AB59509

Anti-CD82 antibody [TS82b] - BSA and Azide free

Name: Anti-CD82 antibody [TS82b] - BSA and Azide free (ab59509) | Abcam
Brand: Abcam Limited
SKU: AB59509
Rating: 5 (5 reviews)

(5 Reviews)

(12 Publications)

Mouse Monoclonal CD82 antibody. Carrier free. Suitable for Flow Cyt, WB and reacts with Human samples. Cited in 12 publications. Immunogen corresponding to Cell preparation containing CD82 protein.

View Alternative Names

CD82, KAI1, SAR2, ST6, TSPAN27, CD82 antigen, C33 antigen, IA4, Inducible membrane protein R2, Metastasis suppressor Kangai-1, Suppressor of tumorigenicity 6 protein, Tetraspanin-27, Tspan-27

2 Images

Flow Cytometry - Anti-CD82 antibody [TS82b] - BSA and Azide free (AB59509)

Flow Cyt

Unknown

Flow Cytometry - Anti-CD82 antibody [TS82b] - BSA and Azide free (AB59509)

Overlay histogram showing HeLa cells stained with ab59509 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59509, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was a goat anti-mouse DyLight^® 488 (IgG, H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.

Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

This image was generated using the ascites version of the product.

Supplier Data

Western blot - Anti-CD82 antibody [TS82b] - BSA and Azide free (AB59509)

Lysis buffer :
• Tris pH7.4, NaCl 150 mM, CaCl2 2H2O 1 mM, MgCl2 1 mM NaN3 0.02%, Triton X100 1%

Gel :
5-15% SDS polyacrylamide.

All lanes:

Western blot - Anti-CD82 antibody [TS82b] - BSA and Azide free (ab59509) at 1 µg/mL

Lane 1:

Raji (Human Burkitt's lymphoma cell line) whole cell lysate at 25 µg

Lane 2:

Raji whole cell lysate at 50 µg

Lane 3:

HEL (Human bone marrow erythroleukemia cell line) whole cell lysate at 25 µg

Lane 4:

HEL whole cell lysate at 50 µg

Lane 5:

YT whole cell lysate at 25 µg

Lane 6:

YT whole cell lysate at 50 µg

Secondary

All lanes:

Anti IgG1-HRP

Predicted band size: 29 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

TS82b

Isotype

IgG1

Light chain type

kappa

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt, WB

applications

Immunogen

Cell preparation containing CD82 protein. The exact immunogen used to generate this antibody is proprietary information.

P27701

Specificity

Recognises the CD82 antigen, a 60 kDa protein.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

This product was changed from ascites to tissue culture supernatant on 19^th June 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.3 - 7.5 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

Up to 12 months

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD82 also known as KAI1 is a member of the tetraspanin family of proteins. It has a molecular mass of approximately 29 to 30 kDa. This protein is widely expressed on the surface of various cell types such as epithelial cells lymphocytes and endothelial cells. It features four transmembrane domains implying a role in organizing membrane microdomains. CD82 is known for its role in promoting cell adhesion and motility through its involvement in protein-protein interactions on the cell surface.

Biological function summary

CD82 plays a significant role in regulating cell signaling and communication during tumor progression and metastasis. It participates in the formation of tetraspanin-enriched microdomains by associating with other tetraspanins and integrins influencing the cellular microenvironment. Additionally CD82 impacts immune cell interactions by modulating activities of T cells and B cells. Despite not being the predominant component in any major protein complex CD82 influences many cellular processes by regulating diverse signaling pathways.

Pathways

CD82 modulates both the PI3K-Akt signaling pathway and the Wnt signaling pathway. These pathways are critical in controlling cellular processes like growth and survival contributing to the metastasis-suppressive effects of CD82. In these contexts CD82 associates with proteins such as integrins and cadherins which facilitate its regulation of signal transduction and cellular dynamics. CD82's role in these pathways shows its importance in controlling cancer cell behavior and immune system interactions.

CD82 has been linked to various cancers particularly prostate cancer and melanoma. Its downregulation or loss often correlates with tumor progression and metastasis indicating its function as a metastasis suppressor. In cancer CD82 interacts with proteins like epidermal growth factor receptor (EGFR) and other integrins affecting disease progression by altering cell adhesion migration and invasion. CD82’s expression level serves as a potential biomarker for cancer prognosis and treatment outcomes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Structural component of specialized membrane microdomains known as tetraspanin-enriched microdomains (TERMs), which act as platforms for receptor clustering and signaling (PubMed : 19497983). Participates thereby in diverse biological functions such as cell signal transduction, adhesion, migration and protein trafficking. Acts as a attenuator of EGF signaling, facilitating ligand-induced endocytosis of the receptor and its subsequent desensitization (PubMed : 10985391, PubMed : 35538033). Mechanistically, modulates ligand-induced ubiquitination and trafficking of EGFR via E3 ligase CBL phosphorylation by PKC (PubMed : 23897813). Increases cell-matrix adhesion by regulating the membrane organization of integrin alpha4/ITA4 (PubMed : 24623721, PubMed : 8757325). Modulates adhesion and suppresses cell migration through other integrins such as the alpha6/ITGA6 and beta1/ITGB1 (PubMed : 15557282, PubMed : 17560548). Decreases cell-associated plasminogen activation by interfering with the interaction between urokinase-type plasminogen activator/PLAU and its receptor PLAUR (PubMed : 15677461). Associates with CD4 or CD8 and delivers costimulatory signals for the TCR/CD3 pathway. Plays a role in TLR9 trafficking to acidified CpG-containing compartments by controlling interaction between TLR9 and VAMP3 and subsequent myddosome assembly (By similarity). Inhibits LPS-induced inflammatory response by preventing binding of LPS to TLR4 on the cell surface (PubMed : 36945827). Plays a role in the activation of macrophages into anti-inflammatory phenotypes (By similarity). Independently of Toll-like receptor (TLR) signaling, is recruited to pathogen-containing phagosomes prior to fusion with lysosomes and thereby participates in antigen presentation (By similarity). Also acts to control angiogenesis and switch angiogenic milieu to quiescent state by binding and sequestering VEGFA and PDGFB to inhibit the signaling they trigger via their respective cell surface receptor (PubMed : 34530889).

See full target information CD82

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Journal of extracellular vesicles 12:e12358 PubMed37563857

2023

Comprehensive characterization of human brain-derived extracellular vesicles using multiple isolation methods: Implications for diagnostic and therapeutic applications.

Applications

Unspecified application

Species

Unspecified reactive species

Zhengrong Zhang,Kaiwen Yu,Yang You,Peizhou Jiang,Zhiping Wu,Michael A DeTure,Dennis W Dickson,Seiko Ikezu,Junmin Peng,Tsuneya Ikezu

Acta biochimica et biophysica Sinica 54:400-408 PubMed35538033

2022

CD82 palmitoylation site mutations at Cys5+Cys74 affect EGFR internalization and metabolism through recycling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jingya Bu,Weiliang Zhong,Meixian Li,Shuiqing He,Mingzhe Zhang,Yu Zhang,Ying Li

International journal of molecular sciences 23: PubMed35328683

2022

Extracellular Vesicles as Signal Carriers in Malignant Thyroid Tumors?

Applications

Unspecified application

Species

Unspecified reactive species

Małgorzata Grzanka,Anna Stachurska-Skrodzka,Anna Adamiok-Ostrowska,Ewa Gajda,Barbara Czarnocka

Gut : PubMed35012996

2022

Extracellular Vesicles from Pancreatic Cancer Stem Cells Lead an Intratumor Communication Network (EVNet) to fuel tumour progression.

Applications

Unspecified application

Species

Unspecified reactive species

Carolina F Ruivo,Nuno Bastos,Barbara Adem,Ines Batista,Cecilia Duraes,Carlos A Melo,Stephanie A Castaldo,Francisco Campos-Laborie,Pedro Moutinho-Ribeiro,Barbara Morão,Ana Costa-Pinto,Soraia Silva,Hugo Osorio,Sergio Ciordia,Jose Luis Costa,David Goodrich,Bruno Cavadas,Luisa Pereira,Tony Kouzarides,Guilherme Macedo,Rui Maio,Fatima Carneiro,Marília Cravo,Raghu Kalluri,Jose Carlos Machado,Sonia A Melo

Molecular medicine reports 22:3994-4002 PubMed33000220

2020

Gangliosides and CD82 inhibit the motility of colon cancer by downregulating the phosphorylation of EGFR at different tyrosine sites and signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaohua Huang,Ying Li,Xin He,Yang Chen,Wei Wei,Xuesong Yang,Keli Ma

GeroScience 42:595-611 PubMed32088828

2020

CD82-TRPM7-Numb signaling mediates age-related cognitive impairment.

Applications

Unspecified application

Species

Unspecified reactive species

Yin Zhao,Tamas Kiss,Jordan DelFavero,Lu Li,Xing Li,Lu Zheng,Jie Wang,Chao Jiang,Jing Shi,Zoltan Ungvari,Anna Csiszar,Xin A Zhang

International journal of clinical and experimental pathology 12:4361-4379 PubMed31933840

2019

miR-601 inhibits proliferation, migration and invasion of prostate cancer stem cells by targeting KRT5 to inactivate the Wnt signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hongbing Du,Xinghuan Wang,Rui Dong,Dongliang Hu,Yaoyi Xiong

Scientific reports 8:8822 PubMed29891991

2018

Extracellular vesicles with altered tetraspanin CD9 and CD151 levels confer increased prostate cell motility and invasion.

Applications

Unspecified application

Species

Unspecified reactive species

Joshua S Brzozowski,Danielle R Bond,Helen Jankowski,Belinda J Goldie,Rachel Burchell,Crystal Naudin,Nathan D Smith,Christopher J Scarlett,Martin R Larsen,Matthew D Dun,Kathryn A Skelding,Judith Weidenhofer

Histopathology 67:730-9 PubMed25828894

2015

Distinct expression patterns of Toll-like receptor 7 in tumour cells and fibroblast-like cells in oral squamous cell carcinoma.

Applications

IHC

Species

Unspecified reactive species

Yan Hong Ni,Liang Ding,Dong Ya Zhang,Ya Yi Hou,Xiaofeng Huang,Qingang Hu

Journal of immunology (Baltimore, Md. : 1950) 191:2796-805 PubMed23872050

2013

Identification of the tetraspanin CD82 as a new barrier to xenotransplantation.

Applications

Unspecified application

Species

Unspecified reactive species

Soad M Saleh,Ranjit S Parhar,Reem S Al-Hejailan,Razan H Bakheet,Hala S Khaleel,Hanif G Khalak,Anason S Halees,Marya Z Zaidi,Brian F Meyer,Gisella P Yung,Jörg D Seebach,Walter Conca,Khalid S Khabar,Kate S Collison,Futwan A Al-Mohanna

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD82 antibody [TS82b] - BSA and Azide free

Flow Cytometry - Anti-CD82 antibody [TS82b] - BSA and Azide free (AB59509)

Western blot - Anti-CD82 antibody [TS82b] - BSA and Azide free (AB59509)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Specificity

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

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Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (12)

Product promise