Anti-CD86 antibody [C86/1146] is a mouse monoclonal antibody that is used to detect CD86 in ICC, IHC-P, Western blot. Suitable for Human samples.
Images
![Immunocytochemistry - Anti-CD86 antibody [C86/1146] (AB220188), expandable thumbnail](https://content.abcam.com/products/images/cd86-antibody-c86-1146-ab220188--immunocytochemistry-img13364.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry - Anti-CD86 antibody [C86/1146] (AB220188)")
![Western blot - Anti-CD86 antibody [C86/1146] (AB220188), expandable thumbnail](https://content.abcam.com/products/images/cd86-antibody-c86-1146-ab220188--western-blot-img170813.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CD86 antibody [C86/1146] (AB220188)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD86 antibody [C86/1146] (AB220188), expandable thumbnail](https://content.abcam.com/products/images/cd86-antibody-c86-1146-ab220188--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img18986.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD86 antibody [C86/1146] (AB220188)")
![Western blot - Anti-CD86 antibody [C86/1146] (AB220188), expandable thumbnail](https://content.abcam.com/products/images/cd86-antibody-c86-1146-ab220188--western-blot-img35456.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CD86 antibody [C86/1146] (AB220188)")
Publications
Filter by
- Stem cell research & therapy 14:3312023Extracellular vesicles derived from human ESC-MSCs target macrophage and promote anti-inflammation process, angiogenesis, and functional recovery in ACS-induced severe skeletal muscle injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiangkang Jiang,Jingyuan Yang,Yao Lin,Fei Liu,Jiawei Tao,Wenbin Zhang,Jiefeng Xu,Mao ZhangPubMed 37964317
- Advanced healthcare materials 12:e23019902023A Core-Shell Nanoreinforced Ion-Conductive Implantable Hydrogel Bioelectronic Patch with High Sensitivity and Bioactivity for Real-Time Synchronous Heart Monitoring and Repairing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSi Shen,Jie Zhang,Yanni Han,Chunyi Pu,Qixiang Duan,Jianxing Huang,Bing Yan,Xintong You,Rurong Lin,Xiaoxi Shen,Xiaozhong Qiu,Honghao HouPubMed 37467758
- Advanced healthcare materials 12:e22032012023Injectable Antibacterial Hydrogel with Asiaticoside-Loaded Liposomes and Ultrafine Silver Nanosilver Particles Promotes Healing of Burn-Infected Wounds.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLongbao Feng,Yu Liu,Yini Chen,Qi Xiang,Yadong Huang,Zonghua Liu,Wei Xue,Rui GuoPubMed 37195780
- Inflammation research : official journal of the European Histamine Research Society ... [et al.] 72:1237-12552023Rhein-attenuates LPS-induced acute lung injury via targeting NFATc1/Trem2 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiang Li,Chuan Xiao,Jia Yuan,Xianjun Chen,Qing Li,Feng ShenPubMed 37212865
- Aging 15:3715-37372023Plasmon-activated water as a therapeutic strategy in Alzheimer's disease by altering gut microbiota.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChia-Hsiung Cheng,Yu-Chuan Liu,Yu-Chen S H Yang,Kun-Ju Lin,Dean Wu,Yun-Ru Liu,Chun-Chao Chang,Chien-Tai Hong,Chaur-Jong HuPubMed 37166426
- Biomolecules 13:2023Identification of an Autophagy-Related Signature for Prognosis and Immunotherapy Response Prediction in Ovarian Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJinye Ding,Chunyan Wang,Yaoqi Sun,Jing Guo,Shupeng Liu,Zhongping ChengPubMed 36830707
- International journal of biological sciences 19:1123-11452023Low-intensity pulsed ultrasound promotes skeletal muscle regeneration via modulating the inflammatory immune microenvironment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaocheng Qin,Zhiwen Luo,Yaying Sun,Zhong He,Beijie Qi,Yisheng Chen,Junlong Wang,Ce Li,Weiwei Lin,Zhihua Han,Yulian ZhuPubMed 36923940
- Frontiers in bioengineering and biotechnology 10:10662662022Vascular endothelial growth factor attenuates neointimal hyperplasia of decellularized small-diameter vascular grafts by modulating the local inflammatory response.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXinlong Xie,Qiying Wu,Yuhong Liu,Chunyang Chen,Zeguo Chen,Chao Xie,Mingzhe Song,Zhenlin Jiang,Xiaoke Qi,Sixi Liu,Zhenjie Tang,Zhongshi WuPubMed 36605251
- Nature communications 13:76662022A smart adhesive Janus hydrogel for non-invasive cardiac repair and tissue adhesion prevention.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYutong He,Qian Li,Pinger Chen,Qixiang Duan,Jiamian Zhan,Xiaohui Cai,Leyu Wang,Honghao Hou,Xiaozhong QiuPubMed 36509756
- Frontiers in immunology 13:10013812022A specific immune signature for predicting the prognosis of glioma patients with IDH1-mutation and guiding immune checkpoint blockade therapy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhirui Zeng,Chujiao Hu,Wanyuan Ruan,Jinjuan Zhang,Shan Lei,Yushi Yang,Pailan Peng,Feng Pan,Tengxiang ChenPubMed 36159801
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human CD86. Database link P42081
Abcam Recommends
Reactivity data
Select an application| ICC | WB | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-2 µg/mL | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-2 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2-4 µg/mL | Notes For 30 minutes at RT. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
3 products for Alternative Product
Target data
Function
Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4 (PubMed:12196291). May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation (PubMed:7527824). Also involved in the regulation of B cells function, plays a role in regulating the level of IgG(1) produced. Upon CD40 engagement, activates NF-kappa-B signaling pathway via phospholipase C and protein kinase C activation (By similarity). Isoform 2. Interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation. (Microbial infection) Acts as a receptor for adenovirus subgroup B.
Alternative names
CD86, CD28LG2, T-lymphocyte activation antigen CD86, Activation B7-2 antigen, B70, BU63, CTLA-4 counter-receptor B7.2, FUN-1
Recommended products
Anti-CD86 antibody [C86/1146] is a mouse monoclonal antibody that is used to detect CD86 in ICC, IHC-P, Western blot. Suitable for Human samples.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human CD86. Database link P42081
- Clone number
- C86/1146
- Purification technique
- Affinity purification Protein A/G
- Light chain type
- kappa
- Concentration
- Purification notes
ab220188 is purified from Bioreactor Concentrate by Protein A/G column chromatography.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Product Specifications
Anti-CD86 antibody [C86/1146] (ab220188) is a mouse monoclonal antibody and is validated for use in ICC, IHC-P, WB in human samples.
Anti-CD86 antibody [C86/1146] (ab220188) specifically detects CD86 (UniProt ID: P42081; Molecular weight: 35kDa) and is sold in 100 µg selling sizes.
Quality and Validation
Abcam's high quality validation processes ensure Anti-CD86 antibody [C86/1146] (ab220188) has high sensitivity and specificity.
The specificity of Anti-CD86 antibody [C86/1146] (ab220188) has been confirmed by testing in knockout samples.
Anti-CD86 antibody [C86/1146] (ab220188) has been cited over 19 times in peer reviewed journals and is trusted by the scientific community.
Related Products
Conjugation-ready, carrier free format available for antibody clone C86/1146 - Anti-CD86 antibody [C86/1146] - BSA and Azide free ab213045.
Target Information
CD86 is a costimulatory molecule expressed on antigen-presenting cells, such as dendritic cells and macrophages. It plays a crucial role in T cell activation and survival by interacting with its receptors, CD28 and CTLA-4. CD86 is involved in various immune responses and is linked to diseases like autoimmune disorders and infections. CD68 is a key biomarker in autoimmune diseases such as Multiple Sclerosis (MS), Rheumatoid Arthritis (RA) and Systemic Lupus Erythematosus (SLE)
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD86 also known as B7-2 is a protein involved in the regulation of the immune response. It has an approximate mass of 70 kDa and is expressed on antigen-presenting cells like dendritic cells monocytes and macrophages. Notably CD86 is present on macrophages including those in tissues such as skin and lymphoid organs. Expressed on these cells CD86 serves as a vital mediator in the co-stimulatory signals necessary for T cell activation and survival.
- Biological function summary
CD86 plays a significant role in the immune system by providing secondary signals for T cell activation and differentiation. It is a part of the B7 protein family and forms a complex with CD28 and CTLA-4 on T cells. When CD86 binds to CD28 it sends positive co-stimulatory signals which promote T cell proliferation and cytokine production. On the other hand interaction with CTLA-4 transmits an inhibitory signal which reduces immune response. This dual interaction helps to balance immune activation and tolerance.
- Pathways
CD86 takes part in important immune-related signaling pathways particularly the T cell receptor signaling pathway and the PI3K-Akt signaling pathway. Both pathways are fundamental for initiating immune responses. CD86's interaction with CD28 activates downstream signaling cascades including PI3K-Akt which is important for cell survival and growth. Additionally CD86 collaborates with other proteins such as CD80 another co-stimulatory molecule to amplify T cell activation within these pathways.
- Associated diseases and disorders
CD86 is associated with autoimmune diseases and transplant rejection. In autoimmune diseases like rheumatoid arthritis the overexpression or dysregulation of CD86 can lead to excessive T cell activation causing immune system attacks on the body's own tissues. Similarly in transplant rejection CD86 may contribute by enhancing immune response against transplanted organs. The engagement between CD86 and CD28 is a critical factor in these conditions and therapies targeting this interaction are under exploration to mitigate the immune response.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunocytochemistry - Anti-CD86 antibody [C86/1146] (ab220188), expandable thumbnail](https://content.abcam.com/products/images/cd86-antibody-c86-1146-ab220188--immunocytochemistry-img13364.jpg "Immunocytochemistry - Anti-CD86 antibody [C86/1146] (ab220188)")
Immunocytochemistry - Anti-CD86 antibody [C86/1146] (ab220188)
Immunofluorescence analysis of Raji (Human Burkitt's lymphoma cell line) cells labeling CD86 with ab220188 at 2 µg/mL followed by Goat anti-Mouse IgG-CF488 (green). The nuclear counterstain is Reddot (red).
![Western blot - Anti-CD86 antibody [C86/1146] (ab220188), expandable thumbnail](https://content.abcam.com/products/images/cd86-antibody-c86-1146-ab220188--western-blot-img170813.jpg "Western blot - Anti-CD86 antibody [C86/1146] (ab220188)")
Western blot - Anti-CD86 antibody [C86/1146] (ab220188)
Lanes 1 - 4: Merged signal (red and green). Green - ab220188 observed at 70 kDa. Red - loading control Anti-alpha Tubulin antibody [EP1332Y] - Loading Control ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) observed at 55 kDa.ab220188 was shown to react with CD86 in wild-type Raji cells in Western blot with loss of signal observed in CD86 knockout cell line Human CD86 knockout Raji cell lysate ab273812 (knockout cell lysate Human CD86 knockout Raji cell line ab273858). Wild-type Raji and CD86 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab220188 and Anti-alpha Tubulin antibody [EP1332Y] - Loading Control ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) overnight at 4 °C at 0.5 μg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
All lanes: Western blot - Anti-CD86 antibody [C86/1146] (ab220188) at 0.5 µg/mL
Lane 1: Wild-type Raji cell lysate at 20 µg
Lane 2: CD86 knockout Raji cell lysate at 20 µg
Lane 2: Western blot - Human CD86 knockout Raji cell line (Human CD86 knockout Raji cell line ab273858)
Lane 3: Ramos cell lysate at 20 µg
Lane 4: Human Liver tissue lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 38 kDa
Observed band size: 70 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD86 antibody [C86/1146] (ab220188), expandable thumbnail](https://content.abcam.com/products/images/cd86-antibody-c86-1146-ab220188--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img18986.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD86 antibody [C86/1146] (ab220188)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD86 antibody [C86/1146] (ab220188)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labelling CD86 with ab220188 at 2 μg/mL for 30 minutes at room temperature. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at room temperature for 20 minutes.
![Western blot - Anti-CD86 antibody [C86/1146] (ab220188), expandable thumbnail](https://content.abcam.com/products/images/cd86-antibody-c86-1146-ab220188--western-blot-img35456.jpg "Western blot - Anti-CD86 antibody [C86/1146] (ab220188)")
Western blot - Anti-CD86 antibody [C86/1146] (ab220188)
All lanes: Western blot - Anti-CD86 antibody [C86/1146] (ab220188) at 1 µg
All lanes: Daudi cell lysate
Predicted band size: 38 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com