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Rabbit Recombinant Monoclonal CD8 alpha antibody. Suitable for mIHC, IP, Flow Cyt, IHC-P and reacts with Human samples. Cited in 7 publications.


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [EPR22483-288] (AB245118), expandable thumbnail
  • Flow Cytometry - Anti-CD8 alpha antibody [EPR22483-288] (AB245118), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [EPR22483-288] (AB245118), expandable thumbnail
  • Immunoprecipitation - Anti-CD8 alpha antibody [EPR22483-288] (AB245118), expandable thumbnail
  • Multiplex immunohistochemistry - Anti-CD8 alpha antibody [EPR22483-288] (AB245118), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
mIHCIPFlow CytWBICC/IFIHC-P
Human
Tested
Tested
Tested
Not recommended
Not recommended
Tested

Tested
Tested

Species

Human

Dilution info

1/500

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

1/30

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

1/400

Notes

-

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs). This mechanism enables CTLs to recognize and eliminate infected cells and tumor cells. In NK-cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing conjugation and lysis of multiple target cells. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells.

Alternative names

MAL, CD8A, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2

Recommended products

Rabbit Recombinant Monoclonal CD8 alpha antibody. Suitable for mIHC, IP, Flow Cyt, IHC-P and reacts with Human samples. Cited in 7 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR22483-288

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CD8 alpha also known as CD8A or CD8 protein is a glycoprotein subunit expressed on the surface of cytotoxic T lymphocytes. It has a mass of approximately 32 kDa. Found on the surface cell membrane CD8 alpha functions primarily in the immune response specifically in the recognition of antigens bound to major histocompatibility complex (MHC) class I molecules. Often scientists use CD8 antibodies for detection and CD8 IHC or immunohistochemistry for localization studies.

Biological function summary

The CD8 alpha protein plays a critical role in T-cell mediated immune responses. It forms a heterodimer with the CD8 beta chain creating the CD8 alpha-beta complex that strengthens T-cell interaction with antigen-presenting cells. CD8 alpha also helps in signaling processes that activate T cells equipping them to destroy infected or malignant cells. Researchers often study CD8 alpha peptides to understand its interactions better.

Pathways

CD8 alpha is integral to the T-cell receptor signaling pathway and the cytotoxic T lymphocyte (CTL) pathway. The T-cell receptor complex which includes the CD8 molecule transmits signals that are important for T-cell activation and function. CD8 interacts with key proteins such as the T-cell receptor (TCR) and MHC class I molecules facilitating targeted responses against pathogens. These pathways highlight CD8 alpha’s role in adaptive immunity.

Associated diseases and disorders

CD8 alpha is most prominently associated with viral infections and cancer. Conditions like HIV and some forms of leukemia show altered CD8 function highlighting the protein's role in immune surveillance. In HIV infection for instance CD8 T cells reduce in number impairing the immune response. CD8 alpha’s connection to the immune system places it alongside other immune proteins such as CD4 and MHC molecules in the context of immune dysfunction.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [EPR22483-288] (ab245118), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [EPR22483-288] (ab245118)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CD8 alpha with ab245118 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on the T cell zone of human tonsil (PMID: 16504163) is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
    Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Flow Cytometry - Anti-CD8 alpha antibody [EPR22483-288] (ab245118), expandable thumbnail

    Flow Cytometry - Anti-CD8 alpha antibody [EPR22483-288] (ab245118)

    Flow cytometric analysis of human peripheral blood mononuclear cells (PBMC) labeling CD8 alpha with ab245118 at 1/400 (right panel) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (left panel). Goat anti rabbit IgG (Dylight® 488, ab98462) at 1/2000 dilution was used as the secondary antibody.

    Cells were stained with rabbit IgG (Left) or anti-CD8 alpha RabMab (Right). Then stained with Alexa Fluor® 647-conjugated anti-CD4. The expressions of CD4 and CD8 alpha are mutually exclusive.

    Gated on viable lymphocytes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [EPR22483-288] (ab245118), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [EPR22483-288] (ab245118)

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling CD8 alpha with ab245118 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on lymphocytes of human colon (PMID: 26310568) is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
    Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunoprecipitation - Anti-CD8 alpha antibody [EPR22483-288] (ab245118), expandable thumbnail

    Immunoprecipitation - Anti-CD8 alpha antibody [EPR22483-288] (ab245118)

    CD8 alpha was immunoprecipitated from 0.35 mg human thymus lysate with ab245118 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab245118 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/1000 dilution.

    Lane 1: Human thymus lysate 10 μg (Input).
    Lane 2: ab245118 IP in human thymus lysate.
    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab245118 in human thymus lysate.

    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 30 seconds.

    All lanes: Immunoprecipitation - Anti-CD8 alpha antibody [EPR22483-288] (ab245118)

    Predicted band size: 26 kDa

    Observed band size: 26 kDa

  • Multiplex immunohistochemistry - Anti-CD8 alpha antibody [EPR22483-288] (ab245118), expandable thumbnail

    Multiplex immunohistochemistry - Anti-CD8 alpha antibody [EPR22483-288] (ab245118)

    Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Human colon staining of PVRIG/CD112R with Anti-PVRIG/CD112R antibody [EPR29052-91] ab322348 at a 1/100 (5.16 µg/ml) dilution, CD19 with Anti-CD19 antibody [EPR28949-559] ab320735 at 1/2000 (0.255 µg/ml) dilution and CD8A with ab245118 at 1/1000 ( 0.375 µg/ml) dilution.

    Panel A: merged staining of anti-PVRIG/CD112R (green; Opal™520), anti-CD8A (magenta; Opal™570) and anti-CD19 (yellow; Opal™690) on Human colon.
    Panel B: anti-PVRIG/CD112R staining memory and effector CD8+ T lymphocytes in Human colon.
    Panel C: anti-CD8A staining CD8+ T lymphocytes in Human colon.
    Panel D: anti-CD19 staining B lymphocytes in Human colon.
    Nuclear DNA was labelled with DAPI (shown in blue). ).

    The section was incubated in three rounds of staining: in the order of Anti-PVRIG/CD112R antibody [EPR29052-91] ab322348, ab245118 and Anti-CD19 antibody [EPR28949-559] ab320735 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0 epitope retrieval solution2) for 20 mins.

  • Multiplex immunohistochemistry - Anti-CD8 alpha antibody [EPR22483-288] (ab245118), expandable thumbnail

    Multiplex immunohistochemistry - Anti-CD8 alpha antibody [EPR22483-288] (ab245118)

    Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Human tonsil tissue labeling CD8 alpha with ab245118 at 1/500 dilution, CD4 with Anti-CD4 antibody [SP35] - BSA and Azide free ab238798 at 1/500, and CD19 with Anti-CD19 antibody [SP291] - BSA and Azide free ab237772 at 1/5000 dilution.

    Panel A: merged staining of anti-CD8 alpha (magenta; Opal™690), anti-CD4 (green; Opal™520) and anti-CD19 (red; Opal™570) on human tonsil.
    Panel B: anti-CD8 alpha stained on cytotoxic T cells.
    Panel C: anti-CD4 stained on T helper cells.
    Panel D: anti-CD19 stained on B cells.

    Sections were treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins before antibody incubation. The section was incubated in three rounds of staining: in the order of ab245118 for 30 mins, then Anti-CD4 antibody [SP35] - BSA and Azide free ab238798 and Anti-CD19 antibody [SP291] - BSA and Azide free ab237772 for 10 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    DAPI was used as a nuclear counterstain.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

  • Multiplex immunohistochemistry - Anti-CD8 alpha antibody [EPR22483-288] (ab245118), expandable thumbnail

    Multiplex immunohistochemistry - Anti-CD8 alpha antibody [EPR22483-288] (ab245118)

    Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Human spleen staining of PVRIG/CD112R with Anti-PVRIG/CD112R antibody [EPR29052-91] ab322348 at a 1/100 (5.16 µg/ml) dilution, CD19 with Anti-CD19 antibody [EPR28949-559] ab320735 at 1/2000 (0.255 µg/ml) dilution and CD8A with ab245118 at 1/1000 ( 0.375 µg/ml) dilution.

    Panel A: merged staining of anti-PVRIG/CD112R (green; Opal™520), anti-CD8A (magenta; Opal™570) and anti-CD19 (yellow; Opal™690) on human spleen.
    Panel B: anti-PVRIG/CD112R staining memory and effector CD8+ T lymphocytes in human spleen.
    Panel C: anti-CD8A staining CD8+ T lymphocytes in human spleen.
    Panel D: anti-CD19 staining B lymphocytes in human spleen.
    Nuclear DNA was labelled with DAPI (shown in blue). ).

    The section was incubated in three rounds of staining: in the order of Anti-PVRIG/CD112R antibody [EPR29052-91] ab322348, ab245118 and Anti-CD19 antibody [EPR28949-559] ab320735 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0 epitope retrieval solution2) for 20 mins.

  • Multiplex immunohistochemistry - Anti-CD8 alpha antibody [EPR22483-288] (ab245118), expandable thumbnail

    Multiplex immunohistochemistry - Anti-CD8 alpha antibody [EPR22483-288] (ab245118)

    Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Human tonsil staining of PVRIG/CD112R with Anti-PVRIG/CD112R antibody [EPR29052-91] ab322348 at a 1/100 (5.16 µg/ml) dilution, CD19 with Anti-CD19 antibody [EPR28949-559] ab320735 at 1/2000 (0.255 µg/ml) dilution and CD8A with ab245118 at 1/1000 ( 0.375 µg/ml) dilution.

    Panel A: merged staining of anti-PVRIG/CD112R (green; Opal™520), anti-CD8A (magenta; Opal™570) and anti-CD19 (yellow; Opal™690) on human tonsil.
    Panel B: anti-PVRIG/CD112R staining memory and effector CD8+ T lymphocytes in human tonsil.
    Panel C: anti-CD8A staining CD8+ T lymphocytes in human tonsil.
    Panel D: anti-CD19 staining B lymphocytes in human tonsil.
    Nuclear DNA was labeled with DAPI (shown in blue). ).

    The section was incubated in three rounds of staining: in the order of Anti-PVRIG/CD112R antibody [EPR29052-91] ab322348, ab245118 and Anti-CD19 antibody [EPR28949-559] ab320735 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0 epitope retrieval solution2) for 20 mins.

  • Multiplex immunohistochemistry - Anti-CD8 alpha antibody [EPR22483-288] (ab245118), expandable thumbnail

    Multiplex immunohistochemistry - Anti-CD8 alpha antibody [EPR22483-288] (ab245118)

    Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Human spleen tissue labeling CD8 alpha with ab245118 at 1/500 dilution, CD4 with Anti-CD4 antibody [SP35] - BSA and Azide free ab238798 at 1/500, and CD19 with Anti-CD19 antibody [SP291] - BSA and Azide free ab237772 at 1/5000 dilution.

    Panel A: merged staining of anti-CD8 alpha (magenta; Opal™690), anti-CD4 (green; Opal™520) and anti-CD19 (red; Opal™570) on human spleen.
    Panel B: anti-CD8 alpha stained on cytotoxic T cells.
    Panel C: anti-CD4 stained on T helper cells.
    Panel D: anti-CD19 stained on B cells.

    Sections were treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins before antibody incubation. The section was incubated in three rounds of staining: in the order of ab245118 for 30 mins, then Anti-CD4 antibody [SP35] - BSA and Azide free ab238798 and Anti-CD19 antibody [SP291] - BSA and Azide free ab237772 for 10 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    DAPI was used as a nuclear counterstain.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

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