Skip to main content

Rabbit Recombinant Monoclonal CD8 beta antibody. Suitable for IP, Flow Cyt, WB, IHC-Fr and reacts with Mouse samples. Cited in 1 publication.

Be the first to review this product! Submit a review

Images

Western blot - Anti-CD8 beta antibody [EPR22331-81] (AB263946), expandable thumbnail
  • Immunoprecipitation - Anti-CD8 beta antibody [EPR22331-81] (AB263946), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-CD8 beta antibody [EPR22331-81] (AB263946), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-CD8 beta antibody [EPR22331-81] (AB263946), expandable thumbnail
  • Flow Cytometry - Anti-CD8 beta antibody [EPR22331-81] (AB263946), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBIHC-PIHC-Fr
Mouse
Tested
Tested
Tested
Not recommended
Tested

Tested
Tested

Species
Mouse
Dilution info
1/30
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/500
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/100
Notes

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Associated Products

Select an associated product type

5 products for Alternative Product

Target data

Function

Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. A palmitoylation site in the cytoplasmic tail of CD8B chain contributes to partitioning of CD8 into the plasma membrane lipid rafts where signaling proteins are enriched. Once LCK recruited, it initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs). Additionally, plays a critical role in thymic selection of CD8+ T-cells.

Alternative names

CD8b, Cd8b1, Ly-3, Lyt-3, Lyt3, Cd8b, T-cell surface glycoprotein CD8 beta chain, Lymphocyte antigen 3, T-cell membrane glycoprotein Ly-3, T-cell surface glycoprotein Lyt-3

Recommended products

Rabbit Recombinant Monoclonal CD8 beta antibody. Suitable for IP, Flow Cyt, WB, IHC-Fr and reacts with Mouse samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR22331-81
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The target CD8 beta also known as CD8b or CD8B is a protein component of the CD8 coreceptor that is primarily expressed on the surface of cytotoxic T lymphocytes and a subset of natural killer cells. The CD8 beta molecule pairs with CD8 alpha to form a heterodimer that plays an essential role in the immune response. The molecular weight of CD8 beta is approximately 34 kDa. It is located primarily in the plasma membrane where it interacts with major histocompatibility complex (MHC) class I molecules.

Biological function summary

The CD8 beta protein works as part of the CD8 coreceptor complex which enhances the interaction between T cell receptors (TCRs) and antigens presented by MHC class I on infected or malignant cells. This interaction strengthens the activation signal received by T cells. The CD8 complex including CD8 beta contributes to the recognition and elimination of cells presenting foreign antigens which is essential for the adaptive immune response.

Pathways

The CD8 beta protein participates in pathways related to antigen recognition and T cell receptor signaling. The protein together with CD8 alpha facilitates signal transduction following TCR engagement. This action is vital for the activation of cytotoxic T cells enabling immune responses. CD8 beta associates with the Lck kinase another vital player in the TCR signaling pathway through its cytoplasmic tail. In this pathway the presence of CD8 beta helps maintain and modulate effective immune responses.

Associated diseases and disorders

CD8 beta plays a significant role in conditions like viral infections and autoimmune diseases. In viral infections the presence of the CD8 complex containing CD8 beta aids in the destruction of virus-infected cells. In autoimmune diseases abnormal CD8 T cell responses can result in self-tissue damage reflecting dysregulation in its normal function. CD8 beta therefore proves critical in these contexts contributing to disease dynamics by interacting with proteins like MHC class I and Lck kinase during improper immune responses.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-CD8 beta antibody [EPR22331-81] (ab263946), expandable thumbnail

    Western blot - Anti-CD8 beta antibody [EPR22331-81] (ab263946)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    CD8 beta is a glycoprotein. The molecular weight observed is consistent with what has been described in the literature (PMID: 11719190).

    Exposure time:
    Lane 1: 3 secs.
    Lane 2: 3 mins.

    All lanes: Western blot - Anti-CD8 beta antibody [EPR22331-81] (ab263946) at 1/1000 dilution

    Lane 1: Mouse thymus lysate at 20 µg

    Lane 2: Mouse lymph node lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 24 kDa

    Observed band size: 28-32 kDa

  • Immunoprecipitation - Anti-CD8 beta antibody [EPR22331-81] (ab263946), expandable thumbnail

    Immunoprecipitation - Anti-CD8 beta antibody [EPR22331-81] (ab263946)

    CD8 beta was immunoprecipitated from 0.35 mg mouse thymus lysate 10μg with ab263946 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab263946 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse thymus lysate 10μg
    Lane 2: ab263946 IP in mouse thymus lysate
    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab263946 in mouse thymus lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

    All lanes: Immunoprecipitation - Anti-CD8 beta antibody [EPR22331-81] (ab263946)

    Predicted band size: 24 kDa

  • Immunohistochemistry (Frozen sections) - Anti-CD8 beta antibody [EPR22331-81] (ab263946), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-CD8 beta antibody [EPR22331-81] (ab263946)

    Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen mouse liver tissue labeling CD8 beta with ab263946 at 1/100 (5.13 µg/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Alexa Fluor® 488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution. The nuclear counterstain was DAPI (Blue). Negative staining on mouse liver.

    Negative control: liver (PMID: 3145195).

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Alexa Fluor® 488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution.

  • Immunohistochemistry (Frozen sections) - Anti-CD8 beta antibody [EPR22331-81] (ab263946), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-CD8 beta antibody [EPR22331-81] (ab263946)

    Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen mouse thymus tissue labeling CD8 beta with ab263946 at 1/100 (5.13 µg/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Alexa Fluor® 488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution. The nuclear counterstain was DAPI (Blue). Positive staining on T cells of mouse thymus (PMID: 12702501) is observed.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Alexa Fluor® 488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution.

  • Flow Cytometry - Anti-CD8 beta antibody [EPR22331-81] (ab263946), expandable thumbnail

    Flow Cytometry - Anti-CD8 beta antibody [EPR22331-81] (ab263946)

    Flow Cytometric analysis of mouse thymocytes using ab263946 at a 1/500 dilution. Cells were stained with Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Left) or ab263946 (Right). Then stained with anti-CD4 conjugated to Alexa Fluor® 647. Secondary used is Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at a 1/2000 dilution.

    Gated on viable cells.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com