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Rabbit Recombinant Monoclonal CD8 alpha antibody. Suitable for IHC-P, mIHC and reacts with Human samples. Cited in 26 publications.


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [SP239] (AB178089), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [SP239] (AB178089), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [SP239] (AB178089), expandable thumbnail
  • Multiplex immunohistochemistry - Anti-CD8 alpha antibody [SP239] (AB178089), expandable thumbnail
  • Multiplex immunohistochemistry - Anti-CD8 alpha antibody [SP239] (AB178089), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PmIHC
Human
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/100

Notes

Antigen Retrieval: Boil tissue section in EDTA buffer, pH 8.0 for 10 min followed by cooling at room temperature for 20 min.

Tested
Tested

Species

Human

Dilution info

1/100

Notes

-

Associated Products

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Target data

Function

Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs). This mechanism enables CTLs to recognize and eliminate infected cells and tumor cells. In NK-cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing conjugation and lysis of multiple target cells. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells.

Alternative names

T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2, CD8A, MAL

Recommended products

Rabbit Recombinant Monoclonal CD8 alpha antibody. Suitable for IHC-P, mIHC and reacts with Human samples. Cited in 26 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

SP239

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CD8 alpha also known as CD8A or CD8 protein is a glycoprotein subunit expressed on the surface of cytotoxic T lymphocytes. It has a mass of approximately 32 kDa. Found on the surface cell membrane CD8 alpha functions primarily in the immune response specifically in the recognition of antigens bound to major histocompatibility complex (MHC) class I molecules. Often scientists use CD8 antibodies for detection and CD8 IHC or immunohistochemistry for localization studies.

Biological function summary

The CD8 alpha protein plays a critical role in T-cell mediated immune responses. It forms a heterodimer with the CD8 beta chain creating the CD8 alpha-beta complex that strengthens T-cell interaction with antigen-presenting cells. CD8 alpha also helps in signaling processes that activate T cells equipping them to destroy infected or malignant cells. Researchers often study CD8 alpha peptides to understand its interactions better.

Pathways

CD8 alpha is integral to the T-cell receptor signaling pathway and the cytotoxic T lymphocyte (CTL) pathway. The T-cell receptor complex which includes the CD8 molecule transmits signals that are important for T-cell activation and function. CD8 interacts with key proteins such as the T-cell receptor (TCR) and MHC class I molecules facilitating targeted responses against pathogens. These pathways highlight CD8 alpha’s role in adaptive immunity.

Associated diseases and disorders

CD8 alpha is most prominently associated with viral infections and cancer. Conditions like HIV and some forms of leukemia show altered CD8 function highlighting the protein's role in immune surveillance. In HIV infection for instance CD8 T cells reduce in number impairing the immune response. CD8 alpha’s connection to the immune system places it alongside other immune proteins such as CD4 and MHC molecules in the context of immune dysfunction.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [SP239] (ab178089), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [SP239] (ab178089)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CD8 alpha with ab178089 at followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on human tonsil, performed on a Leica Biosystems BOND® RX instrument. Counterstained with Paraffin-embedded sections. Hematoxylin Secondary antibody only control: Used PBS instead of primary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [SP239] (ab178089), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [SP239] (ab178089)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded human tonsil tissue labeling CD8 alpha with ab178089 at 1/100 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [SP239] (ab178089), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [SP239] (ab178089)

    Immunohistochemical analysis of formalin fixed paraffin embedded Human tonsil labelling CD8 with ab178089 at a concentration of 1 µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with a OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was performed with DISCOVERY cell conditioning solution (CC1) 100°C, pH8.5 for 32mins.

    ab178089 anti DCD8 antibody SP239 was incubated for 16mins at 37°C. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

    Customers are encouraged to optimise antigen retrieval conditions, antibody concentration, incubation times and temperature for best results in their own IHC assay workflow (automated and manual)

  • Multiplex immunohistochemistry - Anti-CD8 alpha antibody [SP239] (ab178089), expandable thumbnail

    Multiplex immunohistochemistry - Anti-CD8 alpha antibody [SP239] (ab178089)

    Multichannel (Multiplex) immunohistochemistry analysis of Formalin/PFA-fixed paraffin-embedded sections of human tonsil tissue labeling NKG2D, NKG2a, and CD8 alpha.

    Panel A: merged staining of anti-CD8 alpha (red; Opal™690), anti-NKG2A (gray; Opal™520) and anti-NKG2D (green; Opal™570) on human tonsil.

    Panel B: anti-NKG2D (Anti-NKG2D antibody [EPR24072-342] ab302907 at 1:2000 dilution (0.258 μg/ml)) signal on NK cells and T cell subsets.

    Panel C: anti-NKG2A (Anti-NKG2A antibody [EPR23737-127] ab260035 at 1:2000 dilution (0.283 μg/ml)) signal on NK cells.

    Panel D: anti-CD8 alpha (ab178089 at 1:100 dilution (0.83 μg/ml)) signal on T cell subsets.

    Opal Polymer HRP Ms + Rb was used as secondary antibody.

    The section was incubated in three rounds of staining: in the order of ab178089, Anti-NKG2A antibody [EPR23737-127] ab260035, and Anti-NKG2D antibody [EPR24072-342] ab302907 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. Nuclear counterstain was DAPI.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

  • Multiplex immunohistochemistry - Anti-CD8 alpha antibody [SP239] (ab178089), expandable thumbnail

    Multiplex immunohistochemistry - Anti-CD8 alpha antibody [SP239] (ab178089)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil labelling PD1 with Anti-PD1 antibody [EPR23119-111] ab243644 at 1/500 dilution (1.02 µg/mL) (D), CD8 with ab178089 at 1/100 dilution (0.83 μg/ml) (C) and TIM 3 with Anti-TIM 3 antibody [EPR22241] - BSA and Azide free ab242080 at 1/500 dilution (2.09 μg/ml) (B). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.  

    Panel A: merged staining of anti-TIM 3 (green; Opal™690), anti-CD8 (red; Opal™520) and anti-PD1 (gray; Opal™570) on human tonsil.
    Panel B: anti-TIM 3 stained on membrane of a subset of immune cells.
    Panel C: anti-CD8 stained on membrane of a subset of T cells.
    Panel D: anti-PD1 stained on membrane of a subset of lymphocytes.

    The section was incubated in three rounds of staining: in the order of Anti-TIM 3 antibody [EPR22241] - BSA and Azide free ab242080, Anti-PD1 antibody [EPR23119-111] ab243644 and ab178089 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com