AB219209

Anti-CD81 antibody [EPR21916]

Name: Anti-CD81 antibody [EPR21916] (ab219209) | Abcam
Brand: Abcam Limited
SKU: AB219209
Price: 180 USD
Availability: InStock
Rating: 3 (3 reviews)

20ul selling size
RabMAb
Recombinant
What is this?

(3 Reviews)

(21 Publications)

Anti-CD81 antibody [EPR21916] (ab219209) is a rabbit monoclonal antibody detecting CD81 in Flow Cytometry, IHC-P, ICC/IF. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency
- Over 10 publications

View Alternative Names

CD81, TAPA1, TSPAN28, CD81 antigen, 26 kDa cell surface protein TAPA-1, Target of the antiproliferative antibody 1, Tetraspanin-28, Tspan-28

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-CD81 antibody [EPR21916] (AB219209)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CD81 antibody [EPR21916] (AB219209)

Immunofluorescent analysis of 100% methanol-fixed Jurkat (human T cell leukemia cell line from peripheral blood) cells and HepG2 (human liver hepatocellular carcinoma cell line) cells labeling CD81 with ab219209 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing membranous staining in Jurkat cells.

Negative control : HepG2 (PMID : 16641285).

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD81 antibody [EPR21916] (AB219209)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD81 antibody [EPR21916] (AB219209)

Immunohistochemical analysis of paraffin-embedded human diffuse large B cell lymphoma tissue labeling CD81 with ab219209 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on tumor cells of human diffuse large B cell lymphoma (PMID : 20004001). Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD81 antibody [EPR21916] (AB219209)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CD81 with ab219209 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining in the germinal center of human tonsil (PMID : 20004001). Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD81 antibody [EPR21916] (AB219209)

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling CD81 with ab219209 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Mainly membranous staining in human liver (PMID : 19489072). Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Flow Cytometry - Anti-CD81 antibody [EPR21916] (AB219209)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD81 antibody [EPR21916] (AB219209)

Flow cytometric analysis of Jurkat (human T cell leukemia cell line from peripheral blood) cells and HepG2 (human liver hepatocellular carcinoma cell line) cell lines labeling CD81 with ab219209 at 1/600 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells incubated with secondary antibody only) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

Gated on viable cells.

Negative control : HepG2 (PMID : 16641285).

Carrier free

Anti-CD81 antibody [EPR21916] - BSA and Azide free
660 APC

APC Anti-CD81 antibody [EPR21916]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-CD81 antibody [EPR21916]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-CD81 antibody [EPR21916]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-CD81 antibody [EPR21916]
578 PE

PE Anti-CD81 antibody [EPR21916]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR21916

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt, IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/600", "FlowCyt-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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Product details

What is this antibody validated in?
Anti-CD81 antibody [EPR21916] (ab219209) is a rabbit recombinant monoclonal antibody and is validated for use in Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

Trusted by the scientific community
Anti-CD81 [EPR21916] (ab219209) was first used in a scientific publication in 2018 and has been cited over 10 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR21916] also available for your convenience: ab219209, Carrier free - ab233692, PE - ab283757, Alexa Fluor® 594 - ab286809, Alexa Fluor® 488 - ab302976, Alexa Fluor® 647 - ab303459, APC - ab314278

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD81 also known as TAPA-1 or 26 kDa protein is a member of the tetraspanin family featuring four transmembrane domains. The molecular weight of CD81 is approximately 26-28 kDa. This protein exhibits expression in numerous cell types such as leukocytes endothelial cells and epithelial tissues. CD81 plays a mechanical role by facilitating membrane protein interactions and contributing to cellular processes like adhesion and morphogenesis. Researchers have identified specific isoforms like M38 and labels like 1D6 CHAN in studies involving this target often analyzing CD81 through techniques like Western blot to determine expression levels and molecular weight.

Biological function summary

CD81 interacts with other tetraspanins and forms complexes within the membrane to regulate cellular signaling trafficking and adhesion. It participates in the assembly of larger tetraspanin-enriched microdomains which are important for efficient signaling and functional variety. These complexes modulate cell morphology proliferation and development influencing immune responses and pathogen entry to cells. The presence of CD81 in diverse tissues implies its involvement in a wide range of cellular processes forming essential complexes with proteins like integrins that further engage in tissue repair and immunological defense.

Pathways

CD81 plays significant roles in the immune system and viral entry pathways. It interacts with other proteins like CD9 and CD19 within the immune response pathways regulating lymphocyte activation and differentiation. CD81 is notably a coreceptor in the hepatitis C virus (HCV) entry pathway facilitating viral attachment and fusion into host cells. These interactions illustrate CD81's involvement in modulation of immune cell responses and influence on pathogen infection processes integrating into the complex cellular pathways important for maintaining homeostasis and response to external stimuli.

CD81 has connections to hepatitis C virus (HCV) infections and immunological disorders such as systemic lupus erythematosus (SLE). During HCV infection CD81 serves as an important entry point for the virus interacting with proteins like claudin-1 and scavenger receptor class B type I (SR-BI) enabling viral entry and replication within liver cells. In SLE alterations in CD81 expression can impact autoantibody production and lymphocyte behavior contributing to the disease's pathology. Understanding CD81's role in these diseases provides insight into therapeutic targets and potential interventions for managing infections and autoimmune responses.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Structural component of specialized membrane microdomains known as tetraspanin-enriched microdomains (TERMs), which act as platforms for receptor clustering and signaling. Essential for trafficking and compartmentalization of CD19 receptor on the surface of activated B cells (PubMed : 16449649, PubMed : 20237408, PubMed : 27881302). Upon initial encounter with microbial pathogens, enables the assembly of CD19-CR2/CD21 and B cell receptor (BCR) complexes at signaling TERMs, lowering the threshold dose of antigen required to trigger B cell clonal expansion and antibody production (PubMed : 15161911, PubMed : 20237408). In T cells, facilitates the localization of CD247/CD3 zeta at antigen-induced synapses with B cells, providing for costimulation and polarization toward T helper type 2 phenotype (PubMed : 22307619, PubMed : 23858057, PubMed : 8766544). Present in MHC class II compartments, may also play a role in antigen presentation (PubMed : 8409388, PubMed : 8766544). Can act both as positive and negative regulator of homotypic or heterotypic cell-cell fusion processes. Positively regulates sperm-egg fusion and may be involved in acrosome reaction (By similarity). In myoblasts, associates with CD9 and PTGFRN and inhibits myotube fusion during muscle regeneration (By similarity). In macrophages, associates with CD9 and beta-1 and beta-2 integrins, and prevents macrophage fusion into multinucleated giant cells specialized in ingesting complement-opsonized large particles (PubMed : 12796480). Also prevents the fusion of mononuclear cell progenitors into osteoclasts in charge of bone resorption (By similarity). May regulate the compartmentalization of enzymatic activities. In T cells, defines the subcellular localization of dNTPase SAMHD1 and permits its degradation by the proteasome, thereby controlling intracellular dNTP levels (PubMed : 28871089). Also involved in cell adhesion and motility. Positively regulates integrin-mediated adhesion of macrophages, particularly relevant for the inflammatory response in the lung (By similarity).. (Microbial infection) Acts as a receptor for hepatitis C virus (HCV) in hepatocytes. Association with CLDN1 and the CLDN1-CD81 receptor complex is essential for HCV entry into host cell.. (Microbial infection) Involved in SAMHD1-dependent restriction of HIV-1 replication. May support early replication of both R5- and X4-tropic HIV-1 viruses in T cells, likely via proteasome-dependent degradation of SAMHD1.. (Microbial infection) Specifically required for Plasmodium falciparum infectivity of hepatocytes, controlling sporozoite entry into hepatocytes via the parasitophorous vacuole and subsequent parasite differentiation to exoerythrocytic forms.

See full target information CD81

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Publications (21)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:13797 PubMed40258894

2025

Exploring the mechanism of bone marrow mesenchymal stromal cell exosomes in respiratory syncytial virus infection based on miRNA sequencing.

Applications

Unspecified application

Species

Unspecified reactive species

Bing Yao,Jinglei Liu,Zexiang Li,Jing Xie,Yinhe Luo,Mengqing Wang

Journal of nanobiotechnology 23:51 PubMed39875954

2025

Peripheral nerves modulate the peri-implant osteogenesis under type 2 diabetes through exosomes derived from schwann cells via miR-15b-5p/Txnip signaling axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yingying Wang,Fang Qu,Yaqin Wu,Kengliang Lan,Yingyi Shen,Ziang Wu,Qi Zhong,Ximeng Cao,Zhen Fan,Chun Xu

Journal of lipid research 65:100657 PubMed39326787

2024

sEV-mediated lipid droplets transferred from bone marrow adipocytes promote ferroptosis and impair osteoblast function.

Applications

Unspecified application

Species

Unspecified reactive species

Weibo Huang,Feng Hua,Tong Su,Chenghao Zhou,Kangcheng Zhao,Dianwen Song

Bioactive materials 37:1-13 PubMed38515611

2024

Thread-structural microneedles loaded with engineered exosomes for annulus fibrosus repair by regulating mitophagy recovery and extracellular matrix homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Shaojun Hu,Meng Zhu,Hongyuan Xing,Yucheng Xue,Jun Li,Zhan Wang,Zhou Zhu,Miaojie Fang,Zilong Li,Jianbin Xu,Yong He,Ning Zhang

Acta neuropathologica communications 12:35 PubMed38414005

2024

Decoding of the surfaceome and endocytome in primary glioblastoma cells identifies potential target antigens in the hypoxic tumor niche.

Applications

Unspecified application

Species

Unspecified reactive species

Kelin Gonçalves de Oliveira,Anna Bång-Rudenstam,Sarah Beyer,Axel Boukredine,Hugo Talbot,Valeria Governa,Maria C Johansson,Ann-Sofie Månsson,Karin Forsberg-Nilsson,Johan Bengzon,Johan Malmström,Charlotte Welinder,Mattias Belting

Cell transplantation 32:9636897231219830 PubMed38102784

2023

Extracellular Vesicles Derived From 3D Cultured Antler Stem Cells Serve as a New Drug Vehicle in Osteosarcoma Treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Pengfei Hu,Jinchi Ying,Yusu Wang,Tiantian Jiang,Zheng Pan,Chen Zhao,Jiping Li,Chunyi Li

Organogenesis 19:2285836 PubMed38031805

2023

Regulation of Interferon-β-Modified Human Umbilical Cord Mesenchymal Stem Cell-Derived Exosomes in Proliferation and Apoptosis of Prostate Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Haichao Yuan,Senmao Li,Zhengping Zhao,Yan Wang

European journal of histochemistry : EJH 67: PubMed37503653

2023

Therapeutic effects of human umbilical cord mesenchymal stem cell-derived extracellular vesicles on ovarian functions through the PI3K/Akt cascade in mice with premature ovarian failure.

Applications

Unspecified application

Species

Unspecified reactive species

Nan Li,Xue Fan,Lihong Liu,Yanbing Liu

International journal of medical sciences 20:542-550 PubMed37057214

2023

Capsule-epithelium-fibre unit ultrastructure in the human lens.

Applications

Unspecified application

Species

Unspecified reactive species

Qingwen Yang,Lingying Ye,Jiasheng Liu,Lei Lin,Wenjing Wang,Mengchao Zhu,Lu Zhang,Jiayi Xiao,Jun Zhang,Jin Li

International journal of oncology 62: PubMed36799165

2023

Zinc finger protein‑like 1 is a novel neuroendocrine biomarker for prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Neshat Masud,Afaf Aldahish,Kenneth A Iczkowski,Ajay Kale,Girish V Shah

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD81 antibody [EPR21916]

Immunocytochemistry/ Immunofluorescence - Anti-CD81 antibody [EPR21916] (AB219209)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD81 antibody [EPR21916] (AB219209)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD81 antibody [EPR21916] (AB219209)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD81 antibody [EPR21916] (AB219209)

Flow Cytometry - Anti-CD81 antibody [EPR21916] (AB219209)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (21)

Product promise