Anti-CD81 antibody [TS81] - BSA and Azide free

• Flow Cyt

Unknown

Flow Cytometry - Anti-CD81 antibody [TS81] - BSA and Azide free (AB59477)

Overlay histogram showing Jurkat cells stained with ab59477 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab59477, 0.5μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

• Flow Cyt

Lab

Flow Cytometry - Anti-CD81 antibody [TS81] - BSA and Azide free (AB59477)

Overlay histogram showing HAP1 wildtype (green line) and HAP1-CD81 knockout cells (red line) stained with ab59477. The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab59477, 1μg/ml) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) presorbed (ab150117) at 1/2000 dilution for 30 min at 22°C.

A mouse IgG1 isotype control antibody (ab170190) was used at the same concentration and conditions as the primary antibody (HAP1 wildtype - black line, HAP1-CD81 knockout - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).

Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.

This antibody can also be used in HAP1 cells fixed with 80% methanol (5 min), , permeabilized with 0.1% PBS-Triton X-100 for 15 min under the same conditions.

• WB

CiteAb

Western blot - Anti-CD81 antibody [TS81] - BSA and Azide free (AB59477)

CD81 western blot using anti-CD81 antibody [TS81] - BSA and Azide free ab59477. Publication image and figure legend from Liu, X., Wang, S., et al., 2018, Stem Cell Res Ther, PubMed 29895333.

ab59477 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab59477 please see the product overview.

Isolation and characterization of adMSC-Exos. a Electron micrograph of exosomes isolated from adMSC-conditioned medium. The arrowheads indicate exosomes. Original scale bars = 0.5 μm. b Western blot was performed with adMSC-Exos (Exos) or adMSC-conditioned medium (Medium). CD63, HSP70 and CD81 expression levels in exosomes were detected. c Fibroblasts were incubated with Dil-labeled exosomes (Dil-Exos; Dil is shown in red) or carrier control (CON), and nuclei were stained with Hoechst 33,342 (blue). Original scale bars = 50 μm

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