JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB242142

Anti-CD86 antibody [EPR22958-106]

Be the first to review this product! Submit a review

|

(8 Publications)

Rabbit Recombinant Monoclonal CD86 antibody. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 8 publications.

View Alternative Names

CD86, T-lymphocyte activation antigen CD86, Activation B7-2 antigen, Early T-cell costimulatory molecule 1, ETC-1, Cd86

2 Images
Flow Cytometry - Anti-CD86 antibody [EPR22958-106] (AB242142)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-CD86 antibody [EPR22958-106] (AB242142)

Flow cytometric analysis of Mouse splenocytes (treated with 1 ug/ml lipopolysaccharide (LPS) for 3 days) labelling CD86 with ab242142 at 1/600 (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody.

Cells were stained with rabbit IgG (Left) or ab242142 (Right). Then stained with anti-CD19 conjugated to Alexa Fluor® 647.
Gated on viable cells.

Flow Cytometry - Anti-CD86 antibody [EPR22958-106] (AB242142)
  • Flow Cyt

Lab

Flow Cytometry - Anti-CD86 antibody [EPR22958-106] (AB242142)

Flow cytometry overlay histogram showing mouse splenocytes treated with 1µg/ml LPS for 3 days (bottom) and negative untreated mouse splenocytes (top). Cells were stained with ab242142 (right) or Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control used at the same concentration and conditions as the primary antibody. The cells were incubated in 1x PBS containing 10% mouse serum and 10 % normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab242142 (1x 106 in 100μl at 0.2 μg/ml (1/10,500)) for 30min on ice. The cells were simultaneously stained with CD19.

The secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed was incubated at 1/2000 for 30min on ice.

Acquisition of >30000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.

  • 578 PE

    PE Anti-CD86 antibody [EPR22958-106]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-CD86 antibody [EPR22958-106]

  • Carrier free

    Anti-CD86 antibody [EPR22958-106] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22958-106

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/600", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD86 also known as B7-2 is a protein involved in the regulation of the immune response. It has an approximate mass of 70 kDa and is expressed on antigen-presenting cells like dendritic cells monocytes and macrophages. Notably CD86 is present on macrophages including those in tissues such as skin and lymphoid organs. Expressed on these cells CD86 serves as a vital mediator in the co-stimulatory signals necessary for T cell activation and survival.
Biological function summary

CD86 plays a significant role in the immune system by providing secondary signals for T cell activation and differentiation. It is a part of the B7 protein family and forms a complex with CD28 and CTLA-4 on T cells. When CD86 binds to CD28 it sends positive co-stimulatory signals which promote T cell proliferation and cytokine production. On the other hand interaction with CTLA-4 transmits an inhibitory signal which reduces immune response. This dual interaction helps to balance immune activation and tolerance.

Pathways

CD86 takes part in important immune-related signaling pathways particularly the T cell receptor signaling pathway and the PI3K-Akt signaling pathway. Both pathways are fundamental for initiating immune responses. CD86's interaction with CD28 activates downstream signaling cascades including PI3K-Akt which is important for cell survival and growth. Additionally CD86 collaborates with other proteins such as CD80 another co-stimulatory molecule to amplify T cell activation within these pathways.

CD86 is associated with autoimmune diseases and transplant rejection. In autoimmune diseases like rheumatoid arthritis the overexpression or dysregulation of CD86 can lead to excessive T cell activation causing immune system attacks on the body's own tissues. Similarly in transplant rejection CD86 may contribute by enhancing immune response against transplanted organs. The engagement between CD86 and CD28 is a critical factor in these conditions and therapies targeting this interaction are under exploration to mitigate the immune response.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Also involved in the regulation of B cells function, plays a role in regulating the level of IgG(1) produced. Upon CD40 engagement, activates NF-kappa-B signaling pathway via phospholipase C and protein kinase C activation (PubMed : 23241883).
See full target information Cd86
style
left-column

Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Materials today. Bio 33:102045 PubMed40688666

2025

Dendritic cell membrane-based DCsLipo@MnO@siCTLA4@PD-1α nanomedicine for the treatment of Lynch syndrome-related colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjin Zhong,Pengcheng Wang,Jintian Wang,Yuejia Zhu,Liquan Chen,Yilong Qian,Xiang Huang,Kai Ye

IBRO neuroscience reports 18:39-49 PubMed39816480

2025

Postoperative analgesia with morphine promoting microglial activation and neuroinflammation induced by surgery aggravates perioperative neurocognitive dysfunction in aged mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xiuzhi Shao,Liping Xie,Jingwen Zhai,Mingyue Ge

Frontiers in cell and developmental biology 12:1368021 PubMed38596358

2024

Deciphering perivascular macrophages and microglia in the retinal ganglion cell layers.

Applications

Unspecified application

Species

Unspecified reactive species

Jehwi Jeon,Yong Soo Park,Sang-Hoon Kim,Eunji Kong,Jay Kim,Jee Myung Yang,Joo Yong Lee,You-Me Kim,In-Beom Kim,Pilhan Kim

Journal of inflammation research 17:1267-1279 PubMed38434584

2024

BAP31 Promotes Adhesion Between Endothelial Cells and Macrophages Through the NF-κB Signaling Pathway in Sepsis.

Applications

Unspecified application

Species

Unspecified reactive species

Jiawei He,Danyang Jing,Shen Zhao,Meili Duan

Journal of advanced research 44:213-225 PubMed36725191

2023

Lung specific homing of diphenyleneiodonium chloride improves pulmonary fibrosis by inhibiting macrophage M2 metabolic program.

Applications

Unspecified application

Species

Unspecified reactive species

Huirui Wang,Yinghui Gao,Li Wang,Yang Yu,Jiaozhen Zhang,Chunyu Liu,Yaxin Song,Haochuan Xu,Jingcheng Wang,Hongxiang Lou,Ting Dong

Frontiers in medicine 9:897800 PubMed35911406

2022

Establishment of the reproducible branch retinal artery occlusion mouse model and intravital longitudinal imaging of the retinal CX3CR1-GFP cells after spontaneous arterial recanalization.

Applications

Unspecified application

Species

Unspecified reactive species

Jehwi Jeon,Sang-Hoon Kim,Eunji Kong,Soo Jin Kim,Jee Myung Yang,Joo Yong Lee,Junyeop Lee,You-Me Kim,Pilhan Kim

Frontiers in pharmacology 12:719823 PubMed34744713

2021

Phillyrin Prevents Neuroinflammation-Induced Blood-Brain Barrier Damage Following Traumatic Brain Injury Altering Microglial Polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Jiang,Ding Wei,Xuejun He,Chao Gan,Xiaobing Long,Huaqiu Zhang

Annals of translational medicine 8:1151 PubMed33241000

2020

Dihydromyricetin attenuates neuropathic pain via enhancing the transition from M1 to M2 phenotype polarization by potentially elevating ALDH2 activity and .

Applications

Unspecified application

Species

Unspecified reactive species

Wei Zhang,Lingxiao Yang,Longyun Li,Wei Feng
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB213045

Anti-CD86 antibody [C86/1146] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD86 antibody [C86/1146] - BSA and Azide free (AB213045)

  • 0
  • 0
Primary Antibodies

AB243886

Anti-CD86 antibody [BLR029F] - BSA free

Recombinant

Immunocytochemistry - Anti-CD86 antibody [BLR029F] - BSA free (AB243886)

  • 0
  • 0
Primary Antibodies

AB256270

Alexa Fluor® 488 Anti-CD86 antibody [OX48]

Flow Cytometry - Alexa Fluor® 488 Anti-CD86 antibody [OX48] (AB256270)

  • 0
  • 0
Primary Antibodies

AB256271

Alexa Fluor® 647 Anti-CD86 antibody [OX48]

Flow Cytometry - Alexa Fluor® 647 Anti-CD86 antibody [OX48] (AB256271)

  • 0
  • 0
Primary Antibodies

AB242020

Anti-CD86 antibody [EPR21962] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Flow Cytometry - Anti-CD86 antibody [EPR21962] - BSA and Azide free (AB242020)

  • 0
  • 0
Primary Antibodies

AB239075

Anti-CD86 antibody [EPR21962]

20ul selling size|RabMAb|Recombinant|KO Validated

Flow Cytometry - Anti-CD86 antibody [EPR21962] (AB239075)

  • 0
  • 0
Primary Antibodies

AB243887

Anti-CD86 antibody [BLR030F] - BSA free

Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD86 antibody [BLR030F] - BSA free (AB243887)

  • 0
  • 0

Complementary Products

Assay Kits

AB315052

Mouse CD86 ELISA Kit

SimpleStep

Sandwich ELISA - Mouse CD86 ELISA Kit (AB315052)

  • 0
  • 0
Primary Antibodies

AB64693

Anti-Mannose Receptor antibody

BOND RX™ Validated

Western blot - Anti-Mannose Receptor antibody (AB64693)

  • 5
  • 42
Primary Antibodies

AB15323

Anti-iNOS antibody

BOND RX™ Validated

Western blot - Anti-iNOS antibody (AB15323)

  • 4
  • 20
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB182422

Anti-CD163 antibody [EPR19518]

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant

Flow Cytometry - Anti-CD163 antibody [EPR19518] (AB182422)

  • 5
  • 17

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com