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Rabbit Recombinant Monoclonal CD83 antibody. Carrier free. Suitable for Flow Cyt, WB, ICC/IF and reacts with Human samples.

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Images

Flow Cytometry - Anti-CD83 antibody [EPR22405] - BSA and Azide free (AB245986), expandable thumbnail
  • Flow Cytometry - Anti-CD83 antibody [EPR22405] - BSA and Azide free (AB245986), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CD83 antibody [EPR22405] - BSA and Azide free (AB245986), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytWBIHC-PICC/IF
Human
Tested
Expected
Not recommended
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

6 products for Alternative Product

Target data

Function

May play a significant role in antigen presentation or the cellular interactions that follow lymphocyte activation.

Alternative names

CD83, CD83 antigen, hCD83, B-cell activation protein, Cell surface protein HB15

Recommended products

Rabbit Recombinant Monoclonal CD83 antibody. Carrier free. Suitable for Flow Cyt, WB, ICC/IF and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR22405
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab245986 is the carrier-free version of Anti-CD83 antibody [EPR22405] ab244204.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CD83 also known as 3D11 in some contexts is a protein with important roles in the immune system. The CD83 protein is a glycoprotein with a molecular mass of approximately 45 kDa. It is expressed in various immune cells including dendritic cells B cells thymic epithelial cells and activated T cells. CD83 acts as a surface marker during the maturation and activation of these cells. This characteristic makes it a useful marker in immunological studies often referred to by scientists as the CD83 marker.

Biological function summary

The primary role of CD83 involves the regulation of immune responses. It functions by modulating the activity and communication of immune cells especially impacting the antigen-presenting capabilities of dendritic cells. CD83 also plays a role in promoting the maturation of these cells and influencing T-cell responses. Although CD83 is not generally recognized as a part of a complex its interactions with other cell surface molecules are significant for immune system function.

Pathways

CD83 has notable involvement in immune signaling pathways including the NF-kB and JAK/STAT pathways. These pathways are critical for transmitting signals that influence cell survival proliferation and activation. Through these pathways CD83 interacts with proteins such as cytokines and growth factors which further modulate their effects on immune cells and their responses.

Associated diseases and disorders

Understanding CD83’s role provides insights into conditions like autoimmune diseases and immune deficiencies. In autoimmune diseases irregular expression or function of CD83 can disrupt normal immune response contributing to pathogenesis. Its relationship with other proteins such as CD28 in T-cell activation underlies its involvement in disorders characterized by immune dysregulation. Furthermore CD83 expression profiles may help decipher cancer immune evasion mechanisms providing possible therapeutic targets.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Flow Cytometry - Anti-CD83 antibody [EPR22405] - BSA and Azide free (ab245986), expandable thumbnail

    Flow Cytometry - Anti-CD83 antibody [EPR22405] - BSA and Azide free (ab245986)

    Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) labeling CD83 with Anti-CD83 antibody [EPR22405] ab244204 at 1/50 (Right panel) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Left panel). Goat anti rabbit IgG (Dylight® 488, ab98462), at 1/2000 dilution was used as the secondary antibody.

    Cells were stained with rabbit IgG (Left panel) or anti-CD83 RabMab (Right panel). Then stained with Alexa Fluor® 647-conjugated anti-CD14. The result is consistent with what has been described in the literature. (PMID: 15320871).

    Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD83 antibody [EPR22405] ab244204).

  • Flow Cytometry - Anti-CD83 antibody [EPR22405] - BSA and Azide free (ab245986), expandable thumbnail

    Flow Cytometry - Anti-CD83 antibody [EPR22405] - BSA and Azide free (ab245986)

    Flow cytometric analysis of HDLM-2 (human Hodgkin lymphoma) cell line labeling CD83 with Anti-CD83 antibody [EPR22405] ab244204 at 1/50 (red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), at 1/2000 dilution was used as the secondary antibody.

    Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD83 antibody [EPR22405] ab244204).

  • Immunocytochemistry/ Immunofluorescence - Anti-CD83 antibody [EPR22405] - BSA and Azide free (ab245986), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CD83 antibody [EPR22405] - BSA and Azide free (ab245986)

    Immunofluorescent analysis of HDLM-2 (human Hodgkin lymphoma) cells fixed with 100% methanol, labeling CD83 with Anti-CD83 antibody [EPR22405] ab244204 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous and cytoplasmic staining in HDLM-2 cells. We recommend using methanol instead of PFA fixation. The nuclear counter stain is DAPI (blue).
    Counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at a 1/200 dilution (red).
    The negative control is the secondary antibody only.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD83 antibody [EPR22405] ab244204).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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