Anti-CD86 antibody [C86/1146]

• ICC

Supplier Data

Immunocytochemistry - Anti-CD86 antibody [C86/1146] (AB220188)

Immunofluorescence analysis of Raji (Human Burkitt's lymphoma cell line) cells labeling CD86 with ab220188 at 2 µg/mL followed by Goat anti-Mouse IgG-CF488 (green). The nuclear counterstain is Reddot (red).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD86 antibody [C86/1146] (AB220188)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labelling CD86 with ab220188 at 2 μg/mL for 30 minutes at room temperature. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at room temperature for 20 minutes.

• WB

Lab

Western blot - Anti-CD86 antibody [C86/1146] (AB220188)

Lanes 1 - 4 : Merged signal (red and green). Green - ab220188 observed at 70 kDa. Red - loading control ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) observed at 55 kDa.

ab220188 was shown to react with CD86 in wild-type Raji cells in Western blot with loss of signal observed in CD86 knockout cell line ab273812 (knockout cell lysate ab273858). Wild-type Raji and CD86 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween^®) before incubation with ab220188 and ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) overnight at 4 °C at 0.5 μg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye^® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye^® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-CD86 antibody [C86/1146] (ab220188) at 0.5 µg/mL

Lane 1:

Wild-type Raji cell lysate at 20 µg

Lane 2:

CD86 knockout Raji cell lysate at 20 µg

Lane 2:

Western blot - Human CD86 knockout Raji cell line (<a href='/en-us/products/cell-lines/human-cd86-knockout-raji-cell-line-ab273858'>ab273858</a>)

Lane 3:

Ramos cell lysate at 20 µg

Lane 4:

Human Liver tissue lysate at 20 µg

Predicted band size: 38 kDa

Observed band size: 70 kDa

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• WB

Supplier Data

Western blot - Anti-CD86 antibody [C86/1146] (AB220188)

All lanes:

Western blot - Anti-CD86 antibody [C86/1146] (ab220188) at 1 µg

All lanes:

Daudi cell lysate

Predicted band size: 38 kDa

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