Anti-CD82 antibody

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD82 antibody (AB66400)

ab66400 (1 : 80) staining CD82 in paraffin-embedded human tonsil tissue (left panel) using an automated system (Ventana Discovery). Right hand panel shows negative control (no primary antibody).
Using this protocol there is strong membrane staining of follicular dendritic cells, and the FDC network forming the structure of germinal centres is clearly highlighted by this antibody. There is associated weak staining of smaller cells in the interfollicar areas which may be T cells.

Sections were rehydrated and antigen retrieved in CC1 Cell Conditioning Buffer using Ventana Standard Retrieval programme. Slides were blocked in 3% H₂O₂ / 4 min / 37°C and incubated with ab66400 (1 : 80 dilution / 1 hour / 37°C). Sections then blocked (4mins / 37°C) and incubated with Dako swine anti-rabbit antibody (1 : 50, 28 min / 37°C). Staining was amplified and detected by incubation with Ventana Streptavidin ABC system (16 min / 37°

• IHC-P

AbReview17988****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD82 antibody (AB66400)

ab66400 (1/50) staining CD82 in paraffin-embedded Mouse salivary gland tissue. Tissue underwent fixation in formaldehyde, peroxidase blocking, protein blocking and heat mediated antigen retrieval. The secondary antibody was goat anti rabbit/mouse conjugated to HRP. For further experimental details please refer to abreview.

This image is courtesy of an abreview submitted by Antibody Solutions Ltd.

• WB

Project

Western blot - Anti-CD82 antibody (AB66400)

CD82 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

All lanes:

Western blot - Anti-CD82 antibody (ab66400) at 1 µg/mL

All lanes:

Human brain tissue lysate - total protein (<a href='/en-us/products/unavailable/human-brain-tissue-lysate-total-protein-ab29466'>ab29466</a>) at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 29 kDa

Observed band size: 34 kDa

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Exposure time: 2min

• WB

CiteAb

Western blot - Anti-CD82 antibody (AB66400)

CD82 western blot using anti-CD82 antibody ab66400. Publication image and figure legend from Zhang, Q., Tan, D., et al., 2012, PLoS One, PubMed 22679510.

ab66400 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab66400 please see the product overview.

Expression of CD82 in human placental villi and cell lines.(A) Immunostaining of CD82 in normal human placental villi in the first trimester, maternal decidua, second trimester and third trimester. (a) CD82 was strongly expressed in trophoblast columns (TC) and moderate in CTB of human placental villi during the first trimester. (c) CD82 was highly expressed in decidual cells (DC). (e) CD82 was highly expressed in the maternal decidua, not detected in EVT and very faint in anchoring villous during the early second trimester. Boxed region is enlarged on the upper panel. (g) CD82 was absent in villous and very faint in EVT during the late second trimester. (i) CD82 was moderate expressed in cytotrophoblast cells invaded into the maternal decidua and highly expressed in syncytiotrophoblast in the third trimester. (b, f, h, j) Immunohistochemical staining with anti-cytokeratin7 (CK7) as a marker of CTB, TC in the first trimester placental vill, EVT in the maternal decidua; (d) Immunohistochemical staining with anti-vimentin as a maker of decidual cells. (k, l, m, n) negative controls (NEG) on sections in which normal IgG was used in place of primary antibody. CTB : cytotrophoblast; STB : syncytiotrophoblast; TC : trophoblast column; EVT : extravillous trophoblast; MD : maternal decidua, AV : anchoring villous W : weeks of pregnancy; Bar represents 100 µm. (B) Expression of CD82 in different trophoblast cell lines determined by semiquantitative RT-PCR and Western blot, respectively. GAPDH was used as an internal control for RT-PCR or loading control for Western blot. HTR8/SVneo : a human invasive extravillous trophoblast cell line derived from immortalized first trimester trophoblast; B6Tert : immortalized cytotrophoblast cell line; JEG-3 : human choriocarcinoma cell lines. (C) Immunofluorescence of CD82 in HTR8/SVneo cell lines. Fluorescence signals specific to CD82 antibody were visualized as green, and the nuclei were shown by DAPI staining (blue).

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