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AB62636

Anti-CD80 antibody [RM80]

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(1 Publication)

Rat Monoclonal CD80 antibody. Suitable for Flow Cyt, IHC-Fr and reacts with Mouse samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing Cd80 protein.

View Alternative Names

CD80, B7, Cd80, T-lymphocyte activation antigen CD80, Activation B7-1 antigen

  • 519 FITC

    FITC Anti-CD80 antibody [RM80]

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

RM80

Isotype

IgG2a

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt, IHC-Fr

applications

Immunogen

Cell preparation containing Cd80 protein. The exact immunogen used to generate this antibody is proprietary information.

Q00609

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>Use 10ul to label 1x10^6 cells in 100ul. <a href='/en-us/products/primary-antibodies/rat-igg2a-kappa-monoclonal-rtk2758-isotype-control-ab18450'>ab18450</a> - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
Storage buffer
pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD80 also known as B7-1 is a cell surface protein with a molecular weight of approximately 60 kDa. It is expressed on antigen-presenting cells such as dendritic cells macrophages and B cells. The CD80 protein plays a mechanical role in immune response modulation by providing necessary signals for T cell activation and survival. CD80 interacts with receptors CD28 and CTLA-4 on T cells serving as a costimulatory molecule important for productive immune responses.
Biological function summary

CD80 plays a significant role in the immune system's ability to distinguish between self and non-self. It belongs to the B7 family and participates in the formation of immunological synapses between T cells and antigen-presenting cells. This interaction is essential for triggering T cell proliferation and cytokine production forming a part of the adaptive immune response. CD80's expression increases in response to pathogen recognition enhancing the immune system's efficiency.

Pathways

CD80 is integral to the regulation of T cell-mediated immune responses. It is involved in the CD28 co-stimulatory pathway which is vital for T cell activation and function. In this pathway CD80 interacts with proteins like CD28 and CTLA-4 to modulate immune activation and regulate immune tolerance. The proper function of CD80 within this pathway ensures a balanced immune reaction preventing autoimmunity while allowing effective defense against pathogens.

CD80 plays a role in autoimmune conditions and transplantation rejection. Its overexpression or dysregulation can contribute to diseases such as rheumatoid arthritis where it modifies T cell response and inflammation. Additionally CD80 is implicated in organ transplantation influencing graft rejection outcomes due to its role in modulating immune activation. Understanding CD80 interactions with CTLA-4 and CD28 offers insight into these conditions providing potential targets for therapeutic intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Costimulatory molecule that belongs to the immunoglobulin superfamily that plays an important role in T-lymphocyte activation. Acts as the primary auxiliary signal augmenting the MHC/TCR signal in naive T-cells together with the CD28 receptor which is constitutively expressed on the cell surface of T-cells (PubMed : 16339518). In turn, activates different signaling pathways such as NF-kappa-B or MAPK leading to the production of different cytokines. In addition, CD28/CD80 costimulatory signal stimulates glucose metabolism and ATP synthesis of T-cells by activating the PI3K/Akt signaling pathway. Acts also as a regulator of PDL1/PDCD1 interactions to limit excess engagement of PDL1 and its inhibitory role in immune responses. Expressed on B-cells, plays a critical role in regulating interactions between B-cells and T-cells in both early and late germinal center responses, which are crucial for the generation of effective humoral immune responses (PubMed : 22450810).
See full target information Cd80
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of immunology (Baltimore, Md. : 1950) 167:5741-8 PubMed11698447

2001

Opposing effects of anti-activation-inducible lymphocyte-immunomodulatory molecule/inducible costimulator antibody on the development of acute versus chronic graft-versus-host disease.

Applications

Unspecified application

Species

Unspecified reactive species

S Ogawa,G Nagamatsu,M Watanabe,S Watanabe,T Hayashi,S Horita,K Nitta,H Nihei,K Tezuka,R Abe
View all publications
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Product promise

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