AB47153

Anti-CD82 antibody [B-L2] - BSA and Azide free

Name: Anti-CD82 antibody [B-L2] - BSA and Azide free (ab47153) | Abcam
Brand: Abcam Limited
SKU: AB47153
Rating: 5 (1 reviews)

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What is this?

(1 Review)

(3 Publications)

Mouse Monoclonal CD82 antibody. Carrier free. Suitable for IHC-P, Flow Cyt and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Cell preparation containing CD82 protein.

View Alternative Names

CD82, KAI1, SAR2, ST6, TSPAN27, CD82 antigen, C33 antigen, IA4, Inducible membrane protein R2, Metastasis suppressor Kangai-1, Suppressor of tumorigenicity 6 protein, Tetraspanin-27, Tspan-27

2 Images

Flow Cytometry - Anti-CD82 antibody [B-L2] - BSA and Azide free (AB47153)

Flow Cyt

Unknown

Flow Cytometry - Anti-CD82 antibody [B-L2] - BSA and Azide free (AB47153)

A typical staining pattern with the B-L2 monoclonal antibody of lymphocytes

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD82 antibody [B-L2] - BSA and Azide free (AB47153)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD82 antibody [B-L2] - BSA and Azide free (AB47153)

IHC image of ab47153 staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab47153, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

B-L2

Isotype

IgG1

Light chain type

kappa

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt, IHC-P

applications

Immunogen

Cell preparation containing CD82 protein. The exact immunogen used to generate this antibody is proprietary information.

P27701

Specificity

Recognises the CD82 antigen, a 60 kDa protein. Cross reacts with mouse CD82 protein in cytometry.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody." } } }

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Properties and storage information

Form

Liquid

Purification technique

Ion exchange chromatography

Purification notes

Sterile-filtered through 0.22 µm and treated to remove endotoxins. Carrier and preservative free.

Storage buffer

pH: 7.3 - 7.5 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD82 also known as KAI1 is a member of the tetraspanin family of proteins. It has a molecular mass of approximately 29 to 30 kDa. This protein is widely expressed on the surface of various cell types such as epithelial cells lymphocytes and endothelial cells. It features four transmembrane domains implying a role in organizing membrane microdomains. CD82 is known for its role in promoting cell adhesion and motility through its involvement in protein-protein interactions on the cell surface.

Biological function summary

CD82 plays a significant role in regulating cell signaling and communication during tumor progression and metastasis. It participates in the formation of tetraspanin-enriched microdomains by associating with other tetraspanins and integrins influencing the cellular microenvironment. Additionally CD82 impacts immune cell interactions by modulating activities of T cells and B cells. Despite not being the predominant component in any major protein complex CD82 influences many cellular processes by regulating diverse signaling pathways.

Pathways

CD82 modulates both the PI3K-Akt signaling pathway and the Wnt signaling pathway. These pathways are critical in controlling cellular processes like growth and survival contributing to the metastasis-suppressive effects of CD82. In these contexts CD82 associates with proteins such as integrins and cadherins which facilitate its regulation of signal transduction and cellular dynamics. CD82's role in these pathways shows its importance in controlling cancer cell behavior and immune system interactions.

CD82 has been linked to various cancers particularly prostate cancer and melanoma. Its downregulation or loss often correlates with tumor progression and metastasis indicating its function as a metastasis suppressor. In cancer CD82 interacts with proteins like epidermal growth factor receptor (EGFR) and other integrins affecting disease progression by altering cell adhesion migration and invasion. CD82’s expression level serves as a potential biomarker for cancer prognosis and treatment outcomes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Structural component of specialized membrane microdomains known as tetraspanin-enriched microdomains (TERMs), which act as platforms for receptor clustering and signaling (PubMed : 19497983). Participates thereby in diverse biological functions such as cell signal transduction, adhesion, migration and protein trafficking. Acts as a attenuator of EGF signaling, facilitating ligand-induced endocytosis of the receptor and its subsequent desensitization (PubMed : 10985391, PubMed : 35538033). Mechanistically, modulates ligand-induced ubiquitination and trafficking of EGFR via E3 ligase CBL phosphorylation by PKC (PubMed : 23897813). Increases cell-matrix adhesion by regulating the membrane organization of integrin alpha4/ITA4 (PubMed : 24623721, PubMed : 8757325). Modulates adhesion and suppresses cell migration through other integrins such as the alpha6/ITGA6 and beta1/ITGB1 (PubMed : 15557282, PubMed : 17560548). Decreases cell-associated plasminogen activation by interfering with the interaction between urokinase-type plasminogen activator/PLAU and its receptor PLAUR (PubMed : 15677461). Associates with CD4 or CD8 and delivers costimulatory signals for the TCR/CD3 pathway. Plays a role in TLR9 trafficking to acidified CpG-containing compartments by controlling interaction between TLR9 and VAMP3 and subsequent myddosome assembly (By similarity). Inhibits LPS-induced inflammatory response by preventing binding of LPS to TLR4 on the cell surface (PubMed : 36945827). Plays a role in the activation of macrophages into anti-inflammatory phenotypes (By similarity). Independently of Toll-like receptor (TLR) signaling, is recruited to pathogen-containing phagosomes prior to fusion with lysosomes and thereby participates in antigen presentation (By similarity). Also acts to control angiogenesis and switch angiogenic milieu to quiescent state by binding and sequestering VEGFA and PDGFB to inhibit the signaling they trigger via their respective cell surface receptor (PubMed : 34530889).

See full target information CD82

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Oncogene 36:4381-4392 PubMed28368419

2017

ΔNp63α promotes adhesion of metastatic prostate cancer cells to the bone through regulation of CD82.

Applications

Flow Cyt

Species

Unspecified reactive species

V Di Giacomo,T V Tian,A Mas,M Pecoraro,L Batlle-Morera,L Noya,J Martín-Caballero,J Ruberte,W M Keyes

Oncogene 35:4132-40 PubMed26592446

2015

Tetraspanin CD82 regulates bone marrow homing of acute myeloid leukemia by modulating the molecular organization of N-cadherin.

Applications

ICC/IF

Species

Unspecified reactive species

K D Marjon,C M Termini,K L Karlen,C Saito-Reis,C E Soria,K A Lidke,J M Gillette

Infection and immunity 79:1098-106 PubMed21149584

2010

The tetraspanin CD82 is specifically recruited to fungal and bacterial phagosomes prior to acidification.

Applications

ICC/IF

Species

Human

Katerina Artavanis-Tsakonas,Pia V Kasperkovitz,Eliseo Papa,Michael L Cardenas,Nida S Khan,Annemarthe G Van der Veen,Hidde L Ploegh,Jatin M Vyas

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD82 antibody [B-L2] - BSA and Azide free

Flow Cytometry - Anti-CD82 antibody [B-L2] - BSA and Azide free (AB47153)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD82 antibody [B-L2] - BSA and Azide free (AB47153)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise