AB64116

Anti-CD80 antibody

Name: Anti-CD80 antibody (ab64116) | Abcam
Brand: Abcam Limited
SKU: AB64116
Rating: 1 (1 reviews)

(1 Review)

(11 Publications )

Rabbit Polyclonal CD80 antibody. Suitable for IHC-P and reacts with Mouse samples. Cited in 11 publications.

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD80 antibody (AB64116)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD80 antibody (AB64116)

ab64116 (1 : 40) staining CD80 in paraffin-embedded mouse lymph node tissue (left panel) using an automated system (Ventana Discovery). Right-hand panel shows negative control (no primary antibody).
Using this protocol there is strong membrane staining, of activated B cells in the germinal centres. What appear to be scattered activated B cells or possibly plasma cells in the interfollicular areas of the lymph node and blood vessels.

Sections were rehydrated and antigen retrieved in CC1 Cell Conditioning Buffer using Ventana Extended Retrieval programme. Slides were blocked in 3% H₂O₂ / 4 min / 37°C and incubated with ab64116 (1 : 40 dilution / 1 hour / 37°C). Sections then blocked (4mins / 37°C) and incubated with Dako swine anti-rabbit antibody (1 : 50, 28 min / 37°C). Staining was amplified and detected by incubation with Ventana Streptavidin ABC system (16 min / 37°C) and Ventana DAB map reagent (8

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/40", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD80 also known as B7-1 is a cell surface protein with a molecular weight of approximately 60 kDa. It is expressed on antigen-presenting cells such as dendritic cells macrophages and B cells. The CD80 protein plays a mechanical role in immune response modulation by providing necessary signals for T cell activation and survival. CD80 interacts with receptors CD28 and CTLA-4 on T cells serving as a costimulatory molecule important for productive immune responses.

Biological function summary

CD80 plays a significant role in the immune system's ability to distinguish between self and non-self. It belongs to the B7 family and participates in the formation of immunological synapses between T cells and antigen-presenting cells. This interaction is essential for triggering T cell proliferation and cytokine production forming a part of the adaptive immune response. CD80's expression increases in response to pathogen recognition enhancing the immune system's efficiency.

Pathways

CD80 is integral to the regulation of T cell-mediated immune responses. It is involved in the CD28 co-stimulatory pathway which is vital for T cell activation and function. In this pathway CD80 interacts with proteins like CD28 and CTLA-4 to modulate immune activation and regulate immune tolerance. The proper function of CD80 within this pathway ensures a balanced immune reaction preventing autoimmunity while allowing effective defense against pathogens.

CD80 plays a role in autoimmune conditions and transplantation rejection. Its overexpression or dysregulation can contribute to diseases such as rheumatoid arthritis where it modifies T cell response and inflammation. Additionally CD80 is implicated in organ transplantation influencing graft rejection outcomes due to its role in modulating immune activation. Understanding CD80 interactions with CTLA-4 and CD28 offers insight into these conditions providing potential targets for therapeutic intervention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Costimulatory molecule that belongs to the immunoglobulin superfamily that plays an important role in T-lymphocyte activation. Acts as the primary auxiliary signal augmenting the MHC/TCR signal in naive T-cells together with the CD28 receptor which is constitutively expressed on the cell surface of T-cells (PubMed : 16339518). In turn, activates different signaling pathways such as NF-kappa-B or MAPK leading to the production of different cytokines. In addition, CD28/CD80 costimulatory signal stimulates glucose metabolism and ATP synthesis of T-cells by activating the PI3K/Akt signaling pathway. Acts also as a regulator of PDL1/PDCD1 interactions to limit excess engagement of PDL1 and its inhibitory role in immune responses. Expressed on B-cells, plays a critical role in regulating interactions between B-cells and T-cells in both early and late germinal center responses, which are crucial for the generation of effective humoral immune responses (PubMed : 22450810).

See full target information Cd80

Alternative Names

CD80, B7, Cd80, T-lymphocyte activation antigen CD80, Activation B7-1 antigen

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Cancer discovery 15:1410-1436 PubMed40094380

2025

Activated T Cells Break Tumor Immunosuppression by Macrophage Reeducation.

Applications

Unspecified application

Species

Unspecified reactive species

Rosa Trotta,Silvia Rivis,Shikang Zhao,Marie-Pauline Orban,Sarah Trusso Cafarello,Iris Charatsidou,Joanna Pozniak,Jonas Dehairs,Lotte Vanheer,Carlos A Pulido Vicuna,Veerle Boecxstaens,Oliver Bechter,Francesca M Bosisio,Johannes V Swinnen,Jean-Christophe Marine,Massimiliano Mazzone

Cellular and molecular gastroenterology and hepatology 14:877-904 PubMed35863741

2022

Prolonged Administration of Melatonin Ameliorates Liver Phenotypes in Cholestatic Murine Model.

Applications

Unspecified application

Species

Unspecified reactive species

Ludovica Ceci,Lixian Chen,Leonardo Baiocchi,Nan Wu,Lindsey Kennedy,Guido Carpino,Konstantina Kyritsi,Tianhao Zhou,Travis Owen,Debjyoti Kundu,Amelia Sybenga,Abdulkadir Isidan,Burcin Ekser,Antonio Franchitto,Paolo Onori,Eugenio Gaudio,Romina Mancinelli,Heather Francis,Gianfranco Alpini,Shannon Glaser

Journal of tissue engineering 12:20417314211047100 PubMed34589198

2021

Early osteoimmunomodulatory effects of magnesium-calcium-zinc alloys.

Applications

Unspecified application

Species

Unspecified reactive species

Maryam Rahmati,Sabine Stötzel,Thaqif El Khassawna,Kamila Iskhahova,D C Florian Wieland,Berit Zeller Plumhoff,Håvard Jostein Haugen

Particle and fibre toxicology 18:6 PubMed33526046

2021

Macrophage autophagy protects mice from cerium oxide nanoparticle-induced lung fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Balasubramanyam Annangi,Zhuyi Lu,Jonathan Bruniaux,Audrey Ridoux,Vanessa Marques da Silva,Delphine Vantelon,Jorge Boczkowski,Sophie Lanone

Leukemia research reports 14:100215 PubMed32695574

2020

Immune checkpoints bone marrow expression as the predictor of clinical outcome in myelodysplastic syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Nikolai Tcvetkov,Artem Gusak,Elena Morozova,Ivan Moiseev,Vadim Baykov,Maria Barabanshikova,Kirill Lepik,Evgenyi Bakin,Julia Vlasova,Anna Osipova,Ludmila Zubarovskaya,Boris Afanasyev

ACS applied bio materials 3:4335-4347 PubMed34841220

2020

Targeted Imaging of CD206 Expressing Tumor-Associated M2-like Macrophages Using Mannose-Conjugated Antibiofouling Magnetic Iron Oxide Nanoparticles.

Applications

Unspecified application

Species

Unspecified reactive species

Yuancheng Li,Hui Wu,Bing Ji,Weiping Qian,Siyuan Xia,Liya Wang,Yaolin Xu,Jing Chen,Lily Yang,Hui Mao

Cell 177:1172-1186.e14 PubMed31031009

2019

Adaptive Immune Resistance Emerges from Tumor-Initiating Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yuxuan Miao,Hanseul Yang,John Levorse,Shaopeng Yuan,Lisa Polak,Megan Sribour,Bhuvanesh Singh,Michael D Rosenblum,Elaine Fuchs

Cell reports 27:1176-1189.e5 PubMed31018132

2019

YAP Aggravates Inflammatory Bowel Disease by Regulating M1/M2 Macrophage Polarization and Gut Microbial Homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Zhou,Weiyun Li,Shuang Wang,Panli Zhang,Qiong Wang,Jun Xiao,Chi Zhang,Xin Zheng,Xiaoyan Xu,Shengjie Xue,Lijian Hui,Hongbin Ji,Bin Wei,Hongyan Wang

Molecular and cellular biology 38: PubMed29133460

2018

Elevated HuR in Pancreas Promotes a Pancreatitis-Like Inflammatory Microenvironment That Facilitates Tumor Development.

Applications

Unspecified application

Species

Unspecified reactive species

Weidan Peng,Narumi Furuuchi,Ludmila Aslanukova,Yu-Hung Huang,Samantha Z Brown,Wei Jiang,Sankar Addya,Vikalp Vishwakarma,Erika Peters,Jonathan R Brody,Dan A Dixon,Janet A Sawicki

Nature communications 7:11406 PubMed27147225

2016

miR-424(322) reverses chemoresistance via T-cell immune response activation by blocking the PD-L1 immune checkpoint.

Applications

Species

Unspecified reactive species

Shaohua Xu,Zhen Tao,Bo Hai,Huagen Liang,Ying Shi,Tao Wang,Wen Song,Yong Chen,Jun OuYang,Jinhong Chen,Fanfei Kong,Yishan Dong,Shi-Wen Jiang,Weiyong Li,Ping Wang,Zhiyong Yuan,Xiaoping Wan,Chenguang Wang,Wencheng Li,Xiaoping Zhang,Ke Chen

View all publications

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