AB314098

Anti-CD80 antibody [BLR237K] - BSA free

Recombinant
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Rabbit Recombinant Monoclonal CD80 antibody. Suitable for Flow Cyt, ICC, WB and reacts with Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Mouse Cd80 aa 1-250.

View Alternative Names

CD80, B7, Cd80, T-lymphocyte activation antigen CD80, Activation B7-1 antigen

3 Images

Flow Cytometry - Anti-CD80 antibody [BLR237K] - BSA free (AB314098)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD80 antibody [BLR237K] - BSA free (AB314098)

Detection of mouse CD80 (shaded) in CH27 cells by flow cytometry. Antibody : Rabbit anti-CD80 recombinant monoclonal [BLR237K] (A700-237) or isotype control (unshaded). Secondary : DyLight® 650-conjugated goat anti-rabbit IgG.

Supplier Data

Immunocytochemistry - Anti-CD80 antibody [BLR237K] - BSA free (AB314098)

Detection of mouse CD80 by immunocytochemistry. Sample : FFPE section of C2C12 cells. Antibody : Rabbit antimCD80 recombinant monoclonal antibody [BLR237K] (A700-237). Secondary : HRP-conjugated goat anti-rabbit IgG.

Supplier Data

Western blot - Anti-CD80 antibody [BLR237K] - BSA free (AB314098)

Samples : Whole cell lysate (50 µg) from C2C12, NIH 3T3, CH27, TCMK-1, and CT26 cells prepared using NETN lysis buffer. Antibody : Rabbit anti-CD80 recombinant monoclonal antibody [BLR237K] (A700-237) used at 1 : 1000. Secondary : HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 3 minutes. Lower Panel : Rabbit anti-Actin recombinant monoclonal antibody [BLR057F].

All lanes:

Western blot - Anti-CD80 antibody [BLR237K] - BSA free (ab314098) at 1/1000 dilution

Lane 1:

C2C12 Whole cell lysate at 50 µg

Lane 2:

NIH 3T3 Whole cell lysate at 50 µg

Lane 3:

CH27 Whole cell lysate at 50 µg

Lane 4:

TCMK-1 Whole cell lysate at 50 µg

Lane 5:

CT26 Whole cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

BLR237K

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

Flow Cyt, ICC, WB

applications

Immunogen

Recombinant Fragment Protein within Mouse Cd80 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

Q00609

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Reactivity data

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Product details

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 98% Borate buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

Up to 12 months

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD80 also known as B7-1 is a cell surface protein with a molecular weight of approximately 60 kDa. It is expressed on antigen-presenting cells such as dendritic cells macrophages and B cells. The CD80 protein plays a mechanical role in immune response modulation by providing necessary signals for T cell activation and survival. CD80 interacts with receptors CD28 and CTLA-4 on T cells serving as a costimulatory molecule important for productive immune responses.

Biological function summary

CD80 plays a significant role in the immune system's ability to distinguish between self and non-self. It belongs to the B7 family and participates in the formation of immunological synapses between T cells and antigen-presenting cells. This interaction is essential for triggering T cell proliferation and cytokine production forming a part of the adaptive immune response. CD80's expression increases in response to pathogen recognition enhancing the immune system's efficiency.

Pathways

CD80 is integral to the regulation of T cell-mediated immune responses. It is involved in the CD28 co-stimulatory pathway which is vital for T cell activation and function. In this pathway CD80 interacts with proteins like CD28 and CTLA-4 to modulate immune activation and regulate immune tolerance. The proper function of CD80 within this pathway ensures a balanced immune reaction preventing autoimmunity while allowing effective defense against pathogens.

CD80 plays a role in autoimmune conditions and transplantation rejection. Its overexpression or dysregulation can contribute to diseases such as rheumatoid arthritis where it modifies T cell response and inflammation. Additionally CD80 is implicated in organ transplantation influencing graft rejection outcomes due to its role in modulating immune activation. Understanding CD80 interactions with CTLA-4 and CD28 offers insight into these conditions providing potential targets for therapeutic intervention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Costimulatory molecule that belongs to the immunoglobulin superfamily that plays an important role in T-lymphocyte activation. Acts as the primary auxiliary signal augmenting the MHC/TCR signal in naive T-cells together with the CD28 receptor which is constitutively expressed on the cell surface of T-cells (PubMed : 16339518). In turn, activates different signaling pathways such as NF-kappa-B or MAPK leading to the production of different cytokines. In addition, CD28/CD80 costimulatory signal stimulates glucose metabolism and ATP synthesis of T-cells by activating the PI3K/Akt signaling pathway. Acts also as a regulator of PDL1/PDCD1 interactions to limit excess engagement of PDL1 and its inhibitory role in immune responses. Expressed on B-cells, plays a critical role in regulating interactions between B-cells and T-cells in both early and late germinal center responses, which are crucial for the generation of effective humoral immune responses (PubMed : 22450810).

See full target information Cd80

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD80 antibody [BLR237K] - BSA free

Flow Cytometry - Anti-CD80 antibody [BLR237K] - BSA free (AB314098)

Immunocytochemistry - Anti-CD80 antibody [BLR237K] - BSA free (AB314098)

Western blot - Anti-CD80 antibody [BLR237K] - BSA free (AB314098)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise