Mouse Monoclonal CD81 antibody. Carrier free. Suitable for Flow Cyt and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Cell preparation containing CD81 protein.
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![Flow Cytometry - Anti-CD81 antibody [1D6] - Low endotoxin, Azide free (AB35026), expandable thumbnail](https://content.abcam.com/products/images/cd81-antibody-1d6-low-endotoxin-azide-free-ab35026--flow-cytometry-img78654.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CD81 antibody [1D6] - Low endotoxin, Azide free (AB35026)")
Publications
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- Molecular therapy. Nucleic acids 28:17-312022Exosomal microRNAs synergistically trigger stromal fibroblasts in breast cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIolanda Scognamiglio,Lorenza Cocca,Ilaria Puoti,Francesco Palma,Francesco Ingenito,Cristina Quintavalle,Alessandra Affinito,Giuseppina Roscigno,Silvia Nuzzo,Rosario Vincenzo Chianese,Stefania Belli,Guglielmo Thomas,Timo Schomann,Alan Chan,Maria Patrizia Stoppelli,Gerolama CondorelliPubMed 35317202
- Science advances 5:eaax88492019Mechanisms of nuclear content loading to exosomes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAkira Yokoi et. al.PubMed 31799396
- Oncology reports 42:2528-25362019Exosomes isolated from CAPS1‑overexpressing colorectal cancer cells promote cell migration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBingrui Wu et. al.PubMed 31638236
- Frontiers in immunology 10:16442019TLR9 Mediated Tumor-Stroma Interactions in Human Papilloma Virus (HPV)-Positive Head and Neck Squamous Cell Carcinoma Up-Regulate PD-L1 and PD-L2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesParamita Baruah et. al.PubMed 31379843
- Toxicological sciences : an official journal of the Society of Toxicology 165:40-492018Arsenic Alters Exosome Quantity and Cargo to Mediate Stem Cell Recruitment Into a Cancer Stem Cell-Like Phenotype.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNtube N O Ngalame,Anthony L Luz,Ngome Makia,Erik J TokarPubMed 30169766
- American journal of physiology. Renal physiology 301:F443-92011Renal inflammation and elevated blood pressure in a mouse model of reduced {beta}-ENaC.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Cell preparation containing CD81 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P60033
Reactivity data
Select an application| Flow Cyt | WB | |
|---|---|---|
| Human | Tested | Not recommended |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/5.00000 - 1/10.00000 | Notes Use 10μl of the suggested working dilution to label 106 cells in 100μl. |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Although there are references supporting the use of the clone in WB, our internal testing failed to achieve suitable results. Unfortunately this antibody cannot be guaranteed for WB |
Associated Products
Select an associated product type
4 products for Alternative Product
1 product for Alternative Version
Target data
Function
Structural component of specialized membrane microdomains known as tetraspanin-enriched microdomains (TERMs), which act as platforms for receptor clustering and signaling. Essential for trafficking and compartmentalization of CD19 receptor on the surface of activated B cells (PubMed:16449649, PubMed:20237408, PubMed:27881302). Upon initial encounter with microbial pathogens, enables the assembly of CD19-CR2/CD21 and B cell receptor (BCR) complexes at signaling TERMs, lowering the threshold dose of antigen required to trigger B cell clonal expansion and antibody production (PubMed:15161911, PubMed:20237408). In T cells, facilitates the localization of CD247/CD3 zeta at antigen-induced synapses with B cells, providing for costimulation and polarization toward T helper type 2 phenotype (PubMed:22307619, PubMed:23858057, PubMed:8766544). Present in MHC class II compartments, may also play a role in antigen presentation (PubMed:8409388, PubMed:8766544). Can act both as positive and negative regulator of homotypic or heterotypic cell-cell fusion processes. Positively regulates sperm-egg fusion and may be involved in acrosome reaction (By similarity). In myoblasts, associates with CD9 and PTGFRN and inhibits myotube fusion during muscle regeneration (By similarity). In macrophages, associates with CD9 and beta-1 and beta-2 integrins, and prevents macrophage fusion into multinucleated giant cells specialized in ingesting complement-opsonized large particles (PubMed:12796480). Also prevents the fusion of mononuclear cell progenitors into osteoclasts in charge of bone resorption (By similarity). May regulate the compartmentalization of enzymatic activities. In T cells, defines the subcellular localization of dNTPase SAMHD1 and permits its degradation by the proteasome, thereby controlling intracellular dNTP levels (PubMed:28871089). Also involved in cell adhesion and motility. Positively regulates integrin-mediated adhesion of macrophages, particularly relevant for the inflammatory response in the lung (By similarity). (Microbial infection) Acts as a receptor for hepatitis C virus (HCV) in hepatocytes. Association with CLDN1 and the CLDN1-CD81 receptor complex is essential for HCV entry into host cell. (Microbial infection) Involved in SAMHD1-dependent restriction of HIV-1 replication. May support early replication of both R5- and X4-tropic HIV-1 viruses in T cells, likely via proteasome-dependent degradation of SAMHD1. (Microbial infection) Specifically required for Plasmodium falciparum infectivity of hepatocytes, controlling sporozoite entry into hepatocytes via the parasitophorous vacuole and subsequent parasite differentiation to exoerythrocytic forms.
Alternative names
CD81, TAPA1, TSPAN28, CD81 antigen, 26 kDa cell surface protein TAPA-1, Target of the antiproliferative antibody 1, Tetraspanin-28, Tspan-28
Recommended products
Mouse Monoclonal CD81 antibody. Carrier free. Suitable for Flow Cyt and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Cell preparation containing CD81 protein.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Cell preparation containing CD81 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P60033
- Carrier free
- Yes
- Clone number
- 1D6
- Purification technique
- Affinity purification Protein G
- Specificity
ab35026 recognises CD81, a 26kD cell surface antigen and a member of the tetraspanin family.
- Concentration
- Purification notes
This antibody was purified from tissue culture supernatant.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
ab35026 is a potent CD81 reagent, induces homotypic adhesion and has powerful anti proliferative effects.
Endotoxin Level: <0.01 Eu/ug (low endotoxin grade).
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD81 also known as TAPA-1 or 26 kDa protein is a member of the tetraspanin family featuring four transmembrane domains. The molecular weight of CD81 is approximately 26-28 kDa. This protein exhibits expression in numerous cell types such as leukocytes endothelial cells and epithelial tissues. CD81 plays a mechanical role by facilitating membrane protein interactions and contributing to cellular processes like adhesion and morphogenesis. Researchers have identified specific isoforms like M38 and labels like 1D6 CHAN in studies involving this target often analyzing CD81 through techniques like Western blot to determine expression levels and molecular weight.
- Biological function summary
CD81 interacts with other tetraspanins and forms complexes within the membrane to regulate cellular signaling trafficking and adhesion. It participates in the assembly of larger tetraspanin-enriched microdomains which are important for efficient signaling and functional variety. These complexes modulate cell morphology proliferation and development influencing immune responses and pathogen entry to cells. The presence of CD81 in diverse tissues implies its involvement in a wide range of cellular processes forming essential complexes with proteins like integrins that further engage in tissue repair and immunological defense.
- Pathways
CD81 plays significant roles in the immune system and viral entry pathways. It interacts with other proteins like CD9 and CD19 within the immune response pathways regulating lymphocyte activation and differentiation. CD81 is notably a coreceptor in the hepatitis C virus (HCV) entry pathway facilitating viral attachment and fusion into host cells. These interactions illustrate CD81's involvement in modulation of immune cell responses and influence on pathogen infection processes integrating into the complex cellular pathways important for maintaining homeostasis and response to external stimuli.
- Associated diseases and disorders
CD81 has connections to hepatitis C virus (HCV) infections and immunological disorders such as systemic lupus erythematosus (SLE). During HCV infection CD81 serves as an important entry point for the virus interacting with proteins like claudin-1 and scavenger receptor class B type I (SR-BI) enabling viral entry and replication within liver cells. In SLE alterations in CD81 expression can impact autoantibody production and lymphocyte behavior contributing to the disease's pathology. Understanding CD81's role in these diseases provides insight into therapeutic targets and potential interventions for managing infections and autoimmune responses.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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1 product image
![Flow Cytometry - Anti-CD81 antibody [1D6] - Low endotoxin, Azide free (ab35026), expandable thumbnail](https://content.abcam.com/products/images/cd81-antibody-1d6-low-endotoxin-azide-free-ab35026--flow-cytometry-img78654.jpg "Flow Cytometry - Anti-CD81 antibody [1D6] - Low endotoxin, Azide free (ab35026)")
Flow Cytometry - Anti-CD81 antibody [1D6] - Low endotoxin, Azide free (ab35026)
Overlay histogram showing HAP1 wildtype (green line) and HAP1-CD81 knockout cells (red line) stained with ab35026. The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab35026, 0.1μg/ml) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) presorbed (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117) at 1/2000 dilution for 30 min at 22°C.
A mouse IgG1 isotype control antibody (Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control ab170190) was used at the same concentration and conditions as the primary antibody (HAP1 wildtype - black line, HAP1-CD81 knockout - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).
Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.
This antibody can also be used in HAP1 cells fixed with 80% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 15 min under the same conditions.
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