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Mouse Monoclonal CD86 antibody. Carrier free. Suitable for Flow Cyt, IHC-Fr and reacts with Rat samples.

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Images

Flow Cytometry - Anti-CD86 antibody [OX48] - BSA and Azide free (AB244583), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-CD86 antibody [OX48] - BSA and Azide free (AB244583), expandable thumbnail

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer
Standard buffer
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytIHC-Fr
Rat
Tested
Tested

Tested
Tested

Species
Rat
Dilution info
5 µg/mL
Notes

-

Tested
Tested

Species
Rat
Dilution info
5 µg/mL
Notes

-

Associated Products

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1 product for Alternative Version

Target data

Function

Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4 (PubMed:12196291). May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation (PubMed:7527824). Also involved in the regulation of B cells function, plays a role in regulating the level of IgG(1) produced. Upon CD40 engagement, activates NF-kappa-B signaling pathway via phospholipase C and protein kinase C activation (By similarity). Isoform 2. Interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation. (Microbial infection) Acts as a receptor for adenovirus subgroup B.

Alternative names

CD86, CD28LG2, T-lymphocyte activation antigen CD86, Activation B7-2 antigen, B70, BU63, CTLA-4 counter-receptor B7.2, FUN-1

Recommended products

Mouse Monoclonal CD86 antibody. Carrier free. Suitable for Flow Cyt, IHC-Fr and reacts with Rat samples.

Key facts

Isotype
IgG1
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
OX48
Light chain type
kappa
Concentration
Loading...
Purification notes

Purified from TCS.

Notes

ab244583 is the carrier-free version of Anti-CD86 antibody [OX48] ab238468.

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CD86 also known as B7-2 is a protein involved in the regulation of the immune response. It has an approximate mass of 70 kDa and is expressed on antigen-presenting cells like dendritic cells monocytes and macrophages. Notably CD86 is present on macrophages including those in tissues such as skin and lymphoid organs. Expressed on these cells CD86 serves as a vital mediator in the co-stimulatory signals necessary for T cell activation and survival.

Biological function summary

CD86 plays a significant role in the immune system by providing secondary signals for T cell activation and differentiation. It is a part of the B7 protein family and forms a complex with CD28 and CTLA-4 on T cells. When CD86 binds to CD28 it sends positive co-stimulatory signals which promote T cell proliferation and cytokine production. On the other hand interaction with CTLA-4 transmits an inhibitory signal which reduces immune response. This dual interaction helps to balance immune activation and tolerance.

Pathways

CD86 takes part in important immune-related signaling pathways particularly the T cell receptor signaling pathway and the PI3K-Akt signaling pathway. Both pathways are fundamental for initiating immune responses. CD86's interaction with CD28 activates downstream signaling cascades including PI3K-Akt which is important for cell survival and growth. Additionally CD86 collaborates with other proteins such as CD80 another co-stimulatory molecule to amplify T cell activation within these pathways.

Associated diseases and disorders

CD86 is associated with autoimmune diseases and transplant rejection. In autoimmune diseases like rheumatoid arthritis the overexpression or dysregulation of CD86 can lead to excessive T cell activation causing immune system attacks on the body's own tissues. Similarly in transplant rejection CD86 may contribute by enhancing immune response against transplanted organs. The engagement between CD86 and CD28 is a critical factor in these conditions and therapies targeting this interaction are under exploration to mitigate the immune response.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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2 product images

  • Flow Cytometry - Anti-CD86 antibody [OX48] - BSA and Azide free (ab244583), expandable thumbnail

    Flow Cytometry - Anti-CD86 antibody [OX48] - BSA and Azide free (ab244583)

    This data was generating using the same clone in a different formulation (Anti-CD86 antibody [OX48] ab238468).

    Lewis rat splenocytes stained with Anti-CD86 antibody [OX48] ab238468 (right) or mouse IgG1κ (left). Lewis rat splenocytes were incubated for 30 min on ice in 10% rat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (Anti-CD86 antibody [OX48] ab238468) or mouse IgG1κ Isotype (Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control ab170190) (1x106 in 100μl at 5 μg/ml) for 30 min on ice.

    The secondary antibody Goat Anti-Mouse IgG H&L (Alexa Fluor ® 488, pre-adsorbed) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117) was used at 1/2000 dilution for 30 min at 4°C. The cells were simultaneously stained with CD45R PE antibody.

    Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on viable lymphocytes.

  • Immunohistochemistry (Frozen sections) - Anti-CD86 antibody [OX48] - BSA and Azide free (ab244583), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-CD86 antibody [OX48] - BSA and Azide free (ab244583)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (Anti-CD86 antibody [OX48] ab238468)

    IHC image of CD86 staining in a section of frozen normal rat spleen*.

    The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with Anti-CD86 antibody [OX48] ab238468 at 5μg/ml. The section was then incubated with Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed ab150119 (Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor®647, 1/1000)) (shown in red) for 1 hour at room temperature. The secondary-only control insert image is taken from an identical assay without primary antibody. The section was then mounted using Fluoromount®.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    For IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antibody concentrations and incubation times.

    *Tissue obtained from Charles River.

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Product protocols

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