AB185762

Anti-CD8 beta antibody [341] - Low endotoxin, Azide free

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(1 Publication)

Mouse Monoclonal CD8 beta antibody. Carrier free. Suitable for Flow Cyt and reacts with Rat samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing Cd8b protein.

View Alternative Names

CD8b, Cd8b1, Cd8b, T-cell surface glycoprotein CD8 beta chain, CD8 antigen 37 kDa chain, OX-8 membrane antigen

1 Images

Flow Cytometry - Anti-CD8 beta antibody [341] - Low endotoxin, Azide free (AB185762)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD8 beta antibody [341] - Low endotoxin, Azide free (AB185762)

Staining of Lou rat splenocytes with 0.25 ug of Mouse IgG1 kappa isotype control (blue histogram) or 0.25 ug of ab185762 (purple histogram) followed by an F(ab')2 anti-mouse IgG PE. Total viable cells were used for analysis.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

341

Isotype

IgG1

Light chain type

kappa

Carrier free

Yes

Reacts with

Rat

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing Cd8b protein. The exact immunogen used to generate this antibody is proprietary information.

P05541

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "This antibody can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 105 to 108 cells/test.<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.

Storage buffer

pH: 7.2 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The target CD8 beta also known as CD8b or CD8B is a protein component of the CD8 coreceptor that is primarily expressed on the surface of cytotoxic T lymphocytes and a subset of natural killer cells. The CD8 beta molecule pairs with CD8 alpha to form a heterodimer that plays an essential role in the immune response. The molecular weight of CD8 beta is approximately 34 kDa. It is located primarily in the plasma membrane where it interacts with major histocompatibility complex (MHC) class I molecules.

Biological function summary

The CD8 beta protein works as part of the CD8 coreceptor complex which enhances the interaction between T cell receptors (TCRs) and antigens presented by MHC class I on infected or malignant cells. This interaction strengthens the activation signal received by T cells. The CD8 complex including CD8 beta contributes to the recognition and elimination of cells presenting foreign antigens which is essential for the adaptive immune response.

Pathways

The CD8 beta protein participates in pathways related to antigen recognition and T cell receptor signaling. The protein together with CD8 alpha facilitates signal transduction following TCR engagement. This action is vital for the activation of cytotoxic T cells enabling immune responses. CD8 beta associates with the Lck kinase another vital player in the TCR signaling pathway through its cytoplasmic tail. In this pathway the presence of CD8 beta helps maintain and modulate effective immune responses.

CD8 beta plays a significant role in conditions like viral infections and autoimmune diseases. In viral infections the presence of the CD8 complex containing CD8 beta aids in the destruction of virus-infected cells. In autoimmune diseases abnormal CD8 T cell responses can result in self-tissue damage reflecting dysregulation in its normal function. CD8 beta therefore proves critical in these contexts contributing to disease dynamics by interacting with proteins like MHC class I and Lck kinase during improper immune responses.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Visit the Troubleshooting

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Target data

Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule : peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. A palmitoylation site in the cytoplasmic tail of CD8B chain contributes to partitioning of CD8 into the plasma membrane lipid rafts where signaling proteins are enriched. Once LCK recruited, it initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs). Additionally, plays a critical role in thymic selection of CD8+ T-cells.

See full target information Cd8b

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in genetics 13:771344 PubMed35646093

2022

An Integrated Systematic Analysis and the Clinical Significance of Hepcidin in Common Malignancies of the Male Genitourinary System.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaogang Wang,Qianqian Shi,Pengfeng Gong,Cuixing Zhou,Yunjie Cao

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