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AB123494

Anti-CD83 antibody [HB15e]

0

(1 Review)

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(1 Publication)

Mouse Recombinant Monoclonal CD83 antibody. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication.

View Alternative Names

CD83, CD83 antigen, hCD83, B-cell activation protein, Cell surface protein HB15

2 Images
Flow Cytometry - Anti-CD83 antibody [HB15e] (AB123494)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD83 antibody [HB15e] (AB123494)

Flow Cytometry analysis of human peripheral blood mononuclear cell (PBMC) treated with 100ng/ml GM-CSF and 50ng/ml IL-4 for 3 days then change new medium (100ng/ml GM-CSF and 50ng/ml IL-4) for another 3 days (right panel) compared to mouse monoclonal IgG isotype control (left panel). ab123494 used at a 1/400 dilution. Secondary is Goat Anti-Mouse IgG (Alexa Fluor® 647, ab150119) used at a 1/2000 dilution. Gated on viable cells. Bright secondary antibody like Alexa Fluor®647 conjugated ones are recommended to get desirable signal.

Flow Cytometry - Anti-CD83 antibody [HB15e] (AB123494)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD83 antibody [HB15e] (AB123494)

Flow Cytometry analysis of human monocyte-derived dendritic cells treated with 100ng/mL Lipopolysaccharide (LPS) for 24 hours (Right) / Untreated control (Left).

ab123494 used at a 1/1000 dilution.

Secondary is Goat anti-Mouse IgG (Alexa Fluor® 488, ab150113) used at a 1/2000 dilution.

The cells were simultaneously stained with CD209. Acquisition of >30000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.

Events were gated on viable single cells.

  • Carrier free

    Anti-CD83 antibody [HB15e] - BSA and Azide free

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

HB15e

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/400", "FlowCyt-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }
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Product details

Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by July 2018 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
ab123494 is >95% pure as determined by SDS-PAGE.
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD83 also known as 3D11 in some contexts is a protein with important roles in the immune system. The CD83 protein is a glycoprotein with a molecular mass of approximately 45 kDa. It is expressed in various immune cells including dendritic cells B cells thymic epithelial cells and activated T cells. CD83 acts as a surface marker during the maturation and activation of these cells. This characteristic makes it a useful marker in immunological studies often referred to by scientists as the CD83 marker.
Biological function summary

The primary role of CD83 involves the regulation of immune responses. It functions by modulating the activity and communication of immune cells especially impacting the antigen-presenting capabilities of dendritic cells. CD83 also plays a role in promoting the maturation of these cells and influencing T-cell responses. Although CD83 is not generally recognized as a part of a complex its interactions with other cell surface molecules are significant for immune system function.

Pathways

CD83 has notable involvement in immune signaling pathways including the NF-kB and JAK/STAT pathways. These pathways are critical for transmitting signals that influence cell survival proliferation and activation. Through these pathways CD83 interacts with proteins such as cytokines and growth factors which further modulate their effects on immune cells and their responses.

Understanding CD83's role provides insights into conditions like autoimmune diseases and immune deficiencies. In autoimmune diseases irregular expression or function of CD83 can disrupt normal immune response contributing to pathogenesis. Its relationship with other proteins such as CD28 in T-cell activation underlies its involvement in disorders characterized by immune dysregulation. Furthermore CD83 expression profiles may help decipher cancer immune evasion mechanisms providing possible therapeutic targets.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transmembrane glycoprotein predominantly found on the surface of many immune cells including dendritic cells or lymphocytes that plays various roles in immune response regulation. Plays an essential role in CD4(+) T-selection, differentiation and stability by regulating the activity of the major E3 ubiquitin ligase responsible for controlling MHCII trafficking MARCHF8. Also inhibits MARCHF1 association with MHCII or CD86 to prevent their ubiquitination and subsequent degradation (PubMed : 21220452). In addition, acts as an important modulator of protective responses against acute infections (By similarity).
See full target information CD83
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed37446144

2023

Selective Killing of BRCA2-Deficient Ovarian Cancer Cells via MRE11 Blockade.

Applications

Unspecified application

Species

Unspecified reactive species

Adel Alblihy,Reem Ali,Mashael Algethami,Alison A Ritchie,Ahmed Shoqafi,Shatha Alqahtani,Katia A Mesquita,Michael S Toss,Paloma Ordóñez-Morán,Jennie N Jeyapalan,Lodewijk Dekker,Martina Salerno,Edgar Hartsuiker,Anna M Grabowska,Emad A Rakha,Nigel P Mongan,Srinivasan Madhusudan
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com