Rabbit Recombinant Monoclonal CD86 antibody. Carrier free. Suitable for Flow Cyt and reacts with Mouse samples.
Images
![Flow Cytometry - Anti-CD86 antibody [EPR22958-106] - BSA and Azide free (AB263872), expandable thumbnail](https://content.abcam.com/products/images/cd86-antibody-epr22958-106-bsa-and-azide-free-ab263872--flow-cytometry-img37387.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CD86 antibody [EPR22958-106] - BSA and Azide free (AB263872)")
![Flow Cytometry - Anti-CD86 antibody [EPR22958-106] - BSA and Azide free (AB263872), expandable thumbnail](https://content.abcam.com/products/images/cd86-antibody-epr22958-106-bsa-and-azide-free-ab263872--flow-cytometry-img440955.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CD86 antibody [EPR22958-106] - BSA and Azide free (AB263872)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|---|
| Mouse | Not recommended | Tested | Not recommended | Not recommended | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
2 products for Alternative Version
Target data
Function
Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Also involved in the regulation of B cells function, plays a role in regulating the level of IgG(1) produced. Upon CD40 engagement, activates NF-kappa-B signaling pathway via phospholipase C and protein kinase C activation (PubMed:23241883).
Alternative names
CD86, T-lymphocyte activation antigen CD86, Activation B7-2 antigen, Early T-cell costimulatory molecule 1, ETC-1, Cd86
Recommended products
Rabbit Recombinant Monoclonal CD86 antibody. Carrier free. Suitable for Flow Cyt and reacts with Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR22958-106
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab263872 is the carrier-free version of Anti-CD86 antibody [EPR22958-106] ab242142.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD86 also known as B7-2 is a protein involved in the regulation of the immune response. It has an approximate mass of 70 kDa and is expressed on antigen-presenting cells like dendritic cells monocytes and macrophages. Notably CD86 is present on macrophages including those in tissues such as skin and lymphoid organs. Expressed on these cells CD86 serves as a vital mediator in the co-stimulatory signals necessary for T cell activation and survival.
- Biological function summary
CD86 plays a significant role in the immune system by providing secondary signals for T cell activation and differentiation. It is a part of the B7 protein family and forms a complex with CD28 and CTLA-4 on T cells. When CD86 binds to CD28 it sends positive co-stimulatory signals which promote T cell proliferation and cytokine production. On the other hand interaction with CTLA-4 transmits an inhibitory signal which reduces immune response. This dual interaction helps to balance immune activation and tolerance.
- Pathways
CD86 takes part in important immune-related signaling pathways particularly the T cell receptor signaling pathway and the PI3K-Akt signaling pathway. Both pathways are fundamental for initiating immune responses. CD86's interaction with CD28 activates downstream signaling cascades including PI3K-Akt which is important for cell survival and growth. Additionally CD86 collaborates with other proteins such as CD80 another co-stimulatory molecule to amplify T cell activation within these pathways.
- Associated diseases and disorders
CD86 is associated with autoimmune diseases and transplant rejection. In autoimmune diseases like rheumatoid arthritis the overexpression or dysregulation of CD86 can lead to excessive T cell activation causing immune system attacks on the body's own tissues. Similarly in transplant rejection CD86 may contribute by enhancing immune response against transplanted organs. The engagement between CD86 and CD28 is a critical factor in these conditions and therapies targeting this interaction are under exploration to mitigate the immune response.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Flow Cytometry - Anti-CD86 antibody [EPR22958-106] - BSA and Azide free (ab263872), expandable thumbnail](https://content.abcam.com/products/images/cd86-antibody-epr22958-106-bsa-and-azide-free-ab263872--flow-cytometry-img37387.jpg "Flow Cytometry - Anti-CD86 antibody [EPR22958-106] - BSA and Azide free (ab263872)")
Flow Cytometry - Anti-CD86 antibody [EPR22958-106] - BSA and Azide free (ab263872)
Flow cytometric analysis of Mouse splenocytes (treated with 1 ug/ml lipopolysaccharide (LPS) for 3 days) labelling CD86 with Anti-CD86 antibody [EPR22958-106] ab242142 at 1/600 (Right) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) isotype control (Left). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 was used as the secondary antibody.
Cells were stained with rabbit IgG (Left) or Anti-CD86 antibody [EPR22958-106] ab242142 (Right). Then stained with anti-CD19 conjugated to Alexa Fluor® 647.
Gated on viable cells.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD86 antibody [EPR22958-106] ab242142).
![Flow Cytometry - Anti-CD86 antibody [EPR22958-106] - BSA and Azide free (ab263872), expandable thumbnail](https://content.abcam.com/products/images/cd86-antibody-epr22958-106-bsa-and-azide-free-ab263872--flow-cytometry-img440955.jpg "Flow Cytometry - Anti-CD86 antibody [EPR22958-106] - BSA and Azide free (ab263872)")
Flow Cytometry - Anti-CD86 antibody [EPR22958-106] - BSA and Azide free (ab263872)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD86 antibody [EPR22958-106] ab242142).
Flow cytometry overlay histogram showing mouse splenocytes treated with LPS, 3 Days, 1 µg/ml (bottom) and negative untreated mouse splenocytes (top) stained with Anti-CD86 antibody [EPR22958-106] ab242142 (right) or Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control used at the same concentration and conditions as the primary antibody. The cells were incubated in 1x PBS containing 10% mouse serum and 10 % normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody Anti-CD86 antibody [EPR22958-106] ab242142 (1x 106 in 100μl at 0.2 μg/ml (1/10,500)) for 30min on ice. The cells were simultaneously stained with CD19.
The secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed was incubated at 1/2000 for 30min on ice.
Acquisition of >30000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.
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