AB109529

Anti-CD82 antibody [EPR4112]

RabMAb
Recombinant
What is this?

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(4 Publications)

Rabbit Recombinant Monoclonal CD82 antibody. Suitable for WB and reacts with Human samples. Cited in 4 publications.

View Alternative Names

CD82, KAI1, SAR2, ST6, TSPAN27, CD82 antigen, C33 antigen, IA4, Inducible membrane protein R2, Metastasis suppressor Kangai-1, Suppressor of tumorigenicity 6 protein, Tetraspanin-27, Tspan-27

2 Images

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Western blot - Anti-CD82 antibody [EPR4112] (AB109529)

Lanes 1 - 5:

Western blot - Anti-CD82 antibody [EPR4112] (ab109529) at 1/1000 dilution

Lanes 6 - 7:

Western blot - Anti-CD82 antibody [EPR4112] - BSA and Azide free (<a href='/en-us/products/primary-antibodies/cd82-antibody-epr4112-bsa-and-azide-free-ab226072'>ab226072</a>) at 1/1000 dilution

Lane 1:

U-87 MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysate

Lane 2:

Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate

Lane 3:

Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate

Lane 4:

SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate

Lane 5:

HT-29 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate

Lane 6:

RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate

Lane 7:

C6 (Rat glial tumor glial cell) whole cell lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 29 kDa

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CiteAb

Western blot - Anti-CD82 antibody [EPR4112] (AB109529)

CD82 western blot using anti-CD82 antibody [EPR4112] ab109529. Publication image and figure legend from Ding, C., Cheng, S., et al., 2014, Int J Mol Sci, PubMed 24663081.

ab109529 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab109529 please see the product overview.

Upregulation of QKI, CD82, and RBM38 were validated after knockdown of HOTAIR. (A) Bel-7402 cells; (B) HepG2 cells were transiently transfected with HOTAIR siRNA for 48 h to detect QKI, CD82, and RBM38 expression by qRT-PCR compared with the siGFP group (* indicated p < 0.05); and (C) showed the protein level of QKI, CD82, and RBM38 after HOTAIR knockdown in Bel-7402 cells by Western blot. All experiments were performed in triplicates.

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Carrier free

Anti-CD82 antibody [EPR4112] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4112

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }

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Product details

Species reactivity
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD82 also known as KAI1 is a member of the tetraspanin family of proteins. It has a molecular mass of approximately 29 to 30 kDa. This protein is widely expressed on the surface of various cell types such as epithelial cells lymphocytes and endothelial cells. It features four transmembrane domains implying a role in organizing membrane microdomains. CD82 is known for its role in promoting cell adhesion and motility through its involvement in protein-protein interactions on the cell surface.

Biological function summary

CD82 plays a significant role in regulating cell signaling and communication during tumor progression and metastasis. It participates in the formation of tetraspanin-enriched microdomains by associating with other tetraspanins and integrins influencing the cellular microenvironment. Additionally CD82 impacts immune cell interactions by modulating activities of T cells and B cells. Despite not being the predominant component in any major protein complex CD82 influences many cellular processes by regulating diverse signaling pathways.

Pathways

CD82 modulates both the PI3K-Akt signaling pathway and the Wnt signaling pathway. These pathways are critical in controlling cellular processes like growth and survival contributing to the metastasis-suppressive effects of CD82. In these contexts CD82 associates with proteins such as integrins and cadherins which facilitate its regulation of signal transduction and cellular dynamics. CD82's role in these pathways shows its importance in controlling cancer cell behavior and immune system interactions.

CD82 has been linked to various cancers particularly prostate cancer and melanoma. Its downregulation or loss often correlates with tumor progression and metastasis indicating its function as a metastasis suppressor. In cancer CD82 interacts with proteins like epidermal growth factor receptor (EGFR) and other integrins affecting disease progression by altering cell adhesion migration and invasion. CD82’s expression level serves as a potential biomarker for cancer prognosis and treatment outcomes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Structural component of specialized membrane microdomains known as tetraspanin-enriched microdomains (TERMs), which act as platforms for receptor clustering and signaling (PubMed : 19497983). Participates thereby in diverse biological functions such as cell signal transduction, adhesion, migration and protein trafficking. Acts as a attenuator of EGF signaling, facilitating ligand-induced endocytosis of the receptor and its subsequent desensitization (PubMed : 10985391, PubMed : 35538033). Mechanistically, modulates ligand-induced ubiquitination and trafficking of EGFR via E3 ligase CBL phosphorylation by PKC (PubMed : 23897813). Increases cell-matrix adhesion by regulating the membrane organization of integrin alpha4/ITA4 (PubMed : 24623721, PubMed : 8757325). Modulates adhesion and suppresses cell migration through other integrins such as the alpha6/ITGA6 and beta1/ITGB1 (PubMed : 15557282, PubMed : 17560548). Decreases cell-associated plasminogen activation by interfering with the interaction between urokinase-type plasminogen activator/PLAU and its receptor PLAUR (PubMed : 15677461). Associates with CD4 or CD8 and delivers costimulatory signals for the TCR/CD3 pathway. Plays a role in TLR9 trafficking to acidified CpG-containing compartments by controlling interaction between TLR9 and VAMP3 and subsequent myddosome assembly (By similarity). Inhibits LPS-induced inflammatory response by preventing binding of LPS to TLR4 on the cell surface (PubMed : 36945827). Plays a role in the activation of macrophages into anti-inflammatory phenotypes (By similarity). Independently of Toll-like receptor (TLR) signaling, is recruited to pathogen-containing phagosomes prior to fusion with lysosomes and thereby participates in antigen presentation (By similarity). Also acts to control angiogenesis and switch angiogenic milieu to quiescent state by binding and sequestering VEGFA and PDGFB to inhibit the signaling they trigger via their respective cell surface receptor (PubMed : 34530889).

See full target information CD82

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Glia 72:692-707 PubMed38192185

2024

Schwann cell-derived exosomes promote lung cancer progression via miRNA-21-5p.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Zhou,Yao Zhang,Jianlin Xu,Yue Wang,Yu Yang,Weimin Wang,Aiqin Gu,Baohui Han,Galina V Shurin,Runbo Zhong,Michael R Shurin,Hua Zhong

Technology in cancer research & treatment 20:15330338211045204 PubMed34605330

2021

Metabolomic Detection Between Pancreatic Cancer and Liver Metastasis Nude Mouse Models Constructed by Using the PANC1-KAI1/CD Cell Line.

Applications

Unspecified application

Species

Unspecified reactive species

Shuo Wang,Jiang Chen,Hongyu Li,Xingshun Qi,Xu Liu,Xiaozhong Guo

Disease markers 2020:8899924 PubMed33204367

2020

CD82 Suppresses ADAM17-Dependent E-Cadherin Cleavage and Cell Migration in Prostate Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenkun Ma,Ye Gao,Wei Liu,Long Zheng,Ben Jin,Bei Duan,Hongjun Xie,Peng Guo,Jin Zeng,Ke Wang,Shan Xu,Xinyang Wang,Dalin He,Lei Li

International journal of molecular sciences 15:4060-76 PubMed24663081

2014

Long non-coding RNA HOTAIR promotes cell migration and invasion via down-regulation of RNA binding motif protein 38 in hepatocellular carcinoma cells.

Applications

Species

Human

Chaofeng Ding,Shaobing Cheng,Zhe Yang,Zhen Lv,Heng Xiao,Chengli Du,Chuanhui Peng,Haiyang Xie,Lin Zhou,Jian Wu,Shusen Zheng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD82 antibody [EPR4112]

Western blot - Anti-CD82 antibody [EPR4112] (AB109529)

Western blot - Anti-CD82 antibody [EPR4112] (AB109529)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise