Rabbit Recombinant Monoclonal CD86 antibody. Suitable for IHC-P, ICC, IP, WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human CD86 aa 300 to C-terminus.
Images
![Immunoprecipitation - Anti-CD86 antibody [BLR030F] - BSA free (AB243887), expandable thumbnail](https://content.abcam.com/products/images/cd86-antibody-blr030f-bsa-free-ab243887--immunoprecipitation-img92541.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-CD86 antibody [BLR030F] - BSA free (AB243887)")
![Immunocytochemistry - Anti-CD86 antibody [BLR030F] - BSA free (AB243887), expandable thumbnail](https://content.abcam.com/products/images/cd86-antibody-blr030f-bsa-free-ab243887--immunocytochemistry-img30349.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry - Anti-CD86 antibody [BLR030F] - BSA free (AB243887)")
![Western blot - Anti-CD86 antibody [BLR030F] - BSA free (AB243887), expandable thumbnail](https://content.abcam.com/products/images/cd86-antibody-blr030f-bsa-free-ab243887--western-blot-img118958.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CD86 antibody [BLR030F] - BSA free (AB243887)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD86 antibody [BLR030F] - BSA free (AB243887), expandable thumbnail](https://content.abcam.com/products/images/cd86-antibody-blr030f-bsa-free-ab243887--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img132333.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD86 antibody [BLR030F] - BSA free (AB243887)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD86 antibody [BLR030F] - BSA free (AB243887), expandable thumbnail](https://content.abcam.com/products/images/cd86-antibody-blr030f-bsa-free-ab243887--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img18081.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD86 antibody [BLR030F] - BSA free (AB243887)")
Publications
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- Frontiers in cell and developmental biology 9:6848422022LncRNA TCONS_00145741 Knockdown Prevents Thrombin-Induced M1 Differentiation of Microglia in Intracerebral Hemorrhage by Enhancing the Interaction Between DUSP6 and JNK.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLanxiang Wu,Qingqing Zhan,Pan Liu,Heqing Zheng,Mingxu Liu,Jun Min,Liang Xie,Wei WuPubMed 35127692
- Pharmaceutical biology 59:1139-11492021Bioadhesive hydrogel comprising bilirubin/β-cyclodextrin inclusion complexes promote diabetic wound healing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQing Yao,Yannan Shi,Xing Xia,Yingying Tang,Xue Jiang,Ya-Wen Zheng,Hailin Zhang,Ruijie Chen,Longfa KouPubMed 34425063
- Frontiers in oncology 11:6543502021Integrated Analysis Reveals Prognostic Value and Immune Correlates of CD86 Expression in Lower Grade Glioma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuaide Qiu,Wei Tian,Yikang He,Jiahui Li,Chuan He,Yongqiang Li,Ning Liu,Jianan LiPubMed 33954112
- Cell death & disease 12:3142021Oleandrin, a cardiac glycoside, induces immunogenic cell death via the PERK/elF2α/ATF4/CHOP pathway in breast cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoxi Li,Jian Zheng,Shi Chen,Fan-Dong Meng,Jing Ning,Shu-Lan SunPubMed 33762577
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 99% Borate buffered saline- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Synthetic Peptide within Human CD86 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P42081
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Reactivity data
Select an application| IHC-P | ICC | IP | WB | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Use 6μl/0.5mg lysate. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Associated Products
Select an associated product type
10 products for Alternative Product
Target data
Function
Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4 (PubMed:12196291). May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation (PubMed:7527824). Also involved in the regulation of B cells function, plays a role in regulating the level of IgG(1) produced. Upon CD40 engagement, activates NF-kappa-B signaling pathway via phospholipase C and protein kinase C activation (By similarity). Isoform 2. Interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation. (Microbial infection) Acts as a receptor for adenovirus subgroup B.
Alternative names
CD86, CD28LG2, T-lymphocyte activation antigen CD86, Activation B7-2 antigen, B70, BU63, CTLA-4 counter-receptor B7.2, FUN-1
Recommended products
Rabbit Recombinant Monoclonal CD86 antibody. Suitable for IHC-P, ICC, IP, WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human CD86 aa 300 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 99% Borate buffered saline- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Synthetic Peptide within Human CD86 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P42081
- Clone number
- BLR030F
- Concentration
- Purification notes
Recombinant antibody was purified from cell culture supernatant.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product is sold under License from Bethyl Laboratories, Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD86 also known as B7-2 is a protein involved in the regulation of the immune response. It has an approximate mass of 70 kDa and is expressed on antigen-presenting cells like dendritic cells monocytes and macrophages. Notably CD86 is present on macrophages including those in tissues such as skin and lymphoid organs. Expressed on these cells CD86 serves as a vital mediator in the co-stimulatory signals necessary for T cell activation and survival.
- Biological function summary
CD86 plays a significant role in the immune system by providing secondary signals for T cell activation and differentiation. It is a part of the B7 protein family and forms a complex with CD28 and CTLA-4 on T cells. When CD86 binds to CD28 it sends positive co-stimulatory signals which promote T cell proliferation and cytokine production. On the other hand interaction with CTLA-4 transmits an inhibitory signal which reduces immune response. This dual interaction helps to balance immune activation and tolerance.
- Pathways
CD86 takes part in important immune-related signaling pathways particularly the T cell receptor signaling pathway and the PI3K-Akt signaling pathway. Both pathways are fundamental for initiating immune responses. CD86's interaction with CD28 activates downstream signaling cascades including PI3K-Akt which is important for cell survival and growth. Additionally CD86 collaborates with other proteins such as CD80 another co-stimulatory molecule to amplify T cell activation within these pathways.
- Associated diseases and disorders
CD86 is associated with autoimmune diseases and transplant rejection. In autoimmune diseases like rheumatoid arthritis the overexpression or dysregulation of CD86 can lead to excessive T cell activation causing immune system attacks on the body's own tissues. Similarly in transplant rejection CD86 may contribute by enhancing immune response against transplanted organs. The engagement between CD86 and CD28 is a critical factor in these conditions and therapies targeting this interaction are under exploration to mitigate the immune response.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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5 product images
![Immunoprecipitation - Anti-CD86 antibody [BLR030F] - BSA free (ab243887), expandable thumbnail](https://content.abcam.com/products/images/cd86-antibody-blr030f-bsa-free-ab243887--immunoprecipitation-img92541.jpg "Immunoprecipitation - Anti-CD86 antibody [BLR030F] - BSA free (ab243887)")
Immunoprecipitation - Anti-CD86 antibody [BLR030F] - BSA free (ab243887)
CD86 was immunoprecipitated from 1.0 mg HDLM-2 (Human Hodgkin lymphoma cell line from pleural effusion) whole cell lysate with ab243887 at 6 μl per reaction. Western blot was performed on the immunoprecipitate using ab243887 at 1/1000 dilution.
Lane 1: Anti-PTPN12 antibody [BL-5-2F8] ab243864 IP in HDLM-2whole cell lysate.
Lane 2: Control IgG IP in HDLM-2 whole cell lysate.
Detection: Chemiluminescence with an exposure time of 10 seconds.
All lanes: Immunoprecipitation - Anti-CD86 antibody [BLR030F] - BSA free (ab243887)
Predicted band size: 38 kDa
![Immunocytochemistry - Anti-CD86 antibody [BLR030F] - BSA free (ab243887), expandable thumbnail](https://content.abcam.com/products/images/cd86-antibody-blr030f-bsa-free-ab243887--immunocytochemistry-img30349.jpg "Immunocytochemistry - Anti-CD86 antibody [BLR030F] - BSA free (ab243887)")
Immunocytochemistry - Anti-CD86 antibody [BLR030F] - BSA free (ab243887)
Formalin-fixed, paraffin-embedded HDLM-2 cells labeling CD86 using ab243887 at 1/200 dilution in ICC analysis. A HRP-conjugated goat-anti rabbit IgG was used as the secondary. DAB staining.
![Western blot - Anti-CD86 antibody [BLR030F] - BSA free (ab243887), expandable thumbnail](https://content.abcam.com/products/images/cd86-antibody-blr030f-bsa-free-ab243887--western-blot-img118958.jpg "Western blot - Anti-CD86 antibody [BLR030F] - BSA free (ab243887)")
Western blot - Anti-CD86 antibody [BLR030F] - BSA free (ab243887)
Detection: chemiluminescence with an exposure time of 3 minutes.
All lanes: Western blot - Anti-CD86 antibody [BLR030F] - BSA free (ab243887) at 1/1000 dilution
Lane 1: HDLM-2 (Human Hodgkin lymphoma cell line from pleural effusion) whole cell lysate at 5 µg
Lane 2: MJ (Human primary cutaneous T-cell non-Hodgkin lymphoma from peripheral blood) whole cell lysate at 5 µg
Lane 3: SUP-M2 (Human ALK-positive anaplastic large cell lymphoma from cerebrospinal fluid) whole cell lysate at 50 µg
Lane 4: KG-1 (Human acute myeloid leukemia from bone narrow) whole cell lysate at 50 µg
Lane 5: MOLT-4 (Human lymphoblastic leukemia cell line) whole cell lysate at 50 µg
Lane 6: Ramos (Human Burkitt's lymphoma cell line) whole cell lysate at 50 µg
Lane 7: Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Lane 8: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 50 µg
Lane 9: HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 10: RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 50 µg
SecondaryAll lanes: HRP-conjugated goat anti-rabbit IgG antibody
Predicted band size: 38 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD86 antibody [BLR030F] - BSA free (ab243887), expandable thumbnail](https://content.abcam.com/products/images/cd86-antibody-blr030f-bsa-free-ab243887--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img132333.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD86 antibody [BLR030F] - BSA free (ab243887)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD86 antibody [BLR030F] - BSA free (ab243887)
Formalin-fixed, paraffin-embedded human seminoma tissue stained for CD86 using Anti-HIF-1 alpha antibody [BL-124-3F7] ab243860 at 1/200 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD86 antibody [BLR030F] - BSA free (ab243887), expandable thumbnail](https://content.abcam.com/products/images/cd86-antibody-blr030f-bsa-free-ab243887--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img18081.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD86 antibody [BLR030F] - BSA free (ab243887)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD86 antibody [BLR030F] - BSA free (ab243887)
Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD86 using ab243887 at 1/200 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.
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