AB234401

Anti-CD86 antibody [C86/2160R]

Name: Anti-CD86 antibody [C86/2160R] (ab234401) | Abcam
Brand: Abcam Limited
SKU: AB234401
Price: 560 USD
Availability: InStock
Rating: 4 (2 reviews)

Recombinant
What is this?

(2 Reviews)

(10 Publications)

Rabbit Recombinant Monoclonal CD86 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 10 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CD86.

View Alternative Names

CD86, CD28LG2, T-lymphocyte activation antigen CD86, Activation B7-2 antigen, B70, BU63, CTLA-4 counter-receptor B7.2, FUN-1

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD86 antibody [C86/2160R] (AB234401)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD86 antibody [C86/2160R] (AB234401)

Formalin-fixed, paraffin-embedded human colon tissue stained for CD86 with ab234401 at 4 μg/ml in immunohistochemical analysis.

Carrier free

Anti-CD86 antibody [C86/2160R] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

C86/2160R

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human CD86.

P42081

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2-4 µg/mL", "IHCP-species-notes": "<p>Incubate with primary antibody for 30 minutes at room temperature.</p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Purification notes

Purified from bioreactor concentrate by Protein A/G.

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD86 also known as B7-2 is a protein involved in the regulation of the immune response. It has an approximate mass of 70 kDa and is expressed on antigen-presenting cells like dendritic cells monocytes and macrophages. Notably CD86 is present on macrophages including those in tissues such as skin and lymphoid organs. Expressed on these cells CD86 serves as a vital mediator in the co-stimulatory signals necessary for T cell activation and survival.

Biological function summary

CD86 plays a significant role in the immune system by providing secondary signals for T cell activation and differentiation. It is a part of the B7 protein family and forms a complex with CD28 and CTLA-4 on T cells. When CD86 binds to CD28 it sends positive co-stimulatory signals which promote T cell proliferation and cytokine production. On the other hand interaction with CTLA-4 transmits an inhibitory signal which reduces immune response. This dual interaction helps to balance immune activation and tolerance.

Pathways

CD86 takes part in important immune-related signaling pathways particularly the T cell receptor signaling pathway and the PI3K-Akt signaling pathway. Both pathways are fundamental for initiating immune responses. CD86's interaction with CD28 activates downstream signaling cascades including PI3K-Akt which is important for cell survival and growth. Additionally CD86 collaborates with other proteins such as CD80 another co-stimulatory molecule to amplify T cell activation within these pathways.

CD86 is associated with autoimmune diseases and transplant rejection. In autoimmune diseases like rheumatoid arthritis the overexpression or dysregulation of CD86 can lead to excessive T cell activation causing immune system attacks on the body's own tissues. Similarly in transplant rejection CD86 may contribute by enhancing immune response against transplanted organs. The engagement between CD86 and CD28 is a critical factor in these conditions and therapies targeting this interaction are under exploration to mitigate the immune response.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Costimulatory molecule that belongs to the immunoglobulin superfamily that plays an important role in T-lymphocyte activation (PubMed : 12196291, PubMed : 7694363). Acts as the primary auxiliary signal augmenting the MHC/TCR signal in naive T-cells by acting as a ligand for the CD28 receptor which is constitutively expressed on the cell surface of T-cells (PubMed : 12196291, PubMed : 7694363). May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation (PubMed : 7527824). Also involved in the regulation of B cells function, plays a role in regulating the level of IgG(1) produced. Upon CD40 engagement, activates NF-kappa-B signaling pathway via phospholipase C and protein kinase C activation (By similarity). Also acts as an inhibitor of T-cell activation by acting as a ligand for CTLA4, a decoy receptor, thereby blocking CD28-mediated T-cell priming (PubMed : 11279501).. Isoform 2. Interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.. (Microbial infection) Acts as a receptor for adenovirus subgroup B.

See full target information CD86

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Acta clinica Croatica 63:684-692 PubMed41050242

2025

RELATIONSHIP OF IMMUNOHISTOCHEMICAL EXPRESSION OF CD86/CD163 POSITIVE INTRATUMORAL MACROPHAGES WITH PROGNOSIS OF PANCREATIC DUCTAL ADENOCARCINOMA.

Applications

Unspecified application

Species

Unspecified reactive species

Goran Glavčić,Mario Zovak,Slavica Sović,Silvija Mašić,Nina Blažević,Zvonimir Misir,Zdenko Bilić,Marin Glavčić,Petra Radulović

Infectious agents and cancer 20:36 PubMed40514669

2025

DNA hypomethylation modification promotes BST2 expression in cervical cancer by facilitating STAT1 binding to the promoter of BST2.

Applications

Unspecified application

Species

Unspecified reactive species

Reziwanguli Wubuli,Zumurelaiti Ainiwaer,Mayinuer Niyazi,Lili Han

BMC gastroenterology 24:417 PubMed39567902

2024

Correlation between PDGF-BB and M1-type macrophage in inflammatory bowel disease: a case-control study.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiyun Fang,Siwen Qu,Xia Ji,Chuwei Zheng,Juanfen Mo,Jianqiu Xu,Jinming Zhang,Haiyan Shen

Acta biomaterialia 191:189-204 PubMed39532649

2024

Harnessing synergistic effects of MMP-2 Inhibition and bFGF to simultaneously preserve and vascularize cardiac extracellular matrix after myocardial infarction.

Applications

Unspecified application

Species

Unspecified reactive species

Hong Niu,Zhongting Liu,Ya Guan,Jiaxing Wen,Yu Dang,Jianjun Guan

Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas 57:e13116 PubMed39383377

2024

Amelioration of nephrotoxicity by targeting ferroptosis: role of NCOA4, IREB2, and SLC7a11 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

N Sharawy,B E Aboulhoda,M M Khalifa,G N Morcos,S A A G Morsy,M A Alghamdi,I M Khalifa,W A Abd Algaleel

Advanced materials (Deerfield Beach, Fla.) 36:e2304333 PubMed38096399

2023

Inflammation-Targeting Mesenchymal Stem Cells Combined with Photothermal Treatment Attenuate Severe Joint Inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Min Jun Shin,Jun-Young Park,Jun Young Park,Su Hyun Lim,Hyoungsub Lim,Jin Kyeong Choi,Chul-Kyu Park,Youn Joo Kang,Dongwoo Khang

Molecular and cellular biochemistry 478:1611-1620 PubMed36441354

2022

(-)-Guaiol triggers immunogenic cell death and inhibits tumor growth in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaohui Yang,Junling Yang,Xiaoxia Gu,Yuhua Tao,Hongjuan Ji,Xian Miao,Shuijie Shen,Haiyang Zang

International journal of oral science 14:44 PubMed36064768

2022

Dental stem cell-derived extracellular vesicles transfer miR-330-5p to treat traumatic brain injury by regulating microglia polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Ye Li,Meng Sun,Xinxin Wang,Xiaoyu Cao,Na Li,Dandan Pei,Ang Li

Science advances 7: PubMed34452918

2021

Sustained oxygenation accelerates diabetic wound healing by promoting epithelialization and angiogenesis and decreasing inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Ya Guan,Hong Niu,Zhongting Liu,Yu Dang,Jie Shen,Mohamed Zayed,Liang Ma,Jianjun Guan

International journal of molecular sciences 22: PubMed33401449

2021

The Paradox Effect of Calcification in Carotid Atherosclerosis: Microcalcification is Correlated with Plaque Instability.

Applications

Unspecified application

Species

Unspecified reactive species

Manuela Montanaro,Manuel Scimeca,Lucia Anemona,Francesca Servadei,Erica Giacobbi,Rita Bonfiglio,Elena Bonanno,Nicoletta Urbano,Arnaldo Ippoliti,Giuseppe Santeusanio,Orazio Schillaci,Alessandro Mauriello

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD86 antibody [C86/2160R]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD86 antibody [C86/2160R] (AB234401)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (10)

Product promise