Plex Detection Kit (NEL821001KT, Akoya Biosciences®).
The section was incubated in six rounds of staining; sequentially for Anti-PD-L1 antibody [CAL10] - BSA and Azide free ab251611 (1/750 dilution), Anti-Granzyme B antibody [EPR20129-217] - BSA and Azide free ab219803 (1/250 dilution), Anti-PD1 antibody [CAL20] - BSA and Azide free ab251613 (1/750 dilution), Anti-pan Cytokeratin antibody [C-11] - BSA and Azide free ab264485 (0.5 μg/ml), Anti-EpCAM antibody [EPR20532-225] - BSA and Azide free ab225894 (1/1250 dilution), ab251596 (1/1500 dilution) and Anti-FOXP3 antibody [236A/E7] - BSA and Azide free ab96048 (10 μg/ml); each using a separate fluorescent tyramide signal amplification system. EDTA based antigen retrieval (Leica Biosystems BOND® Epitope Retrieval Solution 2, pH 9.0, 20 minutes) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity. DAPI (dark blue) was used as a nuclear counter stain.
Microscopy and pseudocoloring of individual Opal™ dyes was performed using a Vectra 3 Imaging System (Akoya Biosciences®).This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (Anti-CD8 alpha antibody [CAL66] ab237709).
This data is courtesy of ImmunoAtlas and it can be found here.
![Multiplex immunohistochemistry - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (ab251596), expandable thumbnail](https://content.abcam.com/products/images/cd8-alpha-antibody-cal66-bsa-and-azide-free-ab251596--multiplex-immunohistochemistry-img401811.jpg "Multiplex immunohistochemistry - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (ab251596)")
This image is courtesy of ImmunoAtlas.Multiplex immunohistochemistry - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (ab251596)
Fluorescence multiplex immunohistochemical analysis of Human breast cancer tissue (formalin-fixed paraffin-embedded section).
Merged staining of Anti-PD-L1 (Anti-PD-L1 antibody [CAL10] - BSA and Azide free ab251611; cyan; Opal™ 520), Anti-Granzyme B (Anti-Granzyme B antibody [EPR20129-217] - BSA and Azide free ab219803; yellow; Opal™ 540), Anti-PD1 (Anti-PD1 antibody [CAL20] - BSA and Azide free ab251613; magenta; Opal™ 570), Anti-pan Cytokeratin (Anti-pan Cytokeratin antibody [C-11] - BSA and Azide free ab264485; red; Opal™ 620), Anti-EpCAM (Anti-EpCAM antibody [EPR20532-225] - BSA and Azide free ab225894; red; Opal™ 620), Anti-CD8 alpha (ab251596; green; Opal™ 650) and Anti-FOXP3 (Anti-FOXP3 antibody [236A/E7] - BSA and Azide free ab96048; orange; Opal™ 690). EpCAM and pan-cytokeratin share the same dye and color. Dyes are pseudo-colored for better contrast of the markers.
The immunostaining was performed on a Leica Biosystems BOND® MAX instrument with an Opal™ 6-Plex Detection Kit (NEL821001KT, Akoya Biosciences®).
The section was incubated in six rounds of staining; sequentially for Anti-PD-L1 antibody [CAL10] - BSA and Azide free ab251611 (1/750 dilution), Anti-Granzyme B antibody [EPR20129-217] - BSA and Azide free ab219803 (1/250 dilution), Anti-PD1 antibody [CAL20] - BSA and Azide free ab251613 (1/750 dilution), Anti-pan Cytokeratin antibody [C-11] - BSA and Azide free ab264485 (0.5 μg/ml), Anti-EpCAM antibody [EPR20532-225] - BSA and Azide free ab225894 (1/1250 dilution), ab251596 (1/1500 dilution) and Anti-FOXP3 antibody [236A/E7] - BSA and Azide free ab96048 (10 μg/ml); each using a separate fluorescent tyramide signal amplification system. EDTA based antigen retrieval (Leica Biosystems BOND® Epitope Retrieval Solution 2, pH 9.0, 20 minutes) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity. DAPI (dark blue) was used as a nuclear counter stain.
Microscopy and pseudocoloring of individual Opal™ dyes was performed using a Vectra 3 Imaging System (Akoya Biosciences®).This data is courtesy of ImmunoAtlas and it can be found here.
This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (Anti-CD8 alpha antibody [CAL66] ab237709).
![Multiplex immunohistochemistry - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (ab251596), expandable thumbnail](https://content.abcam.com/products/images/cd8-alpha-antibody-cal66-bsa-and-azide-free-ab251596--multiplex-immunohistochemistry-img401810.jpg "Multiplex immunohistochemistry - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (ab251596)")
This image is courtesy of ImmunoAtlas.Multiplex immunohistochemistry - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (ab251596)
Fluorescence multiplex immunohistochemical analysis of Human breast cancer tissue (formalin-fixed paraffin-embedded section).
Merged staining of Anti-PD-L1 (Anti-PD-L1 antibody [CAL10] - BSA and Azide free ab251611; cyan; Opal™ 520), Anti-Granzyme B (Anti-Granzyme B antibody [EPR20129-217] - BSA and Azide free ab219803; yellow; Opal™ 540), Anti-PD1 (Anti-PD1 antibody [CAL20] - BSA and Azide free ab251613; magenta; Opal™ 570), Anti-pan Cytokeratin (Anti-pan Cytokeratin antibody [C-11] - BSA and Azide free ab264485; red; Opal™ 620), Anti-EpCAM (Anti-EpCAM antibody [EPR20532-225] - BSA and Azide free ab225894; red; Opal™ 620), Anti-CD8 alpha (ab251596; green; Opal™ 650) and Anti-FOXP3 (Anti-FOXP3 antibody [236A/E7] - BSA and Azide free ab96048; orange; Opal™ 690). EpCAM and pan-cytokeratin share the same dye and color. Dyes are pseudo-colored for better contrast of the markers.
The immunostaining was performed on a Leica Biosystems BOND® MAX instrument with an Opal™ 6-Plex Detection Kit (NEL821001KT, Akoya Biosciences®).
The section was incubated in six rounds of staining; sequentially for Anti-PD-L1 antibody [CAL10] - BSA and Azide free ab251611 (1/750 dilution), Anti-Granzyme B antibody [EPR20129-217] - BSA and Azide free ab219803 (1/250 dilution), Anti-PD1 antibody [CAL20] - BSA and Azide free ab251613 (1/750 dilution), Anti-pan Cytokeratin antibody [C-11] - BSA and Azide free ab264485 (0.5 μg/ml), Anti-EpCAM antibody [EPR20532-225] - BSA and Azide free ab225894 (1/1250 dilution), ab251596 (1/1500 dilution) and Anti-FOXP3 antibody [236A/E7] - BSA and Azide free ab96048 (10 μg/ml); each using a separate fluorescent tyramide signal amplification system. EDTA based antigen retrieval (Leica Biosystems BOND® Epitope Retrieval Solution 2, pH 9.0, 20 minutes) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity. DAPI (dark blue) was used as a nuclear counter stain.
Microscopy and pseudocoloring of individual Opal™ dyes was performed using a Vectra 3 Imaging System (Akoya Biosciences®).This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (Anti-CD8 alpha antibody [CAL66] ab237709).
This data is courtesy of ImmunoAtlas and it can be found here.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (ab251596), expandable thumbnail](https://content.abcam.com/products/images/cd8-alpha-antibody-cal66-bsa-and-azide-free-ab251596--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img7662.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (ab251596)")
![Immunocytochemistry/ Immunofluorescence - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (ab251596), expandable thumbnail](https://content.abcam.com/products/images/cd8-alpha-antibody-cal66-bsa-and-azide-free-ab251596--immunocytochemistry-immunofluorescence-img17291.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (ab251596)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (ab251596), expandable thumbnail](https://content.abcam.com/products/images/cd8-alpha-antibody-cal66-bsa-and-azide-free-ab251596--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img100132.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (ab251596)")
![Multiplex immunohistochemistry - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (ab251596), expandable thumbnail](https://content.abcam.com/products/images/cd8-alpha-antibody-cal66-bsa-and-azide-free-ab251596--multiplex-immunohistochemistry-img401808.jpg/_jcr_content/renditions/webp-475.webp "Multiplex immunohistochemistry - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (ab251596)")
![Multiplex immunohistochemistry - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (ab251596), expandable thumbnail](https://content.abcam.com/products/images/cd8-alpha-antibody-cal66-bsa-and-azide-free-ab251596--multiplex-immunohistochemistry-img401812.jpg/_jcr_content/renditions/webp-475.webp "Multiplex immunohistochemistry - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (ab251596)")
![Multiplex immunohistochemistry - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (ab251596), expandable thumbnail](https://content.abcam.com/products/images/cd8-alpha-antibody-cal66-bsa-and-azide-free-ab251596--multiplex-immunohistochemistry-img401809.jpg/_jcr_content/renditions/webp-475.webp "Multiplex immunohistochemistry - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (ab251596)")
![Multiplex immunohistochemistry - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (ab251596), expandable thumbnail](https://content.abcam.com/products/images/cd8-alpha-antibody-cal66-bsa-and-azide-free-ab251596--multiplex-immunohistochemistry-img401811.jpg/_jcr_content/renditions/webp-475.webp "Multiplex immunohistochemistry - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (ab251596)")
![Multiplex immunohistochemistry - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (ab251596), expandable thumbnail](https://content.abcam.com/products/images/cd8-alpha-antibody-cal66-bsa-and-azide-free-ab251596--multiplex-immunohistochemistry-img401810.jpg/_jcr_content/renditions/webp-475.webp "Multiplex immunohistochemistry - Anti-CD8 alpha antibody [CAL66] - BSA and Azide free (ab251596)")
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com