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Mouse Monoclonal CD82 antibody. Suitable for Flow Cyt, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Cell preparation containing CD82 protein.

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Images

Flow Cytometry - Anti-CD82 antibody [C33] (AB140238), expandable thumbnail
  • Flow Cytometry - Anti-CD82 antibody [C33] (AB140238), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD82 antibody [C33] (AB140238), expandable thumbnail

Publications

Key facts

Isotype
IgG2a
Host species
Mouse
Storage buffer

pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: 99% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Cell preparation containing CD82 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P27701

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytIHC-P
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1.00000-4.00000 µg/mL
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

Structural component of specialized membrane microdomains known as tetraspanin-enriched microdomains (TERMs), which act as platforms for receptor clustering and signaling (PubMed:19497983). Participates thereby in diverse biological functions such as cell signal transduction, adhesion, migration and protein trafficking. Acts as a attenuator of EGF signaling, facilitating ligand-induced endocytosis of the receptor and its subsequent desensitization (PubMed:10985391, PubMed:35538033). Mechanistically, modulates ligand-induced ubiquitination and trafficking of EGFR via E3 ligase CBL phosphorylation by PKC (PubMed:23897813). Increases cell-matrix adhesion by regulating the membrane organization of integrin alpha4/ITA4 (PubMed:24623721, PubMed:8757325). Modulates adhesion and suppresses cell migration through other integrins such as the alpha6/ITGA6 and beta1/ITGB1 (PubMed:15557282, PubMed:17560548). Decreases cell-associated plasminogen activation by interfering with the interaction between urokinase-type plasminogen activator/PLAU and its receptor PLAUR (PubMed:15677461). Associates with CD4 or CD8 and delivers costimulatory signals for the TCR/CD3 pathway. Plays a role in TLR9 trafficking to acidified CpG-containing compartments by controlling interaction between TLR9 and VAMP3 and subsequent myddosome assembly (By similarity). Inhibits LPS-induced inflammatory response by preventing binding of LPS to TLR4 on the cell surface (PubMed:36945827). Plays a role in the activation of macrophages into anti-inflammatory phenotypes (By similarity). Independently of Toll-like receptor (TLR) signaling, is recruited to pathogen-containing phagosomes prior to fusion with lysosomes and thereby participates in antigen presentation (By similarity). Acts also to control angiogenesis and switch angiogenic milieu to quiescent state by binding and sequestering VEGFA and PDGFB to inhibit the signaling they trigger via their respective cell surface receptor (PubMed:34530889).

Alternative names

CD82, KAI1, SAR2, ST6, TSPAN27, CD82 antigen, C33 antigen, IA4, Inducible membrane protein R2, Metastasis suppressor Kangai-1, Suppressor of tumorigenicity 6 protein, Tetraspanin-27, Tspan-27

Recommended products

Mouse Monoclonal CD82 antibody. Suitable for Flow Cyt, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Cell preparation containing CD82 protein.

Key facts

Isotype
IgG2a
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Cell preparation containing CD82 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P27701
Clone number
C33
Purification technique
Affinity purification Protein A
Concentration
Loading...
Purification notes

ab140238 was purified from cell culture supernatant by protein A affinity chromatography to obtain purity of > 95% (by SDS-PAGE).

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle, Store undiluted

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CD82 also known as KAI1 is a member of the tetraspanin family of proteins. It has a molecular mass of approximately 29 to 30 kDa. This protein is widely expressed on the surface of various cell types such as epithelial cells lymphocytes and endothelial cells. It features four transmembrane domains implying a role in organizing membrane microdomains. CD82 is known for its role in promoting cell adhesion and motility through its involvement in protein-protein interactions on the cell surface.

Biological function summary

CD82 plays a significant role in regulating cell signaling and communication during tumor progression and metastasis. It participates in the formation of tetraspanin-enriched microdomains by associating with other tetraspanins and integrins influencing the cellular microenvironment. Additionally CD82 impacts immune cell interactions by modulating activities of T cells and B cells. Despite not being the predominant component in any major protein complex CD82 influences many cellular processes by regulating diverse signaling pathways.

Pathways

CD82 modulates both the PI3K-Akt signaling pathway and the Wnt signaling pathway. These pathways are critical in controlling cellular processes like growth and survival contributing to the metastasis-suppressive effects of CD82. In these contexts CD82 associates with proteins such as integrins and cadherins which facilitate its regulation of signal transduction and cellular dynamics. CD82's role in these pathways shows its importance in controlling cancer cell behavior and immune system interactions.

Associated diseases and disorders

CD82 has been linked to various cancers particularly prostate cancer and melanoma. Its downregulation or loss often correlates with tumor progression and metastasis indicating its function as a metastasis suppressor. In cancer CD82 interacts with proteins like epidermal growth factor receptor (EGFR) and other integrins affecting disease progression by altering cell adhesion migration and invasion. CD82’s expression level serves as a potential biomarker for cancer prognosis and treatment outcomes.

Product promise

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3 product images

  • Flow Cytometry - Anti-CD82 antibody [C33] (ab140238), expandable thumbnail

    Flow Cytometry - Anti-CD82 antibody [C33] (ab140238)

    Surface staining flow cytometric analysis of human peripheral blood stained using 1μg/ml anti-human CD82 purified antibody GAM APC

  • Flow Cytometry - Anti-CD82 antibody [C33] (ab140238), expandable thumbnail

    Flow Cytometry - Anti-CD82 antibody [C33] (ab140238)

    Flow cytometric analysis (surface staining) of human peripheral whole blood showing separation of CD82 negative lymphocytes (black dashed), and CD82 positive lymphocytes (red filled). The concentration of Anti-human CD82 (C33) purified antibody in the sample was 1μg/ml.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD82 antibody [C33] (ab140238), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD82 antibody [C33] (ab140238)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling CD82 with ab140238.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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