AB60076

Anti-CD8 alpha antibody [YTC182.20]

Name: Anti-CD8 alpha antibody [YTC182.20] (ab60076) | Abcam
Brand: Abcam Limited
SKU: AB60076
Rating: 4 (1 reviews)

(1 Review)

(12 Publications)

Rat Monoclonal CD8 alpha antibody. Suitable for Flow Cyt and reacts with Human samples. Cited in 12 publications. Immunogen corresponding to Cell preparation containing CD8A protein.

View Alternative Names

CD8a, MAL, CD8A, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2

1 Images

Flow Cytometry - Anti-CD8 alpha antibody [YTC182.20] (AB60076)

Flow Cyt

Unknown

Flow Cytometry - Anti-CD8 alpha antibody [YTC182.20] (AB60076)

ab60076 at 1/100 dilution staining CD8 alpha in human peripheral blood lymphocytes by Flow Cytometry.

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

YTC182.20

Isotype

IgG2b

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing CD8A protein. The exact immunogen used to generate this antibody is proprietary information.

P01732

Specificity

Recognizes the human CD8 alpha cell surface antigen

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/100 - 1/200", "FlowCyt-species-notes": "Use 10μl of the suggested working dilution to label 106 cells in 100μl. ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody." }, "Cynomolgus monkey": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Rhesus monkey": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified from tissue culture supernatant.

Storage buffer

pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD8 alpha also known as CD8A or CD8 protein is a glycoprotein subunit expressed on the surface of cytotoxic T lymphocytes. It has a mass of approximately 32 kDa. Found on the surface cell membrane CD8 alpha functions primarily in the immune response specifically in the recognition of antigens bound to major histocompatibility complex (MHC) class I molecules. Often scientists use CD8 antibodies for detection and CD8 IHC or immunohistochemistry for localization studies.

Biological function summary

The CD8 alpha protein plays a critical role in T-cell mediated immune responses. It forms a heterodimer with the CD8 beta chain creating the CD8 alpha-beta complex that strengthens T-cell interaction with antigen-presenting cells. CD8 alpha also helps in signaling processes that activate T cells equipping them to destroy infected or malignant cells. Researchers often study CD8 alpha peptides to understand its interactions better.

Pathways

CD8 alpha is integral to the T-cell receptor signaling pathway and the cytotoxic T lymphocyte (CTL) pathway. The T-cell receptor complex which includes the CD8 molecule transmits signals that are important for T-cell activation and function. CD8 interacts with key proteins such as the T-cell receptor (TCR) and MHC class I molecules facilitating targeted responses against pathogens. These pathways highlight CD8 alpha’s role in adaptive immunity.

CD8 alpha is most prominently associated with viral infections and cancer. Conditions like HIV and some forms of leukemia show altered CD8 function highlighting the protein's role in immune surveillance. In HIV infection for instance CD8 T cells reduce in number impairing the immune response. CD8 alpha’s connection to the immune system places it alongside other immune proteins such as CD4 and MHC molecules in the context of immune dysfunction.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The protein expressed by the gene CD8A is an integral membrane glycoprotein crucial for immune responses, operating mainly in T-cells as a coreceptor for MHC class I molecule : peptide complexes. These peptides originate from cytosolic proteins, unlike class II peptides, which are from extracellular proteins. CD8A interacts with the T-cell receptor (TCR) and MHC class I proteins on antigen-presenting cells, recruiting the Src kinase LCK to the TCR-CD3 complex. LCK phosphorylates substrates, triggering signaling pathways that lead to the production of lymphokines, enhanced motility, adhesion, and activation of cytotoxic T-lymphocytes (CTLs), thereby aiding in the recognition and elimination of infected or tumor cells. Additionally, in natural killer (NK) cells, CD8A homodimers on the cell surface provide a survival mechanism for conjugating with and lysing multiple target cells. CD8A homodimers also facilitate the survival and differentiation of activated lymphocytes into memory CD8 T-cells. This supplementary information is collated from multiple sources and compiled automatically.

See full target information CD8A

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:5818 PubMed40595568

2025

PI3Kγ signaling controls trafficking of CD8 T cells between lymphoid and non-lymphoid organs and drives hypertension in a murine model.

Applications

Unspecified application

Species

Unspecified reactive species

Marialuisa Perrotta,Sara Perrotta,Lorenzo Carnevale,Agnese Migliaccio,Fabio Pallante,Ryszard Nosalski,Tomasz J Guzik,Stefania Fardella,Emilio Hirsch,Valentina Fardella,Azzurra Zonfrilli,Jacopo Pacella,Matthias P Wymann,Giuseppe Lembo,Daniela Carnevale

Scientific reports 15:10401 PubMed40140459

2025

Irradiated umbilical cord mesenchymal stem cell-coated high oxygen-permeable hydrogel lenses inhibit corneal inflammation and neovascularization after corneal alkali burns.

Applications

Unspecified application

Species

Unspecified reactive species

Siqi Song,Yaqi Cheng,Weihua Li,Huan Yu,Zhiquan Li,Jianbing Li,Meng Li,Qunai Huang,Yingjie Liu,Shiqi Ling

Nature 637:1207-1217 PubMed39633050

2024

Evolution of myeloid-mediated immunotherapy resistance in prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Aram Lyu,Zenghua Fan,Matthew Clark,Averey Lea,Diamond Luong,Ali Setayesh,Alec Starzinski,Rachel Wolters,Marcel Arias-Badia,Kate Allaire,Kai Wu,Vibha Gurunathan,Laura Valderrábano,Xiao X Wei,Richard A Miller,Eliezer M Van Allen,Lawrence Fong

Frontiers in immunology 12:647385 PubMed33777045

2021

Presence of Senescent and Memory CD8+ Leukocytes as Immunocenescence Markers in Skin Lesions of Elderly Leprosy Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Pedro Henrique Lopes da Silva,Katherine Kelda Gomes de Castro,Mayara Abud Mendes,Thyago Leal-Calvo,Júlia Monteiro Pereira Leal,José Augusto da Costa Nery,Euzenir Nunes Sarno,Roberto Alves Lourenço,Milton Ozório Moraes,Flávio Alves Lara,Danuza Esquenazi

PLoS neglected tropical diseases 15:e0009214 PubMed33690671

2021

Increased oxidative stress in elderly leprosy patients is related to age but not to bacillary load.

Applications

Unspecified application

Species

Unspecified reactive species

Pedro Henrique Lopes da Silva,Katherine Kelda Gomes de Castro,Mayara Abud Mendes,Thyago Leal Calvo,Júlia Monteiro Pereira Leal,Mariana de Andréa Vilas-Boas Hacker,José Augusto da Costa Nery,Euzenir Nunes Sarno,Roberto Alves Lourenço,Milton Ozório Moraes,Flávio Alves Lara,Danuza Esquenazi

Frontiers in immunology 11:579776 PubMed33329550

2020

CD4CD8 T-Lymphocytes in Xenogeneic and Human Graft-versus-Host Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Kutaiba Alhaj Hussen,David Michonneau,Vincent Biajoux,Seydou Keita,Laetitia Dubouchet,Elisabeth Nelson,Niclas Setterblad,Helene Le Buanec,Jean-David Bouaziz,Fabien Guimiot,Gérard Socié,Bruno Canque

Clinical cancer research : an official journal of 26:2231-2243 PubMed31953311

2020

Surfactant Expression Defines an Inflamed Subtype of Lung Adenocarcinoma Brain Metastases that Correlates with Prolonged Survival.

Applications

Unspecified application

Species

Unspecified reactive species

Kolja Pocha,Andreas Mock,Carmen Rapp,Steffen Dettling,Rolf Warta,Christoph Geisenberger,Christine Jungk,Leila R Martins,Niels Grabe,David Reuss,Juergen Debus,Andreas von Deimling,Amir Abdollahi,Andreas Unterberg,Christel C Herold-Mende

Clinical cancer research : an official journal of 25:5260-5270 PubMed31227506

2019

Cytotoxic T Cells and their Activation Status are Independent Prognostic Markers in Meningiomas.

Applications

Unspecified application

Species

Unspecified reactive species

Carmen Rapp,Steffen Dettling,Fang Liu,Anna Theresa Ull,Rolf Warta,Christine Jungk,Saskia Roesch,Andreas Mock,Felix Sahm,Melissa Schmidt,Gerhard Jungwirth,Klaus Zweckberger,Katrin Lamszus,Konstaninos Gousias,Almuth F Kessler,Niels Grabe,Mario Loehr,Ralf Ketter,Steffi Urbschat,Christian Senft,Manfred Westphal,Amir Abdollahi,Juergen Debus,Andreas von Deimling,Andreas Unterberg,Matthias Simon,Christel C Herold-Mende

Cell metabolism 28:946-960.e6 PubMed30078552

2018

Conventional and Neo-antigenic Peptides Presented by β Cells Are Targeted by Circulating Naïve CD8+ T Cells in Type 1 Diabetic and Healthy Donors.

Applications

Unspecified application

Species

Unspecified reactive species

Sergio Gonzalez-Duque,Marie Eliane Azoury,Maikel L Colli,Georgia Afonso,Jean-Valery Turatsinze,Laura Nigi,Ana Ines Lalanne,Guido Sebastiani,Alexia Carré,Sheena Pinto,Slobodan Culina,Noémie Corcos,Marco Bugliani,Piero Marchetti,Mathieu Armanet,Marc Diedisheim,Bruno Kyewski,Lars M Steinmetz,Søren Buus,Sylvaine You,Daniele Dubois-Laforgue,Etienne Larger,Jean-Paul Beressi,Graziella Bruno,Francesco Dotta,Raphael Scharfmann,Decio L Eizirik,Yann Verdier,Joelle Vinh,Roberto Mallone

Nature communications 9:1241 PubMed29593314

2018

Oncofetal gene SALL4 reactivation by hepatitis B virus counteracts miR-200c in PD-L1-induced T cell exhaustion.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng Sun,Peixiang Lan,Qiuju Han,Mei Huang,Zhihong Zhang,Geliang Xu,Jiaxi Song,Jinyu Wang,Haiming Wei,Jian Zhang,Rui Sun,Cai Zhang,Zhigang Tian

View all publications

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