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AB106162

Anti-CD80 antibody [16-10A1]

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(8 Publications)

Armenian hamster Monoclonal CD80 antibody. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 8 publications. Immunogen corresponding to Cell preparation containing Cd80 protein.

View Alternative Names

CD80, B7, Cd80, T-lymphocyte activation antigen CD80, Activation B7-1 antigen

1 Images
Flow Cytometry - Anti-CD80 antibody [16-10A1] (AB106162)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD80 antibody [16-10A1] (AB106162)

Flow cytometric analysis of C57BI/6 splenocytes stimulated with anti-IgM and anti-CD40 for 4 days. Cells were stained with 0.06 μg ab106162 (blue line) or 0.06 μg APC Armenian Hamster isotype control (dashed line).

  • 660 APC

    APC Anti-CD80 antibody [16-10A1]

  • 578 PE

    PE Anti-CD80 antibody [16-10A1]

  • 519 FITC

    FITC Anti-CD80 antibody [16-10A1]

  • 667 PE/Cy5®

    PE/Cy5® Anti-CD80 antibody [16-10A1]

Key facts

Host species

Armenian hamster

Clonality

Monoclonal

Clone number

16-10A1

Isotype

IgG

Light chain type

kappa

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing Cd80 protein. The exact immunogen used to generate this antibody is proprietary information.

Q00609

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purity
undefined SDS-PAGE
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: PBS, 0.87% Sodium dihydrogen phosphate, 0.14% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD80 also known as B7-1 is a cell surface protein with a molecular weight of approximately 60 kDa. It is expressed on antigen-presenting cells such as dendritic cells macrophages and B cells. The CD80 protein plays a mechanical role in immune response modulation by providing necessary signals for T cell activation and survival. CD80 interacts with receptors CD28 and CTLA-4 on T cells serving as a costimulatory molecule important for productive immune responses.
Biological function summary

CD80 plays a significant role in the immune system's ability to distinguish between self and non-self. It belongs to the B7 family and participates in the formation of immunological synapses between T cells and antigen-presenting cells. This interaction is essential for triggering T cell proliferation and cytokine production forming a part of the adaptive immune response. CD80's expression increases in response to pathogen recognition enhancing the immune system's efficiency.

Pathways

CD80 is integral to the regulation of T cell-mediated immune responses. It is involved in the CD28 co-stimulatory pathway which is vital for T cell activation and function. In this pathway CD80 interacts with proteins like CD28 and CTLA-4 to modulate immune activation and regulate immune tolerance. The proper function of CD80 within this pathway ensures a balanced immune reaction preventing autoimmunity while allowing effective defense against pathogens.

CD80 plays a role in autoimmune conditions and transplantation rejection. Its overexpression or dysregulation can contribute to diseases such as rheumatoid arthritis where it modifies T cell response and inflammation. Additionally CD80 is implicated in organ transplantation influencing graft rejection outcomes due to its role in modulating immune activation. Understanding CD80 interactions with CTLA-4 and CD28 offers insight into these conditions providing potential targets for therapeutic intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Costimulatory molecule that belongs to the immunoglobulin superfamily that plays an important role in T-lymphocyte activation. Acts as the primary auxiliary signal augmenting the MHC/TCR signal in naive T-cells together with the CD28 receptor which is constitutively expressed on the cell surface of T-cells (PubMed : 16339518). In turn, activates different signaling pathways such as NF-kappa-B or MAPK leading to the production of different cytokines. In addition, CD28/CD80 costimulatory signal stimulates glucose metabolism and ATP synthesis of T-cells by activating the PI3K/Akt signaling pathway. Acts also as a regulator of PDL1/PDCD1 interactions to limit excess engagement of PDL1 and its inhibitory role in immune responses. Expressed on B-cells, plays a critical role in regulating interactions between B-cells and T-cells in both early and late germinal center responses, which are crucial for the generation of effective humoral immune responses (PubMed : 22450810).
See full target information Cd80
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in molecular medicine 4:1487526 PubMed39834851

2025

Immune-checkpoint-inhibitor therapy directed against PD-L1 is tolerated in the heart without manifestation of cardiac inflammation in a preclinical reversible melanoma mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Caroline Schoenherr,Stefan Pietzsch,Cristina Barca,Franziska E Müller,Frauke S Bahr,Martina Kasten,Andre Zeug,Sergej Erschow,Christine S Falk,Evgeni Ponimaskin,James T Thackeray,Denise Hilfiker-Kleiner,Melanie Ricke-Hoch

Experimental and therapeutic medicine 20:1541-1549 PubMed32742385

2020

Mechanism of TGF-β1 inhibiting Kupffer cell immune responses in cholestatic cirrhosis.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Qian,Yuwen Jiao,Guangyao Wang,Hanyang Liu,Xiang Cao,Haojun Yang

Journal of leukocyte biology 80:66-74 PubMed16624934

2006

Modulation of cell cycle progression by CTLA4-CD80/CD86 interactions on CD4+ T cells depends on strength of the CD3 signal: critical role for IL-2.

Applications

Unspecified application

Species

Unspecified reactive species

Sambuddho Mukherjee,Asma Ahmed,Shruti Malu,Dipankar Nandi

Journal of the American Society of Nephrology : JA 16:2012-22 PubMed15944341

2005

Glomerular expression of CD80 and CD86 is required for leukocyte accumulation and injury in crescentic glomerulonephritis.

Applications

Unspecified application

Species

Unspecified reactive species

Dragana Odobasic,A Richard Kitching,Timothy J Semple,Jennifer R Timoshanko,Peter G Tipping,Stephen R Holdsworth

The Journal of clinical investigation 113:1390-7 PubMed15146236

2004

Induction of B7-1 in podocytes is associated with nephrotic syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Jochen Reiser,Gero von Gersdorff,Martin Loos,Jun Oh,Katsuhiko Asanuma,Laura Giardino,Maria Pia Rastaldi,Novella Calvaresi,Haruko Watanabe,Karin Schwarz,Christian Faul,Matthias Kretzler,Anne Davidson,Hikaru Sugimoto,Raghu Kalluri,Arlene H Sharpe,Jordan A Kreidberg,Peter Mundel

Infection and immunity 71:4137-43 PubMed12819105

2003

Bordetella bronchiseptica persists in the nasal cavities of mice and triggers early delivery of dendritic cells in the lymph nodes draining the lower and upper respiratory tract.

Applications

Unspecified application

Species

Unspecified reactive species

Pascale Gueirard,Patrick Ave,Anne-Marie Balazuc,Sabine Thiberge,Michel Huerre,Genevieve Milon,Nicole Guiso

Journal of immunology (Baltimore, Md. : 1950) 169:3180-90 PubMed12218136

2002

CD80+Gr-1+ myeloid cells inhibit development of antifungal Th1 immunity in mice with candidiasis.

Applications

Unspecified application

Species

Unspecified reactive species

Antonella Mencacci,Claudia Montagnoli,Angela Bacci,Elio Cenci,Lucia Pitzurra,Antonio Spreca,Manfred Kopf,Arlene H Sharpe,Luigina Romani

Journal of immunology (Baltimore, Md. : 1950) 160:2706-14 PubMed9510170

1998

Nucleic acid vaccine-induced immune responses require CD28 costimulation and are regulated by CTLA4.

Applications

Unspecified application

Species

Unspecified reactive species

J H Horspool,P J Perrin,J B Woodcock,J H Cox,C L King,C H June,D M Harlan,D C St Louis,K P Lee
View all publications
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Product promise

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