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Knockout Tested Rabbit Recombinant Monoclonal CD86 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.

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Images

Western blot - Anti-CD86 antibody [EPR28721-30] - BSA and Azide free (AB317267), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD86 antibody [EPR28721-30] - BSA and Azide free (AB317267), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD86 antibody [EPR28721-30] - BSA and Azide free (AB317267), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD86 antibody [EPR28721-30] - BSA and Azide free (AB317267), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD86 antibody [EPR28721-30] - BSA and Azide free (AB317267), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PIPFlow CytICC/IF
Human
Tested
Tested
Not recommended
Not recommended
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 products for Alternative Version

    Target data

    Function

    Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation (PubMed:7527824). Also involved in the regulation of B cells function, plays a role in regulating the level of IgG(1) produced. Upon CD40 engagement, activates NF-kappa-B signaling pathway via phospholipase C and protein kinase C activation (By similarity).Isoform 2Interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.(Microbial infection) Acts as a receptor for adenovirus subgroup B.

    Alternative names

    T-lymphocyte activation antigen CD86, Activation B7-2 antigen, B70, BU63, CTLA-4 counter-receptor B7.2, FUN-1, CD28LG2, CD86

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    Knockout Tested Rabbit Recombinant Monoclonal CD86 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.

    Alternative names

    T-lymphocyte activation antigen CD86, Activation B7-2 antigen, B70, BU63, CTLA-4 counter-receptor B7.2, FUN-1, CD28LG2, CD86

    Key facts

    Isotype

    IgG

    Form

    Liquid

    Clonality

    Monoclonal

    Immunogen
    • The exact immunogen used to generate this antibody is proprietary information.
    Carrier free

    Yes

    Clone number

    EPR28721-30

    Purification technique

    Affinity purification Protein A

    Concentration
    Loading...

    Storage

    Shipped at conditions

    Blue Ice

    Appropriate long-term storage conditions

    +4°C

    Notes

    ab317267 is the carrier-free version of Anti-CD86 antibody [EPR28721-30] ab317266.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free batch production

    For more information, read more on recombinant antibodies.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    Supplementary info

    Activity summary

    CD86 also known as B7-2 is a protein involved in the regulation of the immune response. It has an approximate mass of 70 kDa and is expressed on antigen-presenting cells like dendritic cells monocytes and macrophages. Notably CD86 is present on macrophages including those in tissues such as skin and lymphoid organs. Expressed on these cells CD86 serves as a vital mediator in the co-stimulatory signals necessary for T cell activation and survival.

    Biological function summary

    CD86 plays a significant role in the immune system by providing secondary signals for T cell activation and differentiation. It is a part of the B7 protein family and forms a complex with CD28 and CTLA-4 on T cells. When CD86 binds to CD28 it sends positive co-stimulatory signals which promote T cell proliferation and cytokine production. On the other hand interaction with CTLA-4 transmits an inhibitory signal which reduces immune response. This dual interaction helps to balance immune activation and tolerance.

    Pathways

    CD86 takes part in important immune-related signaling pathways particularly the T cell receptor signaling pathway and the PI3K-Akt signaling pathway. Both pathways are fundamental for initiating immune responses. CD86's interaction with CD28 activates downstream signaling cascades including PI3K-Akt which is important for cell survival and growth. Additionally CD86 collaborates with other proteins such as CD80 another co-stimulatory molecule to amplify T cell activation within these pathways.

    Associated diseases and disorders

    CD86 is associated with autoimmune diseases and transplant rejection. In autoimmune diseases like rheumatoid arthritis the overexpression or dysregulation of CD86 can lead to excessive T cell activation causing immune system attacks on the body's own tissues. Similarly in transplant rejection CD86 may contribute by enhancing immune response against transplanted organs. The engagement between CD86 and CD28 is a critical factor in these conditions and therapies targeting this interaction are under exploration to mitigate the immune response.

    Product promise

    We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

    In the unlikely event of one of our products not working as expected, you are covered by our product promise.

    Full details and terms and conditions can be found here:
    Terms & Conditions.

    7 product images

    • Western blot - Anti-CD86 antibody [EPR28721-30] - BSA and Azide free (ab317267), expandable thumbnail

      Western blot - Anti-CD86 antibody [EPR28721-30] - BSA and Azide free (ab317267)

      This data was developed using Anti-CD86 antibody [EPR28721-30] ab317266, the same antibody clone in a different buffer formulation.

      Negative control: HT-29.

      In Western blot, Anti-CD86 antibody [EPR28721-30] ab317266 was shown to bind specifically to CD86. Target of interest was observed at 70 kDa in wild-type Raji cell lysates (lane 4) with no signal observed at this size in CD86 knockout cell line (lane 5) (lane 5, knockout cell line Human CD86 knockout Raji cell line ab273858 / knockout cell lysate Human CD86 knockout Raji cell lysate ab273812).

      In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

      Exposure time: Lane 1: 15 seconds, lanes 2-5: 180 seconds

      All lanes: Western blot - Anti-CD86 antibody [EPR28721-30] (Anti-CD86 antibody [EPR28721-30] ab317266) at 1/1000 dilution

      Lane 1: HDLM-2 (human Hodgkin lymphoma) whole cell lysate at 20 µg

      Lane 2: Ramos (human Burkitts lymphoma B lymphocyte) whole cell lysate at 20 µg

      Lane 3: HT-29 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

      Lane 4: Wild-type Raji (human Burkitts lymphoma B lymphocyte) whole cell lysate at 20 µg

      Lane 5: CD86 knockout Raji whole cell lysate at 20 µg

      Secondary

      All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

      Performed under reducing conditions.

      Observed band size: 70 kDa, 36 kDa

      Negative control: HT-29.

      In Western blot, Anti-CD86 antibody [EPR28721-30] ab317266 was shown to bind specifically to CD86. Target of interest was observed at 70 kDa in wild-type Raji cell lysates (lane 4) with no signal observed at this size in CD86 knockout cell line (lane 5) (lane 5, knockout cell line Human CD86 knockout Raji cell line ab273858 / knockout cell lysate Human CD86 knockout Raji cell lysate ab273812).

      In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

      Exposure time: Lane 1: 15 seconds, lanes 2-5: 180 seconds

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD86 antibody [EPR28721-30] - BSA and Azide free (ab317267), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD86 antibody [EPR28721-30] - BSA and Azide free (ab317267)

      This data was developed using Anti-CD86 antibody [EPR28721-30] ab317266, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling CD86 with Anti-CD86 antibody [EPR28721-30] ab317266 at 1/1000 (0.513 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Negative control: No staining on human skeletal muscle.

      The section was incubated with Anti-CD86 antibody [EPR28721-30] ab317266 for 30 mins at room temperature.

      The immunostaining was performed on a Leica Biosystems BOND® RX instrument

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD86 antibody [EPR28721-30] - BSA and Azide free (ab317267), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD86 antibody [EPR28721-30] - BSA and Azide free (ab317267)

      This data was developed using Anti-CD86 antibody [EPR28721-30] ab317266, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded (A)HDLM-2 (human Hodgkin lymphoma cell). (B)HT-29 (human colorectal adenocarcinoma epithelial cell). tissue labeling CD86 with Anti-CD86 antibody [EPR28721-30] ab317266 at 1/1000 (0.513 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Positive staining on (A)HDLM-2, no staining on (B) HT-29.

      The section was incubated with Anti-CD86 antibody [EPR28721-30] ab317266 for 30 mins at room temperature.

      The immunostaining was performed on a Leica Biosystems BOND® RX instrument

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD86 antibody [EPR28721-30] - BSA and Azide free (ab317267), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD86 antibody [EPR28721-30] - BSA and Azide free (ab317267)

      This data was developed using Anti-CD86 antibody [EPR28721-30] ab317266, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded (A)Wild-type Raji. (B)CD86 knockout Raji (human Burkitt's lymphoma B lymphocyte). tissue labeling CD86 with Anti-CD86 antibody [EPR28721-30] ab317266 at 1/1000 (0.513 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Positive staining on (A) Wild-type Raji, no staining on (B) CD86 knockout Raji.

      The section was incubated with Anti-CD86 antibody [EPR28721-30] ab317266 for 30 mins at room temperature.

      The immunostaining was performed on a Leica Biosystems BOND® RX instrument

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD86 antibody [EPR28721-30] - BSA and Azide free (ab317267), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD86 antibody [EPR28721-30] - BSA and Azide free (ab317267)

      This data was developed using Anti-CD86 antibody [EPR28721-30] ab317266, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling CD86 with Anti-CD86 antibody [EPR28721-30] ab317266 at 1/1000 (0.513 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Positive staining on human lung carcinoma.

      The section was incubated with Anti-CD86 antibody [EPR28721-30] ab317266 for 30 mins at room temperature.

      The immunostaining was performed on a Leica Biosystems BOND® RX instrument

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD86 antibody [EPR28721-30] - BSA and Azide free (ab317267), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD86 antibody [EPR28721-30] - BSA and Azide free (ab317267)

      This data was developed using Anti-CD86 antibody [EPR28721-30] ab317266, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling CD86 with Anti-CD86 antibody [EPR28721-30] ab317266 at 1/1000 (0.513 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Positive staining on human tonsil.

      The section was incubated with Anti-CD86 antibody [EPR28721-30] ab317266 for 30 mins at room temperature.

      The immunostaining was performed on a Leica Biosystems BOND® RX instrument

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD86 antibody [EPR28721-30] - BSA and Azide free (ab317267), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD86 antibody [EPR28721-30] - BSA and Azide free (ab317267)

      This data was developed using Anti-CD86 antibody [EPR28721-30] ab317266, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling CD86 with Anti-CD86 antibody [EPR28721-30] ab317266 at 1/1000 (0.513 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Positive staining on immune cells of human lung.

      The section was incubated with Anti-CD86 antibody [EPR28721-30] ab317266 for 30 mins at room temperature.

      The immunostaining was performed on a Leica Biosystems BOND® RX instrument

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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