AB228965

Anti-CD8 beta antibody [EPR22331-54]

Name: Anti-CD8 beta antibody [EPR22331-54] Rabbit monoclonal (ab228965) | Abcam
Brand: Abcam Limited
SKU: AB228965
Price: 250 USD
Availability: InStock
Rating: 5 (1 reviews)

BOND RX™ Validated
RabMAb
Recombinant
What is this?

(1 Review)

(8 Publications)

Rabbit Recombinant Monoclonal CD8 beta antibody. Suitable for IP, WB, IHC-Fr, IHC-P and reacts with Mouse samples. Cited in 8 publications.

View Alternative Names

CD8b, Cd8b1, Ly-3, Lyt-3, Lyt3, Cd8b, T-cell surface glycoprotein CD8 beta chain, Lymphocyte antigen 3, T-cell membrane glycoprotein Ly-3, T-cell surface glycoprotein Lyt-3

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 beta antibody [EPR22331-54] (AB228965)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 beta antibody [EPR22331-54] (AB228965)

Immunohistochemical analysis of paraffin-embedded mouse thymus tissue labeling CD8 beta with ab228965 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on T cells in the cortex of mouse thymus (PMID : 12702501) is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 minutes.

The section was incubated with ab228965 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 beta antibody [EPR22331-54] (AB228965)

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD8 beta with ab228965 at 1/500 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on T cells in periarterial lymphatic sheath of mouse spleen (PMID : 18776164) is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, the ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 minutes.

The section was incubated with ab228965 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

Immunohistochemistry (Frozen sections) - Anti-CD8 beta antibody [EPR22331-54] (AB228965)

IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-CD8 beta antibody [EPR22331-54] (AB228965)

Frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized mouse thymus tissue stained for CD8 beta (green) using ab228965 at 1/100 dilution in immunohistochemical analysis. The secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution. Specific membrane staining on T cells in the cortex of mouse thymus (PMID 12702501) is observed. The nuclear conter stain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Unknown

Immunoprecipitation - Anti-CD8 beta antibody [EPR22331-54] (AB228965)

CD8 beta was immunoprecipitated from 0.35 mg of mouse thymus lysate with ab228965 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab228965 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/5000 dilution.

Lane 1 : Mouse thymus lysate 10 μg (Input).
Lane 2 : ab228965 IP in mouse thymus lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab22896 in mouse thymus lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 3 seconds.

All lanes:

Immunoprecipitation - Anti-CD8 beta antibody [EPR22331-54] (ab228965)

Predicted band size: 21 kDa,23 kDa,24 kDa,28 kDa

Observed band size: 21 kDa,28 kDa,28-32 kDa

false

Unknown

Western blot - Anti-CD8 beta antibody [EPR22331-54] (AB228965)

Blocking and dilution buffer : 5% NFDM/TBST.

CD8 beta is a glycoprotein. The molecular weight observed is consistent with what has been described in the literature (PMID : 11719190).

Exposure times :
Lanes 1-2 : 37 seconds.
Lane 3 : 6 seconds.

All lanes:

Western blot - Anti-CD8 beta antibody [EPR22331-54] (ab228965) at 1/1000 dilution

Lane 1:

Mouse spleen lysate at 10 µg

Lane 2:

Mouse lymph node lysate at 10 µg

Lane 3:

Mouse thymus lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 24 kDa

Observed band size: 28-32 kDa

false

Carrier free

Anti-CD8 beta antibody [EPR22331-54] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22331-54

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

IHC-Fr, IHC-P, WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100", "IHCFr-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The target CD8 beta also known as CD8b or CD8B is a protein component of the CD8 coreceptor that is primarily expressed on the surface of cytotoxic T lymphocytes and a subset of natural killer cells. The CD8 beta molecule pairs with CD8 alpha to form a heterodimer that plays an essential role in the immune response. The molecular weight of CD8 beta is approximately 34 kDa. It is located primarily in the plasma membrane where it interacts with major histocompatibility complex (MHC) class I molecules.

Biological function summary

The CD8 beta protein works as part of the CD8 coreceptor complex which enhances the interaction between T cell receptors (TCRs) and antigens presented by MHC class I on infected or malignant cells. This interaction strengthens the activation signal received by T cells. The CD8 complex including CD8 beta contributes to the recognition and elimination of cells presenting foreign antigens which is essential for the adaptive immune response.

Pathways

The CD8 beta protein participates in pathways related to antigen recognition and T cell receptor signaling. The protein together with CD8 alpha facilitates signal transduction following TCR engagement. This action is vital for the activation of cytotoxic T cells enabling immune responses. CD8 beta associates with the Lck kinase another vital player in the TCR signaling pathway through its cytoplasmic tail. In this pathway the presence of CD8 beta helps maintain and modulate effective immune responses.

CD8 beta plays a significant role in conditions like viral infections and autoimmune diseases. In viral infections the presence of the CD8 complex containing CD8 beta aids in the destruction of virus-infected cells. In autoimmune diseases abnormal CD8 T cell responses can result in self-tissue damage reflecting dysregulation in its normal function. CD8 beta therefore proves critical in these contexts contributing to disease dynamics by interacting with proteins like MHC class I and Lck kinase during improper immune responses.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule : peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. A palmitoylation site in the cytoplasmic tail of CD8B chain contributes to partitioning of CD8 into the plasma membrane lipid rafts where signaling proteins are enriched. Once LCK recruited, it initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs). Additionally, plays a critical role in thymic selection of CD8+ T-cells.

See full target information Cd8b

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Cancer discovery 15:1905-1926 PubMed40498998

2025

Gut Microbiota Modulation through Akkermansia spp. Supplementation Increases CAR T-cell Potency.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Marcos-Kovandzic,Michele Avagliano,Myriam Ben Khelil,Janesa Srikanthan,Rim Abdallah,Valentina Petrocelli,Jessica Rengassamy,Alexia Alfaro,Mathilde Bied,Marine Fidelle,Gladys Ferrere,Romain Daillère,Ahmadreza Arbab,Roula Amine-Hneineh,Arnaud Pages,Peggy Dartigues,Pierre Ly,Sylvain Simon,Sylvère Durand,Adrian Gottschlich,Florent Ginhoux,Camille Blériot,Peng Liu,Liwei Zhao,Laura Creusot,Nathalie Rolhion,Lisa Derosa,Guido Kroemer,Laurie Menger,Sebastian Kobold,Cristina Castilla-Llorente,Harry Sokol,Stefano Casola,Edoardo Pasolli,Laurence Zitvogel,Camille Bigenwald

Research (Washington, D.C.) 8:0673 PubMed40342298

2025

Isobutyrate Confers Resistance to Inflammatory Bowel Disease through Host-Microbiota Interactions in Pigs.

Applications

Unspecified application

Species

Unspecified reactive species

Xiuyu Fang,Haiyang Liu,Junling Liu,Yongqing Du,Zihan Chi,Yiqi Bian,Xuan Zhao,Teng Teng,Baoming Shi

Frontiers in immunology 16:1512186 PubMed40270974

2025

Exploring the role of LOX family in glioma progression and immune modulation.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Liu,Huilian Qiao,Hongqi Li,Xiaolong Hu,Maohui Yan,Zhiguang Fu,Hengheng Zhang,Yingjie Wang,Nan Du

Frontiers in oncology 14:1454291 PubMed39723364

2024

Prolonged survival by combination treatment with a standardized herbal extract from Japanese Kampo-medicine (Juzentaihoto) and gemcitabine in an orthotopic transplantation pancreatic cancer model.

Applications

Unspecified application

Species

Unspecified reactive species

Joanna Napp,Paulina Siebel,Hans Rausch,Kenny Kuchta,Thomas Efferth,Frauke Alves,Volker Ellenrieder,Silke Cameron

Molecular medicine reports 31: PubMed39540364

2024

Integrin β2 regulates titanium particle‑induced inflammation in macrophages: aseptic loosening model.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Shen,Haruna Nakajima,Junfeng Zhu,Weigang Wu

Naunyn-Schmiedeberg's archives of pharmacology 397:8023-8041 PubMed38775851

2024

Interleukin 1β receptor blocker (Anakinra) and regenerative stem cell therapy: two novel approaches effectively ameliorating diabetic cardiomyopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Lamiaa Mohamed Mahmoud,Aya Aly Ashraf Abdel Mageed,Jackline Moawad Saadallah,Mira Farouk Youssef,Liala Ahmed Rashed,Hania Ibrahim Ammar

Frontiers in immunology 13:863346 PubMed35874730

2022

Orthotopic and Heterotopic Murine Models of Pancreatic Cancer Exhibit Different Immunological Microenvironments and Different Responses to Immunotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Jin Wang,Xingchen Liu,Junsong Ji,Jianhua Luo,Yuanyu Zhao,Xiaonan Zhou,Jianming Zheng,Meng Guo,Yanfang Liu

Frontiers in immunology 13:860311 PubMed35514982

2022

Single-Cell Characterization of Hepatic CD8 T Cells in a Murine Model of Primary Biliary Cholangitis.

Applications

Unspecified application

Species

Unspecified reactive species

Yichen Han,Zhen-Hua Bian,Si-Yu Yang,Cheng-Bo Wang,Liang Li,Yan-Qing Yang,Aftab A Ansari,M Eric Gershwin,Xiaofeng Zeng,Zhe-Xiong Lian,Zhi-Bin Zhao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD8 beta antibody [EPR22331-54]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 beta antibody [EPR22331-54] (AB228965)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 beta antibody [EPR22331-54] (AB228965)

Immunohistochemistry (Frozen sections) - Anti-CD8 beta antibody [EPR22331-54] (AB228965)

Immunoprecipitation - Anti-CD8 beta antibody [EPR22331-54] (AB228965)

Western blot - Anti-CD8 beta antibody [EPR22331-54] (AB228965)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

Product promise