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Anti-CD81 antibody EPR4244 is a rabbit monoclonal antibody that is used to detect CD81 in Western blot, IHC, ICC/IF, Flow cytometry, IP. Suitable for Human, Mouse, Rat samples.

- Recombinant format for unrivaled batch-batch consistency
- Antibody clone EPR4244 is the most widely used clone for CD81 on the market
- Cited in over 250 publications


Images

Western blot - Anti-CD81 antibody [EPR4244] (AB109201), expandable thumbnail
  • Western blot - Anti-CD81 antibody [EPR4244] (AB109201), expandable thumbnail
  • Western blot - Anti-CD81 antibody [EPR4244] (AB109201), expandable thumbnail
  • Western blot - Anti-CD81 antibody [EPR4244] (AB109201), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Abcam Recommends

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBIHC-PICC/IF
Human
Not recommended
Not recommended
Tested
Not recommended
Not recommended
Mouse
Not recommended
Not recommended
Tested
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Tested
Not recommended
Not recommended

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Species
Rat
Dilution info
-
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Species
Human
Dilution info
-
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Tested
Tested

Species
Mouse
Dilution info
1/1000 - 1/10000
Notes

For unpurified use at 1/500 - 1/1000.

For WB related protocols, please refer to the Protocols section below.

Species
Rat
Dilution info
1/1000 - 1/10000
Notes

For unpurified use at 1/500 - 1/1000.

For WB related protocols, please refer to the Protocols section below.

Species
Human
Dilution info
1/1000 - 1/10000
Notes

For unpurified use at 1/500 - 1/1000.

For WB related protocols, please refer to the Protocols section below.

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

13 products for Alternative Product

Target data

Function

Structural component of specialized membrane microdomains known as tetraspanin-enriched microdomains (TERMs), which act as platforms for receptor clustering and signaling. Essential for trafficking and compartmentalization of CD19 receptor on the surface of activated B cells (PubMed:16449649, PubMed:20237408, PubMed:27881302). Upon initial encounter with microbial pathogens, enables the assembly of CD19-CR2/CD21 and B cell receptor (BCR) complexes at signaling TERMs, lowering the threshold dose of antigen required to trigger B cell clonal expansion and antibody production (PubMed:15161911, PubMed:20237408). In T cells, facilitates the localization of CD247/CD3 zeta at antigen-induced synapses with B cells, providing for costimulation and polarization toward T helper type 2 phenotype (PubMed:22307619, PubMed:23858057, PubMed:8766544). Present in MHC class II compartments, may also play a role in antigen presentation (PubMed:8409388, PubMed:8766544). Can act both as positive and negative regulator of homotypic or heterotypic cell-cell fusion processes. Positively regulates sperm-egg fusion and may be involved in acrosome reaction (By similarity). In myoblasts, associates with CD9 and PTGFRN and inhibits myotube fusion during muscle regeneration (By similarity). In macrophages, associates with CD9 and beta-1 and beta-2 integrins, and prevents macrophage fusion into multinucleated giant cells specialized in ingesting complement-opsonized large particles (PubMed:12796480). Also prevents the fusion of mononuclear cell progenitors into osteoclasts in charge of bone resorption (By similarity). May regulate the compartmentalization of enzymatic activities. In T cells, defines the subcellular localization of dNTPase SAMHD1 and permits its degradation by the proteasome, thereby controlling intracellular dNTP levels (PubMed:28871089). Also involved in cell adhesion and motility. Positively regulates integrin-mediated adhesion of macrophages, particularly relevant for the inflammatory response in the lung (By similarity). (Microbial infection) Acts as a receptor for hepatitis C virus (HCV) in hepatocytes. Association with CLDN1 and the CLDN1-CD81 receptor complex is essential for HCV entry into host cell. (Microbial infection) Involved in SAMHD1-dependent restriction of HIV-1 replication. May support early replication of both R5- and X4-tropic HIV-1 viruses in T cells, likely via proteasome-dependent degradation of SAMHD1. (Microbial infection) Specifically required for Plasmodium falciparum infectivity of hepatocytes, controlling sporozoite entry into hepatocytes via the parasitophorous vacuole and subsequent parasite differentiation to exoerythrocytic forms.

Alternative names

CD81, TAPA1, TSPAN28, CD81 antigen, 26 kDa cell surface protein TAPA-1, Target of the antiproliferative antibody 1, Tetraspanin-28, Tspan-28

Recommended products

Anti-CD81 antibody EPR4244 is a rabbit monoclonal antibody that is used to detect CD81 in Western blot, IHC, ICC/IF, Flow cytometry, IP. Suitable for Human, Mouse, Rat samples.

- Recombinant format for unrivaled batch-batch consistency
- Antibody clone EPR4244 is the most widely used clone for CD81 on the market
- Cited in over 250 publications

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR4244
Purification technique
Affinity purification Protein A
Specificity

To detect stronger signal in exosome lysate with this antibody, please try protocols of exosome isolation in PMID: 33184263, PMID: 34502537 , PMID: 32121214, PMID: 32375124.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Stable for 12 months at -20°C

Notes

Product Specifications

Anti-CD81 antibody [EPR4244] (ab109201) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in WB in human, mouse, rat samples.
Anti-CD81 antibody [EPR4244] (ab109201) specifically detects CD81 (UniProt ID: P60033; Molecular weight: 26kDa) and is sold in 100 µL and 1 mL selling sizes.

Quality and Validation

Abcam's high quality manufacturing and validation processes ensure Anti-CD81 antibody [EPR4244] (ab109201) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-CD81 antibody [EPR4244] (ab109201) has been cited over 257 times in peer reviewed journals and is trusted by the scientific community.
Anti-CD81 antibody [EPR4244] (ab109201) has 6 independent reviews from customers.

Related Products
Conjugation-ready, carrier free format available for antibody clone EPR4244 - Anti-CD81 antibody [EPR4244] - BSA and Azide free ab232390.
Target Information
CD81 or TAPA1 is a tetraspanin protein that plays a significant role in oncology, particularly in the context of cancer cell proliferation and metastasis. CD81 or TAPA1 is highly expressed in several types of cancer cells and it is involved in various cellular processes, including cell adhesion, migration, and signal transduction. CD81 also contributes to the tumor microenvironment by facilitating interactions between cancer cells and their surroundings.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CD81 also known as TAPA-1 or 26 kDa protein is a member of the tetraspanin family featuring four transmembrane domains. The molecular weight of CD81 is approximately 26-28 kDa. This protein exhibits expression in numerous cell types such as leukocytes endothelial cells and epithelial tissues. CD81 plays a mechanical role by facilitating membrane protein interactions and contributing to cellular processes like adhesion and morphogenesis. Researchers have identified specific isoforms like M38 and labels like 1D6 CHAN in studies involving this target often analyzing CD81 through techniques like Western blot to determine expression levels and molecular weight.

Biological function summary

CD81 interacts with other tetraspanins and forms complexes within the membrane to regulate cellular signaling trafficking and adhesion. It participates in the assembly of larger tetraspanin-enriched microdomains which are important for efficient signaling and functional variety. These complexes modulate cell morphology proliferation and development influencing immune responses and pathogen entry to cells. The presence of CD81 in diverse tissues implies its involvement in a wide range of cellular processes forming essential complexes with proteins like integrins that further engage in tissue repair and immunological defense.

Pathways

CD81 plays significant roles in the immune system and viral entry pathways. It interacts with other proteins like CD9 and CD19 within the immune response pathways regulating lymphocyte activation and differentiation. CD81 is notably a coreceptor in the hepatitis C virus (HCV) entry pathway facilitating viral attachment and fusion into host cells. These interactions illustrate CD81's involvement in modulation of immune cell responses and influence on pathogen infection processes integrating into the complex cellular pathways important for maintaining homeostasis and response to external stimuli.

Associated diseases and disorders

CD81 has connections to hepatitis C virus (HCV) infections and immunological disorders such as systemic lupus erythematosus (SLE). During HCV infection CD81 serves as an important entry point for the virus interacting with proteins like claudin-1 and scavenger receptor class B type I (SR-BI) enabling viral entry and replication within liver cells. In SLE alterations in CD81 expression can impact autoantibody production and lymphocyte behavior contributing to the disease's pathology. Understanding CD81's role in these diseases provides insight into therapeutic targets and potential interventions for managing infections and autoimmune responses.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-CD81 antibody [EPR4244] (ab109201), expandable thumbnail

    Western blot - Anti-CD81 antibody [EPR4244] (ab109201)

    All lanes: Western blot - Anti-CD81 antibody [EPR4244] (ab109201) at 1/700 dilution

    Lane 1: Ramos (Human Burkitt's lymphoma cell line) cell lysate at 10 µg

    Lane 2: U87-MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate at 10 µg

    Lane 3: PC-12 (Rat adrenal gland pheochromocytoma cell line) cell lysate at 10 µg

    Lane 4: RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) cell lysate at 10 µg

    Secondary

    All lanes: Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 25 kDa

    Observed band size: 20 kDa

  • Western blot - Anti-CD81 antibody [EPR4244] (ab109201), expandable thumbnail

    Western blot - Anti-CD81 antibody [EPR4244] (ab109201)

    CD81 Western blot staining using rabbit Anti-CD81 antibody

    All lanes: Western blot - Anti-CD81 antibody [EPR4244] (ab109201) at 1/1000 dilution

    Lane 1: RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

    Lane 2: C2C12 (Mouse myoblasts myoblast) whole cell lysate at 20 µg

    Lane 3: Mouse brain tissue lysate at 20 µg

    Lane 4: Mouse heart tissue lysate at 20 µg

    Lane 5: Huh7 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 6: HL-60 (Human acute promyelocytic leukemia promyeloblast) whole cell lysate at 20 µg

    Lane 7: HCT 116 (Human colorectal carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 8: LNCaP (Human prostate carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 9: U-87 MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysate at 20 µg

    Lane 10: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 11: A549 (Human lung carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 12: 293T (Human embryonic kidney epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 25 kDa

    Observed band size: 25 kDa

    Exposure time: 60s

  • Western blot - Anti-CD81 antibody [EPR4244] (ab109201), expandable thumbnail

    Western blot - Anti-CD81 antibody [EPR4244] (ab109201)

    All lanes: Western blot - Anti-CD81 antibody [EPR4244] (ab109201) at 1/2000 dilution

    Lane 1: Ramos cell lysate at 10 µg

    Lane 2: U87-MG cell lysate at 10 µg

    Lane 3: PC-12 cell lysate at 10 µg

    Lane 4: Raw264.7 cell lysate at 10 µg

    Secondary

    All lanes: Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 25 kDa

    Observed band size: 20 kDa

  • Western blot - Anti-CD81 antibody [EPR4244] (ab109201), expandable thumbnail

    Western blot - Anti-CD81 antibody [EPR4244] (ab109201)

    All lanes: Western blot - Anti-CD81 antibody [EPR4244] (ab109201) at 1/1000 dilution

    Lane 1: Ramos cell lysate at 10 µg

    Lane 2: JAR cell lysate at 10 µg

    Lane 3: U87-MG cell lysate at 10 µg

    Lane 4: IM-9 cell lysate at 10 µg

    Lane 5: Peripheral blood mononuclear cell (PBMC) cell lysate at 10 µg

    Lane 6: C6 cell lysate at 10 µg

    Lane 7: PC-12 cell lysate at 10 µg

    Lane 8: RAW264.7 cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab6721)

    Predicted band size: 25 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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