Anti-CD8 alpha antibody [YTS169.4]

• Flow Cyt

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Flow Cytometry - Anti-CD8 alpha antibody [YTS169.4] (AB22378)

Overlay histogram showing mouse spleen cells (C57BL/6) labeling CD8 alpha with ab22378 (red line). The cells were incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab22378, 0.1μg/1x10⁶ cells) for 30 min at 4°C. The secondary antibody used was Alexa Fluor^® 488 goat anti-rat IgG (H&L) (ab150157) at 1/2000 dilution for 30 min at 4°C. Isotype control antibody (black line) was rat IgG2b [RTK4530] (ab18541, 2μg/1x10⁶ cells) used under the same conditions.

Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

• Flow Cyt

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Flow Cytometry - Anti-CD8 alpha antibody [YTS169.4] (AB22378)

Normal mouse (CBA) spleen cells (red cell lysed) double stained with ab22378 (0.3 μg/mL, detected with Alexa Fluor^® 488-conjugated monoclonal mouse anti-rat antibody) and an APC conjugated hamster anti-mouse CD3 antibody. ab22378 stains an expected subpopulation of CD8 alpha positive T cells.

• WB

CiteAb

Western blot - Anti-CD8 alpha antibody [YTS169.4] (AB22378)

Western Blotting using Anti-CD8 alpha antibody [YTS169.4], ab22378. Publication image from Lin, W. et al., 2020, Theranostics, 32308755. Legend direct from paper.

Characterization of small extracellular vesicles (sEVs). (A) Particle size distribution measured via nanoparticle tracking analysis (NTA). (B) sEV morphology revealed via transmission electron microscopy (TEM). (C) Western blot analysis of specific sEV-specific surface markers.

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