AB251597

Anti-CD8 alpha antibody [CAL67] - BSA and Azide free

RabMAb
Recombinant
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(3 Publications)

Rabbit Recombinant Monoclonal CD8 alpha antibody. Carrier free. Suitable for IHC-P and reacts with Human samples. Cited in 3 publications.

View Alternative Names

CD8a, MAL, CD8A, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [CAL67] - BSA and Azide free (AB251597)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [CAL67] - BSA and Azide free (AB251597)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CD8 alpha with ab237710 at 1/1500 dilution (0.352 μg/ml), followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on human tonsil tissue is observed. Counterstained with hematoxylin. Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab237710).

Immunocytochemistry/ Immunofluorescence - Anti-CD8 alpha antibody [CAL67] - BSA and Azide free (AB251597)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-CD8 alpha antibody [CAL67] - BSA and Azide free (AB251597)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized human PBMC (human primary peripheral blood mononuclear cell) cells labeling CD8 alpha with ab237710 at 1/100 dilution (5.3μg), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in subsets of human PBMC. The nuclear counter stain is DAPI (blue). Counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) at a 1/200 dilution (red). The negative control is the secondary antibody only.

This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab237710).

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [CAL67] - BSA and Azide free (AB251597)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling CD8 alpha with ab237710 at 1/1500 dilution (0.352 μg/ml), followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on the T lymphocytes in the human colon is observed. Counterstained with hematoxylin. Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab237710).

Unconjugated

Anti-CD8 alpha antibody [CAL67]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-CD8 alpha antibody [CAL67]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-CD8 alpha antibody [CAL67]
660 APC

APC Anti-CD8 alpha antibody [CAL67]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-CD8 alpha antibody [CAL67]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-CD8 alpha antibody [CAL67]
578 PE

PE Anti-CD8 alpha antibody [CAL67]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-CD8 alpha antibody [CAL67]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

CAL67

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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Product details

ab251597 is the carrier-free version of ab237710.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Purity is greater than 99%.

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD8 alpha also known as CD8A or CD8 protein is a glycoprotein subunit expressed on the surface of cytotoxic T lymphocytes. It has a mass of approximately 32 kDa. Found on the surface cell membrane CD8 alpha functions primarily in the immune response specifically in the recognition of antigens bound to major histocompatibility complex (MHC) class I molecules. Often scientists use CD8 antibodies for detection and CD8 IHC or immunohistochemistry for localization studies.

Biological function summary

The CD8 alpha protein plays a critical role in T-cell mediated immune responses. It forms a heterodimer with the CD8 beta chain creating the CD8 alpha-beta complex that strengthens T-cell interaction with antigen-presenting cells. CD8 alpha also helps in signaling processes that activate T cells equipping them to destroy infected or malignant cells. Researchers often study CD8 alpha peptides to understand its interactions better.

Pathways

CD8 alpha is integral to the T-cell receptor signaling pathway and the cytotoxic T lymphocyte (CTL) pathway. The T-cell receptor complex which includes the CD8 molecule transmits signals that are important for T-cell activation and function. CD8 interacts with key proteins such as the T-cell receptor (TCR) and MHC class I molecules facilitating targeted responses against pathogens. These pathways highlight CD8 alpha’s role in adaptive immunity.

CD8 alpha is most prominently associated with viral infections and cancer. Conditions like HIV and some forms of leukemia show altered CD8 function highlighting the protein's role in immune surveillance. In HIV infection for instance CD8 T cells reduce in number impairing the immune response. CD8 alpha’s connection to the immune system places it alongside other immune proteins such as CD4 and MHC molecules in the context of immune dysfunction.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The protein expressed by the gene CD8A is an integral membrane glycoprotein crucial for immune responses, operating mainly in T-cells as a coreceptor for MHC class I molecule : peptide complexes. These peptides originate from cytosolic proteins, unlike class II peptides, which are from extracellular proteins. CD8A interacts with the T-cell receptor (TCR) and MHC class I proteins on antigen-presenting cells, recruiting the Src kinase LCK to the TCR-CD3 complex. LCK phosphorylates substrates, triggering signaling pathways that lead to the production of lymphokines, enhanced motility, adhesion, and activation of cytotoxic T-lymphocytes (CTLs), thereby aiding in the recognition and elimination of infected or tumor cells. Additionally, in natural killer (NK) cells, CD8A homodimers on the cell surface provide a survival mechanism for conjugating with and lysing multiple target cells. CD8A homodimers also facilitate the survival and differentiation of activated lymphocytes into memory CD8 T-cells. This supplementary information is collated from multiple sources and compiled automatically.

See full target information CD8A

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 15:878 PubMed39632825

2024

Colorectal cancer patients-derived immunity-organoid platform unveils cancer-specific tissue markers associated with immunotherapy resistance.

Applications

Unspecified application

Species

Unspecified reactive species

A Esposito,A Agostini,G Quero,G Piro,L Priori,A Caggiano,G Scaglione,A Battaglia,M A Calegari,L Salvatore,M Bensi,M G Maratta,A Ceccarelli,G Trovato,G Genovese,E Gurreri,S Ascrizzi,M Martini,C Fiorillo,A Fattorossi,F De Sanctis,S Ugel,V Corbo,S Alfieri,G Tortora,C Carbone

Journal of nanobiotechnology 22:667 PubMed39472933

2024

Losartan-based nanocomposite hydrogel overcomes chemo-immunotherapy resistance by remodeling tumor mechanical microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaodong Hou,Yuting Shen,Bin Huang,Qiuyan Li,Shaoyue Li,Tingting Jiang,Xuexia Shan,Weichen Xu,Shuo Liu,Shengbo Wu,De Zhao,Anqi Zhu,Liping Sun,Huixiong Xu,Wenwen Yue

Journal of translational medicine 21:843 PubMed37996891

2023

Talniflumate abrogates mucin immune suppressive barrier improving efficacy of gemcitabine and nab-paclitaxel treatment in pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Antonio Agostini,Ilaria Guerriero,Geny Piro,Giuseppe Quero,Luca Roberto,Annachiara Esposito,Alessia Caggiano,Lorenzo Priori,Giulia Scaglione,Francesco De Sanctis,Antonella Sistigu,Martina Musella,Alberto Larghi,Gianenrico Rizzatti,Donatella Lucchetti,Sergio Alfieri,Alessandro Sgambato,Emilio Bria,Laura Bizzozero,Sabrina Arena,Stefano Ugel,Vincenzo Corbo,Giampaolo Tortora,Carmine Carbone

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD8 alpha antibody [CAL67] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [CAL67] - BSA and Azide free (AB251597)

Immunocytochemistry/ Immunofluorescence - Anti-CD8 alpha antibody [CAL67] - BSA and Azide free (AB251597)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [CAL67] - BSA and Azide free (AB251597)

Related conjugates and formulations (8)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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