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AB119857

Anti-CD86 antibody [GL-1]

1

(2 Reviews)

|

(67 Publications)

Anti-CD86 antibody [GL-1] (ab119857) is a rat monoclonal antibody detecting CD86 in Flow Cytometry, IP, ICC/IF, FuncS, BL. Suitable for Mouse.

- Over 30 publications

View Alternative Names

CD86, T-lymphocyte activation antigen CD86, Activation B7-2 antigen, Early T-cell costimulatory molecule 1, ETC-1, Cd86

  • 519 FITC

    FITC Anti-CD86 antibody [GL-1]

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

GL-1

Isotype

IgG2a

Carrier free

No

Reacts with

Mouse

Applications

ICC/IF, Flow Cyt, IP

applications

Immunogen

Cell preparation containing Cd86 protein. The exact immunogen used to generate this antibody is proprietary information.

P42082

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Reactivity data

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Product details

What is this antibody validated in?
Anti-CD86 antibody [GL-1] (ab119857) is a rat monoclonal antibody and is validated for use in Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunocytochemistry/immunofluorescence (ICC/IF), Functional Studies, Blocking in Mouse samples.

Trusted by the scientific community
Anti-CD86 [GL-1] (ab119857) was first used in a scientific publication in 2011 and has been cited over 30 times in peer-reviewed journals.

Other related products
We have a range of other formats of antibody clone [GL-1] also available for your convenience: ab119857, FITC - ab234237
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
This purified format is guaranteed to be >90% pure as determined by SDS-PAGE analysis.
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: 0.87% Sodium chloride, 0.14% Sodium dihydrogen phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze|Store undiluted
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD86 also known as B7-2 is a protein involved in the regulation of the immune response. It has an approximate mass of 70 kDa and is expressed on antigen-presenting cells like dendritic cells monocytes and macrophages. Notably CD86 is present on macrophages including those in tissues such as skin and lymphoid organs. Expressed on these cells CD86 serves as a vital mediator in the co-stimulatory signals necessary for T cell activation and survival.
Biological function summary

CD86 plays a significant role in the immune system by providing secondary signals for T cell activation and differentiation. It is a part of the B7 protein family and forms a complex with CD28 and CTLA-4 on T cells. When CD86 binds to CD28 it sends positive co-stimulatory signals which promote T cell proliferation and cytokine production. On the other hand interaction with CTLA-4 transmits an inhibitory signal which reduces immune response. This dual interaction helps to balance immune activation and tolerance.

Pathways

CD86 takes part in important immune-related signaling pathways particularly the T cell receptor signaling pathway and the PI3K-Akt signaling pathway. Both pathways are fundamental for initiating immune responses. CD86's interaction with CD28 activates downstream signaling cascades including PI3K-Akt which is important for cell survival and growth. Additionally CD86 collaborates with other proteins such as CD80 another co-stimulatory molecule to amplify T cell activation within these pathways.

CD86 is associated with autoimmune diseases and transplant rejection. In autoimmune diseases like rheumatoid arthritis the overexpression or dysregulation of CD86 can lead to excessive T cell activation causing immune system attacks on the body's own tissues. Similarly in transplant rejection CD86 may contribute by enhancing immune response against transplanted organs. The engagement between CD86 and CD28 is a critical factor in these conditions and therapies targeting this interaction are under exploration to mitigate the immune response.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Also involved in the regulation of B cells function, plays a role in regulating the level of IgG(1) produced. Upon CD40 engagement, activates NF-kappa-B signaling pathway via phospholipase C and protein kinase C activation (PubMed : 23241883).
See full target information Cd86
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Publications (67)

Recent publications for all applications. Explore the full list and refine your search

Dental materials journal 44:564-571 PubMed40958567

2025

Carbon nanohorn coating on titanium promotes M2 macrophage polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Sadahito Kimura,Eri Hirata,Yuta Takano,Miho Ibi,Natsumi Ushijima,Eijiro Miyako,Masako Yudasaka,Yukari Maeda,Daisuke Konishi,Kiwamu Sakaguchi,Atsuro Yokoyama

CNS neuroscience & therapeutics 31:e70606 PubMed40916543

2025

Neuroprotective Effects of Transcranial Pulsed Current Stimulation: Modulation of Microglial Polarization in Traumatic Brain Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Yao,Bingkai Ren,Qianhui Zhou,Yang Bai,Zhen Feng

Mediators of inflammation 2025:6149780 PubMed40832057

2025

Identification of Ifitm1 as a Pivotal Gene in Mouse Spinal Cord Injury Using Comprehensive Machine Learning Algorithms.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Yao,Kun Xi,Shaohu Xu,Feiyu Zhang,Liang Chen

Materials today. Bio 34:102160 PubMed40822925

2025

Delivered baicalein immunomodulatory hydrogel with dual properties of pH-responsive and anti-infection orchestrates pro-regenerative response of macrophages for enhanced hypertrophic scars therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Fengqing Shang,Yanling Qu,Yu Li,Lingjuan Dong,Dan Liu,Zhe Wang,Afeng Li,Yinghui Li,Dan Zhang,Leiguo Ming,Ronghua Jin

Science advances 11:eadx3851 PubMed40802748

2025

Nanoscale acoustic oscillator for mechanoimmunology: NAOMI.

Applications

Unspecified application

Species

Unspecified reactive species

Ye He,Ke Jin,Bo Pan,Ke Li,John D H Mai,Xianchen Xu,Ying Chen,Zhiteng Ma,Kaichun Yang,Shujie Yang,Kilian Shambaugh,Mingyuan Liu,Jianping Xia,Yuqi Wu,Luke P Lee,Tony Jun Huang

Bioactive materials 53:507-521 PubMed40755848

2025

Natural-origin bioadhesive hydrogel with dual antioxidative and immunoregulatory properties for enhanced angiogenesis and wound healing.

Applications

Unspecified application

Species

Unspecified reactive species

Huiyang Li,Lifei Ma,Ni Zhu,Xiaoyu Liang,Kaijing Liu,Xue Fu,Chuangnian Zhang,Jing Yang

The journal of headache and pain 26:158 PubMed40640733

2025

Spi1 aggravates neuropathic pain by modulating Clec7a-mediated neuroinflammation and microglial phagocytosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yin Xu,Xinli Liu,Hui Chen,Yun Zhao,Yubai Zhao,Yu Xie,Jiahui Pang,Hui Zeng,Yanyan Zeng,Weiwei Peng,Manxu Zheng,Wen Wu

eLife 13: PubMed40536105

2025

exploits host- and bacterial-derived β-alanine for replication inside host macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Shuai Ma,Bin Yang,Yuyang Sun,Xinyue Wang,Houliang Guo,Ruiying Liu,Ting Ye,Chenbo Kang,Jingnan Chen,Lingyan Jiang

Nature communications 16:4032 PubMed40301389

2025

Transparent, metal-free PEDOT:PSS neural interfaces for simultaneous recording of low-noise electrophysiology and artifact-free two-photon imaging.

Applications

Unspecified application

Species

Unspecified reactive species

Hyun Woo Kim,Jiwon Kim,Jong Youl Kim,Kyubeen Kim,Ju Young Lee,Taemin Kim,Shinil Cho,Jong Bin An,Hyun Jae Kim,Lulu Sun,Sunghoon Lee,Kenjiro Fukuda,Takao Someya,Mingyu Sang,Young Uk Cho,Jong Eun Lee,Ki Jun Yu

European journal of medical research 30:96 PubMed39940010

2025

Catalpalactone protects rats nerve function from hypoxic lesion by polarizing microglial cells toward M2 phenotype.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Wang,Qi Wang,Xin Sui,Mingxing Guo,Li Li,Weiwei Jia,Yinan Tian,Qi Lu,Bo Wang
View all publications
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