Anti-CD81 antibody [M38]

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD81 antibody [M38] (AB79559)

IHC image of CD81 staining in Human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab79559, 5 μg/mL, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD81 antibody [M38] (AB79559)

Flow cytometry analysis using human peripheral blood cells with ab79559 at 4 μg/ml.

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD81 antibody [M38] (AB79559)

Separation of human lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis of human peripheral whole blood stained using ab79559 at 4 μg/ml.

• Flow Cyt

Lab

Flow Cytometry - Anti-CD81 antibody [M38] (AB79559)

Flow cytometry overlay histogram showing left Jurkat positive cells and right negative HepG2 stained with ab79559 (red line). The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interactionfollowed by the antibody (ab79559) (1x 10⁶ in 100μl at 0.2 μg/ml (1/5000)) for 30min on ice.

The secondary antibody Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed was incubated at 1/4000 for 30min on ice

Isotype control antibody (black line) was Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.

• Flow Cyt

Lab

Flow Cytometry - Anti-CD81 antibody [M38] (AB79559)

Flow cytometry overlay histogram showing wild-type HAP1 (green line) and CD81 knockout HAP1 stained with ab79559 (magenta line). The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab79559) (1x 10⁶ in 100μl at 0.2 μg/ml (1/5000)) for 30min on ice.

The secondary antibody Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed was incubated at 1/4000 for 30min on ice

Isotype control antibody Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control was used at the same concentration and conditions as the primary antibody (wild-type HAP1 - black line, CD81 knockout HAP1 - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).

Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.

• WB

Supplier Data

Western blot - Anti-CD81 antibody [M38] (AB79559)

All lanes:

Western blot - Anti-CD81 antibody [M38] (ab79559) at 2 µg/mL

All lanes:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

Secondary

All lanes:

IRDye800-conjugated Anti-Mouse Secondary Antibody

Predicted band size: 25 kDa

Observed band size: 25 kDa

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