Anti-CD8 alpha antibody [SP239]

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [SP239] (AB178089)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CD8 alpha with ab178089 at followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human tonsil, performed on a Leica Biosystems BOND® RX instrument. Counterstained with Paraffin-embedded sections. Hematoxylin Secondary antibody only control : Used PBS instead of primary antibody.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [SP239] (AB178089)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human tonsil tissue labeling CD8 alpha with ab178089 at 1/100 dilution.

• mIHC

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Multiplex immunohistochemistry - Anti-CD8 alpha antibody [SP239] (AB178089)

Multichannel (Multiplex) immunohistochemistry analysis of Formalin/PFA-fixed paraffin-embedded sections of human tonsil tissue labeling NKG2D, NKG2a, and CD8 alpha. Panel A : merged staining of anti-CD8 alpha (red; Opal™690), anti-NKG2A (gray; Opal™520) and anti-NKG2D (green; Opal™570) on human tonsil. Panel B : anti-NKG2D (ab302907 at 1 : 2000 dilution (0.258 μg/ml)) signal on NK cells and T cell subsets. Panel C : anti-NKG2A (ab260035 at 1 : 2000 dilution (0.283 μg/ml)) signal on NK cells. Panel D : anti-CD8 alpha (ab178089 at 1 : 100 dilution (0.83 μg/ml)) signal on T cell subsets. Opal Polymer HRP Ms + Rb was used as secondary antibody. The section was incubated in three rounds of staining : in the order of ab178089, ab260035, and ab302907 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. Nuclear counterstain was DAPI. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-CD8 alpha antibody [SP239] (AB178089)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil labelling PD1 with ab243644 at 1/500 dilution (1.02 µg/mL) (D), CD8 with ab178089 at 1/100 dilution (0.83 μg/ml) (C) and TIM 3 with ab242080 at 1/500 dilution (2.09 μg/ml) (B). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.   Panel A : merged staining of anti-TIM 3 (green; Opal™690), anti-CD8 (red; Opal™520) and anti-PD1 (gray; Opal™570) on human tonsil. Panel B : anti-TIM 3 stained on membrane of a subset of immune cells. Panel C : anti-CD8 stained on membrane of a subset of T cells. Panel D : anti-PD1 stained on membrane of a subset of lymphocytes. The section was incubated in three rounds of staining : in the order of ab242080, ab243644 and ab178089 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [SP239] (AB178089)

Immunohistochemical analysis of formalin fixed paraffin embedded Human tonsil labelling CD8 with ab178089 at a concentration of 1 µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with a OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was performed with DISCOVERY cell conditioning solution (CC1) 100°C, pH8.5 for 32mins.

ab178089 anti DCD8 antibody SP239 was incubated for 16mins at 37°C. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

Customers are encouraged to optimise antigen retrieval conditions, antibody concentration, incubation times and temperature for best results in their own IHC assay workflow (automated and manual)