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Rabbit Recombinant Monoclonal CD84 antibody. Carrier free. Suitable for Flow Cyt, WB and reacts with Human samples.

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Images

Flow Cytometry - Anti-CD84 antibody [EPR8324] - BSA and Azide free (AB226163), expandable thumbnail
  • Western blot - Anti-CD84 antibody [EPR8324] - BSA and Azide free (AB226163), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPFlow CytWBIHC-P
Human
Not recommended
Not recommended
Tested
Tested
Not recommended

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

5 products for Alternative Version

Target data

Function

Self-ligand receptor of the signaling lymphocytic activation molecule (SLAM) family. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response. Activities are controlled by presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2. Can mediate natural killer (NK) cell cytotoxicity dependent on SH2D1A and SH2D1B (By similarity). Increases proliferative responses of activated T-cells and SH2D1A/SAP does not seem be required for this process. Homophilic interactions enhance interferon gamma/IFNG secretion in lymphocytes and induce platelet stimulation via a SH2D1A-dependent pathway. May serve as a marker for hematopoietic progenitor cells (PubMed:11564780, PubMed:12115647, PubMed:12928397, PubMed:12962726, PubMed:16037392) Required for a prolonged T-cell:B-cell contact, optimal T follicular helper function, and germinal center formation. In germinal centers involved in maintaining B-cell tolerance and in preventing autoimmunity (By similarity). In mast cells negatively regulates high affinity immunoglobulin epsilon receptor signaling; independent of SH2D1A and SH2D1B but implicating FES and PTPN6/SHP-1 (PubMed:22068234). In macrophages enhances LPS-induced MAPK phosphorylation and NF-kappaB activation and modulates LPS-induced cytokine secretion; involving ITSM 2 (By similarity). Positively regulates macroautophagy in primary dendritic cells via stabilization of IRF8; inhibits TRIM21-mediated proteasomal degradation of IRF8 (PubMed:29434592).

Alternative names

CD84, SLAMF5, SLAM family member 5, Cell surface antigen MAX.3, Hly9-beta, Leukocyte differentiation antigen CD84, Signaling lymphocytic activation molecule 5

Recommended products

Rabbit Recombinant Monoclonal CD84 antibody. Carrier free. Suitable for Flow Cyt, WB and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR8324
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab226163 is the carrier-free version of Anti-CD84 antibody [EPR8324] ab137049.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CD84 also known as SLAMF5 is a cell surface glycoprotein with a molecular weight of approximately 64 kDa. It belongs to the signaling lymphocyte activation molecule (SLAM) family. CD84 is expressed on various immune cells including B cells T cells dendritic cells monocytes and platelets. This protein plays a role in cell signaling facilitating interactions between immune cells by acting as a homophilic receptor.

Biological function summary

CD84 contributes to the regulation of immune responses and intercellular communication. It is a part of the SLAM family which involves a signaling complex known as SLAM-associated protein (SAP) pathway. CD84 aids in the modulation of cell survival cytokine production and calcium mobilization thereby influencing immune cell function and maintenance.

Pathways

CD84 plays a role in key immune signaling pathways including the SAP signaling network and T-cell receptor pathways. CD84 interacts closely with SAP and other related proteins such as SLAM and 2B4 which help propagate T-cell activation signals and influence immune regulation. This position underlines CD84's engagement in regulating immune surveillance and homeostasis.

Associated diseases and disorders

CD84 has been implicated in autoimmune conditions and hematological malignancies. For instance it associates with systemic lupus erythematosus where altered expression may contribute to dysregulated immune responses. CD84 also plays a role in chronic lymphocytic leukemia correlating with disease progression and immune evasion. Its connection with proteins like SLAMF6 and SAP further highlights CD84's involvement in these pathological conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Flow Cytometry - Anti-CD84 antibody [EPR8324] - BSA and Azide free (ab226163), expandable thumbnail

    Flow Cytometry - Anti-CD84 antibody [EPR8324] - BSA and Azide free (ab226163)

    This data was developed using Anti-CD84 antibody [EPR8324] ab137049, the same antibody clone in a different buffer formulation.Flow cytometric analysis of Raji cells labelling CD84 with Anti-CD84 antibody [EPR8324] ab137049 at dilution 1/100 (red) or Rabbit IgG negative control (green).

  • Western blot - Anti-CD84 antibody [EPR8324] - BSA and Azide free (ab226163), expandable thumbnail

    Western blot - Anti-CD84 antibody [EPR8324] - BSA and Azide free (ab226163)

    This data was developed using Anti-CD84 antibody [EPR8324] ab137049, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-CD84 antibody [EPR8324] - BSA and Azide free (ab226163) at 1/1000 dilution

    Lane 1: Namalwa cell lysate at 10 µg

    Lane 2: Raji cell lysate at 10 µg

    Secondary

    All lanes: HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

    Predicted band size: 39 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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