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AB263946

Anti-CD8 beta antibody [EPR22331-81]

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(3 Publications)

Rabbit Recombinant Monoclonal CD8 beta antibody. Suitable for IP, Flow Cyt, WB, IHC-Fr and reacts with Mouse samples. Cited in 3 publications.

View Alternative Names

CD8b, Cd8b1, Ly-3, Lyt-3, Lyt3, Cd8b, T-cell surface glycoprotein CD8 beta chain, Lymphocyte antigen 3, T-cell membrane glycoprotein Ly-3, T-cell surface glycoprotein Lyt-3

5 Images
Flow Cytometry - Anti-CD8 beta antibody [EPR22331-81] (AB263946)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-CD8 beta antibody [EPR22331-81] (AB263946)

Flow Cytometric analysis of mouse thymocytes using ab263946 at a 1/500 dilution. Cells were stained with Rabbit monoclonal IgG (ab172730) (Left) or ab263946 (Right). Then stained with anti-CD4 conjugated to Alexa Fluor® 647. Secondary used is Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at a 1/2000 dilution.

Gated on viable cells.

Immunohistochemistry (Frozen sections) - Anti-CD8 beta antibody [EPR22331-81] (AB263946)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-CD8 beta antibody [EPR22331-81] (AB263946)

Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen mouse thymus tissue labeling CD8 beta with ab263946 at 1/100 (5.13 µg/ml) dilution followed by ab150077 Alexa Fluor® 488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution. The nuclear counterstain was DAPI (Blue). Positive staining on T cells of mouse thymus (PMID : 12702501) is observed.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody was ab150077 Alexa Fluor® 488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution.

Immunohistochemistry (Frozen sections) - Anti-CD8 beta antibody [EPR22331-81] (AB263946)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-CD8 beta antibody [EPR22331-81] (AB263946)

Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen mouse liver tissue labeling CD8 beta with ab263946 at 1/100 (5.13 µg/ml) dilution followed by ab150077 Alexa Fluor® 488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution. The nuclear counterstain was DAPI (Blue). Negative staining on mouse liver.

Negative control : liver (PMID : 3145195).

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody was ab150077 Alexa Fluor® 488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution.

Immunoprecipitation - Anti-CD8 beta antibody [EPR22331-81] (AB263946)
  • IP

Supplier Data

Immunoprecipitation - Anti-CD8 beta antibody [EPR22331-81] (AB263946)

CD8 beta was immunoprecipitated from 0.35 mg mouse thymus lysate 10μg with ab263946 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab263946 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : Mouse thymus lysate 10μg
Lane 2 : ab263946 IP in mouse thymus lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab263946 in mouse thymus lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 10 seconds.

All lanes:

Immunoprecipitation - Anti-CD8 beta antibody [EPR22331-81] (ab263946)

Predicted band size: 24 kDa

false

Western blot - Anti-CD8 beta antibody [EPR22331-81] (AB263946)
  • WB

Lab

Western blot - Anti-CD8 beta antibody [EPR22331-81] (AB263946)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

CD8 beta is a glycoprotein. The molecular weight observed is consistent with what has been described in the literature (PMID : 11719190).

Exposure time :
Lane 1 : 3 secs.
Lane 2 : 3 mins.

All lanes:

Western blot - Anti-CD8 beta antibody [EPR22331-81] (ab263946) at 1/1000 dilution

Lane 1:

Mouse thymus lysate at 20 µg

Lane 2:

Mouse lymph node lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 24 kDa

Observed band size: 28-32 kDa

false

  • Carrier free

    Anti-CD8 beta antibody [EPR22331-81] - BSA and Azide free

  • 660 APC

    APC Anti-CD8 antibody [EPR22331-81]

  • 578 PE

    PE Anti-CD8 antibody [EPR22331-81]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22331-81

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

IHC-Fr, WB, Flow Cyt, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/500", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100", "IHCFr-species-notes": "<p>Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).</p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The target CD8 beta also known as CD8b or CD8B is a protein component of the CD8 coreceptor that is primarily expressed on the surface of cytotoxic T lymphocytes and a subset of natural killer cells. The CD8 beta molecule pairs with CD8 alpha to form a heterodimer that plays an essential role in the immune response. The molecular weight of CD8 beta is approximately 34 kDa. It is located primarily in the plasma membrane where it interacts with major histocompatibility complex (MHC) class I molecules.
Biological function summary

The CD8 beta protein works as part of the CD8 coreceptor complex which enhances the interaction between T cell receptors (TCRs) and antigens presented by MHC class I on infected or malignant cells. This interaction strengthens the activation signal received by T cells. The CD8 complex including CD8 beta contributes to the recognition and elimination of cells presenting foreign antigens which is essential for the adaptive immune response.

Pathways

The CD8 beta protein participates in pathways related to antigen recognition and T cell receptor signaling. The protein together with CD8 alpha facilitates signal transduction following TCR engagement. This action is vital for the activation of cytotoxic T cells enabling immune responses. CD8 beta associates with the Lck kinase another vital player in the TCR signaling pathway through its cytoplasmic tail. In this pathway the presence of CD8 beta helps maintain and modulate effective immune responses.

CD8 beta plays a significant role in conditions like viral infections and autoimmune diseases. In viral infections the presence of the CD8 complex containing CD8 beta aids in the destruction of virus-infected cells. In autoimmune diseases abnormal CD8 T cell responses can result in self-tissue damage reflecting dysregulation in its normal function. CD8 beta therefore proves critical in these contexts contributing to disease dynamics by interacting with proteins like MHC class I and Lck kinase during improper immune responses.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule : peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. A palmitoylation site in the cytoplasmic tail of CD8B chain contributes to partitioning of CD8 into the plasma membrane lipid rafts where signaling proteins are enriched. Once LCK recruited, it initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs). Additionally, plays a critical role in thymic selection of CD8+ T-cells.
See full target information Cd8b
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

F1000Research 12:745 PubMed38638178

2024

A guide to selecting high-performing antibodies for RNA-binding protein TIA1 for use in Western Blot, immunoprecipitation and immunofluorescence.

Applications

Unspecified application

Species

Unspecified reactive species

Maryam Fotouhi,Donovan Worrall,Riham Ayoubi,Kathleen Southern,Peter S McPherson,Carl Laflamme

Theranostics 12:5488-5503 PubMed35910792

2022

Co-delivery of phagocytosis checkpoint and STING agonist by a Trojan horse nanocapsule for orthotopic glioma immunotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Zhou,Yuxin Guo,Lufei Chen,Xiaoli Zhang,Wei Wu,Zhimin Yang,Xuejie Li,Yuanzhuo Wang,Zhiyuan Hu,Zihua Wang

Aging 13:13006-13022 PubMed33910164

2021

A preclinical study: correlation between PD-L1 PET imaging and the prediction of therapy efficacy of MC38 tumor with Ga-labeled PD-L1 targeted nanobody.

Applications

Unspecified application

Species

Unspecified reactive species

Songbing Qin,Yang Yu,Hui Guan,Yanling Yang,Fenghao Sun,Yan Sun,Jiaxing Zhu,Ligang Xing,Jinming Yu,Xiaorong Sun
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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