Rabbit Recombinant Monoclonal CD84 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications.
Images
![Western blot - Anti-CD84 antibody [EPR8325] (AB131256), expandable thumbnail](https://content.abcam.com/products/images/cd84-antibody-epr8325-ab131256--western-blot-img124763.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CD84 antibody [EPR8325] (AB131256)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD84 antibody [EPR8325] (AB131256), expandable thumbnail](https://content.abcam.com/products/images/cd84-antibody-epr8325-ab131256--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img89902.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD84 antibody [EPR8325] (AB131256)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD84 antibody [EPR8325] (AB131256), expandable thumbnail](https://content.abcam.com/products/images/cd84-antibody-epr8325-ab131256--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img109656.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD84 antibody [EPR8325] (AB131256)")
Publications
Filter by
- Mediators of inflammation 2014:6317872014The histopathology of labial salivary glands in primary Sjögren's syndrome: focusing on follicular helper T cells in the inflammatory infiltrates.
- Clinical and experimental immunology 177:203-112014CD84 is markedly up-regulated in Kawasaki disease arteriopathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesR Reindel,J Bischof,K-Y A Kim,J M Orenstein,M B Soares,S C Baker,S T Shulman,E J Perlman,M W Lingen,A J Pink,C Trevenen,A H RowleyPubMed 24635044
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | Flow Cyt | WB | |
|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/25 - 1/50 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Self-ligand receptor of the signaling lymphocytic activation molecule (SLAM) family. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response. Activities are controlled by presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2. Can mediate natural killer (NK) cell cytotoxicity dependent on SH2D1A and SH2D1B (By similarity). Increases proliferative responses of activated T-cells and SH2D1A/SAP does not seem be required for this process. Homophilic interactions enhance interferon gamma/IFNG secretion in lymphocytes and induce platelet stimulation via a SH2D1A-dependent pathway. May serve as a marker for hematopoietic progenitor cells (PubMed:11564780, PubMed:12115647, PubMed:12928397, PubMed:12962726, PubMed:16037392) Required for a prolonged T-cell:B-cell contact, optimal T follicular helper function, and germinal center formation. In germinal centers involved in maintaining B-cell tolerance and in preventing autoimmunity (By similarity). In mast cells negatively regulates high affinity immunoglobulin epsilon receptor signaling; independent of SH2D1A and SH2D1B but implicating FES and PTPN6/SHP-1 (PubMed:22068234). In macrophages enhances LPS-induced MAPK phosphorylation and NF-kappaB activation and modulates LPS-induced cytokine secretion; involving ITSM 2 (By similarity). Positively regulates macroautophagy in primary dendritic cells via stabilization of IRF8; inhibits TRIM21-mediated proteasomal degradation of IRF8 (PubMed:29434592).
Alternative names
CD84, SLAMF5, SLAM family member 5, Cell surface antigen MAX.3, Hly9-beta, Leukocyte differentiation antigen CD84, Signaling lymphocytic activation molecule 5
Recommended products
Rabbit Recombinant Monoclonal CD84 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR8325
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD84 also known as SLAMF5 is a cell surface glycoprotein with a molecular weight of approximately 64 kDa. It belongs to the signaling lymphocyte activation molecule (SLAM) family. CD84 is expressed on various immune cells including B cells T cells dendritic cells monocytes and platelets. This protein plays a role in cell signaling facilitating interactions between immune cells by acting as a homophilic receptor.
- Biological function summary
CD84 contributes to the regulation of immune responses and intercellular communication. It is a part of the SLAM family which involves a signaling complex known as SLAM-associated protein (SAP) pathway. CD84 aids in the modulation of cell survival cytokine production and calcium mobilization thereby influencing immune cell function and maintenance.
- Pathways
CD84 plays a role in key immune signaling pathways including the SAP signaling network and T-cell receptor pathways. CD84 interacts closely with SAP and other related proteins such as SLAM and 2B4 which help propagate T-cell activation signals and influence immune regulation. This position underlines CD84's engagement in regulating immune surveillance and homeostasis.
- Associated diseases and disorders
CD84 has been implicated in autoimmune conditions and hematological malignancies. For instance it associates with systemic lupus erythematosus where altered expression may contribute to dysregulated immune responses. CD84 also plays a role in chronic lymphocytic leukemia correlating with disease progression and immune evasion. Its connection with proteins like SLAMF6 and SAP further highlights CD84's involvement in these pathological conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-CD84 antibody [EPR8325] (ab131256), expandable thumbnail](https://content.abcam.com/products/images/cd84-antibody-epr8325-ab131256--western-blot-img124763.jpg "Western blot - Anti-CD84 antibody [EPR8325] (ab131256)")
Western blot - Anti-CD84 antibody [EPR8325] (ab131256)
All lanes: Western blot - Anti-CD84 antibody [EPR8325] (ab131256) at 1/1000 dilution
Lane 1: Raji cell lysate at 10 µg
Lane 2: Namalwa cell lysate at 10 µg
SecondaryAll lanes: HRP labelled Goat anti Rabbit IgG at 1/2000 dilution
Predicted band size: 39 kDa
Observed band size: 64-82 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD84 antibody [EPR8325] (ab131256), expandable thumbnail](https://content.abcam.com/products/images/cd84-antibody-epr8325-ab131256--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img89902.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD84 antibody [EPR8325] (ab131256)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD84 antibody [EPR8325] (ab131256)
Immunohistochemical analysis of paraffin embedded Human spleen tissue labelling CD84 with ab131256 antibody at 1/25 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD84 antibody [EPR8325] (ab131256), expandable thumbnail](https://content.abcam.com/products/images/cd84-antibody-epr8325-ab131256--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img109656.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD84 antibody [EPR8325] (ab131256)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD84 antibody [EPR8325] (ab131256)
Immunohistochemical analysis of paraffin embedded Human tonsil tissue labelling CD84 with ab131256 antibody at 1/25 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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