Anti-CD86 antibody [C86/1146] - BSA and Azide free

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD86 antibody [C86/1146] - BSA and Azide free (AB213045)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human melanoma tissue labeling CD86 with ab220188 at 4 μg/ml.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab220188)

• ICC

Supplier Data

Immunocytochemistry - Anti-CD86 antibody [C86/1146] - BSA and Azide free (AB213045)

This image was produced using ab220188, the same clone but in a different formulation.

Confocal Immunofluorescence image of Raji cells labeling CD86 with ab220188 followed by goat anti-Mouse IgG conjugated with CF488 (green). Red Dot is used to label the Nuclei (red).

• WB

Supplier Data

Western blot - Anti-CD86 antibody [C86/1146] - BSA and Azide free (AB213045)

CD86 is observed as a 70-kDa glycoprotein in Western blot.

This image was produced using ab220188, the same clone but in a different formulation.

All lanes:

Western blot - Anti-CD86 antibody [C86/1146] - BSA and Azide free (ab213045) at 2 µg/mL

All lanes:

Daudi (human Burkitt's lymphoma cell line) cell lysate

Predicted band size: 38 kDa

Observed band size: 70 kDa

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• WB

Lab

Western blot - Anti-CD86 antibody [C86/1146] - BSA and Azide free (AB213045)

Lanes 1 - 4 : Merged signal (red and green). Green - ab220188 observed at 70 kDa. Red - loading control ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) observed at 55 kDa.

ab220188 was shown to react with CD86 in wild-type Raji cells in Western blot with loss of signal observed in CD86 knockout cell line ab273812 (knockout cell lysate ab273858). Wild-type Raji and CD86 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween^®) before incubation with ab220188 and ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) overnight at 4 °C at 0.5 μg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye^® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye^® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-CD86 antibody [C86/1146] - BSA and Azide free (ab213045) at 0.5 µg/mL

Lane 1:

Wild-type Raji cell lysate at 20 µg

Lane 2:

CD86 knockout Raji cell lysate at 20 µg

Lane 3:

Ramos cell lysate at 20 µg

Lane 4:

Human Liver tissue lysate at 20 µg

Predicted band size: 38 kDa

Observed band size: 70 kDa

false