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AB234000

Anti-CD86 antibody [BU63]

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(8 Publications)

Mouse Monoclonal CD86 antibody. Suitable for Flow Cyt, IHC-P and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Cell preparation containing CD86 protein.

View Alternative Names

CD86, CD28LG2, T-lymphocyte activation antigen CD86, Activation B7-2 antigen, B70, BU63, CTLA-4 counter-receptor B7.2, FUN-1

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD86 antibody [BU63] (AB234000)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD86 antibody [BU63] (AB234000)

Formalin-fixed, paraffin-embedded human esophageal tumor tissue stained for CD86 using ab234000 at 4 μg/ml in immunohistochemical analysis.

Flow Cytometry - Anti-CD86 antibody [BU63] (AB234000)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD86 antibody [BU63] (AB234000)

Flow cytometric analysis of human PMBCs labeling CD86 with ab234000 at 1 μg/106 cells (red).

  • 667 PE/Cy5®

    PE/Cy5® Anti-CD86 antibody [BU63]

  • 675 PerCP

    PerCP Anti-CD86 antibody [BU63]

  • 519 FITC

    FITC Anti-CD86 antibody [BU63]

  • 778 PE/Cy7®

    PE/Cy7® Anti-CD86 antibody [BU63]

  • 660 APC

    APC Anti-CD86 antibody [BU63]

  • 578 PE

    PE Anti-CD86 antibody [BU63]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

BU63

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

IHC-P, Flow Cyt

applications

Immunogen

Cell preparation containing CD86 protein. The exact immunogen used to generate this antibody is proprietary information.

P42081

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.5-1 µg for 10^6 Cells", "FlowCyt-species-notes": "<p>(in 0.1 ml).</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2-4 µg/mL", "IHCP-species-notes": "<p>(Primary incubation for 30 minutes at RT).</p>" }, "Mouse": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Purification notes
Purified from Bioreactor Concentrate by Protein A/G.
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD86 also known as B7-2 is a protein involved in the regulation of the immune response. It has an approximate mass of 70 kDa and is expressed on antigen-presenting cells like dendritic cells monocytes and macrophages. Notably CD86 is present on macrophages including those in tissues such as skin and lymphoid organs. Expressed on these cells CD86 serves as a vital mediator in the co-stimulatory signals necessary for T cell activation and survival.
Biological function summary

CD86 plays a significant role in the immune system by providing secondary signals for T cell activation and differentiation. It is a part of the B7 protein family and forms a complex with CD28 and CTLA-4 on T cells. When CD86 binds to CD28 it sends positive co-stimulatory signals which promote T cell proliferation and cytokine production. On the other hand interaction with CTLA-4 transmits an inhibitory signal which reduces immune response. This dual interaction helps to balance immune activation and tolerance.

Pathways

CD86 takes part in important immune-related signaling pathways particularly the T cell receptor signaling pathway and the PI3K-Akt signaling pathway. Both pathways are fundamental for initiating immune responses. CD86's interaction with CD28 activates downstream signaling cascades including PI3K-Akt which is important for cell survival and growth. Additionally CD86 collaborates with other proteins such as CD80 another co-stimulatory molecule to amplify T cell activation within these pathways.

CD86 is associated with autoimmune diseases and transplant rejection. In autoimmune diseases like rheumatoid arthritis the overexpression or dysregulation of CD86 can lead to excessive T cell activation causing immune system attacks on the body's own tissues. Similarly in transplant rejection CD86 may contribute by enhancing immune response against transplanted organs. The engagement between CD86 and CD28 is a critical factor in these conditions and therapies targeting this interaction are under exploration to mitigate the immune response.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Costimulatory molecule that belongs to the immunoglobulin superfamily that plays an important role in T-lymphocyte activation (PubMed : 12196291, PubMed : 7694363). Acts as the primary auxiliary signal augmenting the MHC/TCR signal in naive T-cells by acting as a ligand for the CD28 receptor which is constitutively expressed on the cell surface of T-cells (PubMed : 12196291, PubMed : 7694363). May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation (PubMed : 7527824). Also involved in the regulation of B cells function, plays a role in regulating the level of IgG(1) produced. Upon CD40 engagement, activates NF-kappa-B signaling pathway via phospholipase C and protein kinase C activation (By similarity). Also acts as an inhibitor of T-cell activation by acting as a ligand for CTLA4, a decoy receptor, thereby blocking CD28-mediated T-cell priming (PubMed : 11279501).. Isoform 2. Interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.. (Microbial infection) Acts as a receptor for adenovirus subgroup B.
See full target information CD86
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Bioactive materials 50:364-381 PubMed40589547

2025

In situ implantation of type II collagen-based double-layer scaffolds for Articular Osteochondral Regeneration comprising hyaline cartilage and vascularized subchondral bones.

Applications

Unspecified application

Species

Unspecified reactive species

Zhen Zhang,Ye Huang,Xu Hu,Yulei Mu,Huiqun Zhou,Liang Ma,Bangheng Liu,Hang Yao,Xieyuan Jiang,Dong-An Wang

Journal of bone oncology 50:100655 PubMed39850453

2025

Using β-Elemene to reduce stemness and drug resistance in osteosarcoma: A focus on the AKT/FOXO1 signaling pathway and immune modulation.

Applications

Unspecified application

Species

Unspecified reactive species

Shaochun Zhang,Zhijie Xing,Jing Ke

The FEBS journal 291:5214-5235 PubMed39434428

2024

TBK1 is involved in M-CSF-induced macrophage polarization through mediating the IRF5/IRF4 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanyuan Li,Le Ji,Chang Liu,Juanjuan Li,Di Wen,Zhongyao Li,Lishuang Yu,Moran Guo,Shaoran Zhang,Weisong Duan,Le Yi,Yue Bi,Hui Bu,Chunyan Li,Yakun Liu

Cells 13: PubMed39329753

2024

Comparative Analysis of Inhibitory and Activating Immune Checkpoints PD-1, PD-L1, CD28, and CD86 in Non-Melanoma Skin Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Linus Winter,Jutta Ries,Christoph Vogl,Leah Trumet,Carol Immanuel Geppert,Rainer Lutz,Marco Kesting,Manuel Weber

Molecular therapy : the journal of the American Society of Gene Therapy 32:3990-4005 PubMed39295146

2024

Intranasal vaccination with engineered BCG expressing CCL2 induces a stronger immune barrier against Mycobacterium tuberculosis than BCG.

Applications

Unspecified application

Species

Unspecified reactive species

Shaohua Guo,Jiangshan Ouyang,Zhiming Hu,Ting Cao,Chunxu Huang,Jun Mou,Xinxia Gu,Jie Liu

Annals of translational medicine 10:976 PubMed36267713

2022

Exosomes derived from umbilical cord mesenchymal stem cells protect cartilage and regulate the polarization of macrophages in osteoarthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Pengdong Li,Shuang Lv,Wenyue Jiang,Lihui Si,Baojian Liao,Guifang Zhao,Ziran Xu,Lina Wang,Jia Zhang,Haitao Wu,Qian Peng,Zhaohui Li,Ling Qi,Guangfan Chi,Yulin Li

Oncology letters 23:9 PubMed34820008

2021

Role of Lamin A and emerin in maintaining nuclear morphology in different subtypes of ovarian epithelial cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Shiori Watabe,Sayaka Kobayashi,Mizuho Hatori,Yoshimi Nishijima,Naoki Inoue,Hayato Ikota,Akira Iwase,Hideaki Yokoo,Masanao Saio

Nature communications 12:4501 PubMed34301958

2021

Nitrate-functionalized patch confers cardioprotection and improves heart repair after myocardial infarction via local nitric oxide delivery.

Applications

Unspecified application

Species

Unspecified reactive species

Dashuai Zhu,Jingli Hou,Meng Qian,Dawei Jin,Tian Hao,Yanjun Pan,He Wang,Shuting Wu,Shuo Liu,Fei Wang,Lanping Wu,Yumin Zhong,Zhilu Yang,Yongzhe Che,Jie Shen,Deling Kong,Meng Yin,Qiang Zhao
View all publications
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Product promise

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