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AB171221

Anti-CD86 antibody [PO3.1] - Low endotoxin, Azide free

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(1 Publication)

Rat Monoclonal CD86 antibody. Carrier free. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 1 publication.

View Alternative Names

CD86, CD28LG2, T-lymphocyte activation antigen CD86, Activation B7-2 antigen, B70, BU63, CTLA-4 counter-receptor B7.2, FUN-1

1 Images
Flow Cytometry - Anti-CD86 antibody [PO3.1] - Low endotoxin, Azide free (AB171221)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD86 antibody [PO3.1] - Low endotoxin, Azide free (AB171221)

Flow cytometric analysis of 2-day unstimulated (left panel) and 2-day LPS activated (right pabel) C57Bl/6 splenocytes labeling CD86 with 0.25 μg ab171221 (filled histogram) with Rat IgG2b K Isotype Control Functional Grade Purified (open histogram) followed by anti-Rat IgG FITC. Total viable cells were used for analysis.

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

PO3.1

Isotype

IgG2b

Light chain type

kappa

Carrier free

Yes

Reacts with

Mouse

Applications

Flow Cyt

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.25 µg for 10^5-10^8 Cells", "FlowCyt-species-notes": "<p>A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL.</p><p>ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.</p>" } } }
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Product details

Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD86 also known as B7-2 is a protein involved in the regulation of the immune response. It has an approximate mass of 70 kDa and is expressed on antigen-presenting cells like dendritic cells monocytes and macrophages. Notably CD86 is present on macrophages including those in tissues such as skin and lymphoid organs. Expressed on these cells CD86 serves as a vital mediator in the co-stimulatory signals necessary for T cell activation and survival.
Biological function summary

CD86 plays a significant role in the immune system by providing secondary signals for T cell activation and differentiation. It is a part of the B7 protein family and forms a complex with CD28 and CTLA-4 on T cells. When CD86 binds to CD28 it sends positive co-stimulatory signals which promote T cell proliferation and cytokine production. On the other hand interaction with CTLA-4 transmits an inhibitory signal which reduces immune response. This dual interaction helps to balance immune activation and tolerance.

Pathways

CD86 takes part in important immune-related signaling pathways particularly the T cell receptor signaling pathway and the PI3K-Akt signaling pathway. Both pathways are fundamental for initiating immune responses. CD86's interaction with CD28 activates downstream signaling cascades including PI3K-Akt which is important for cell survival and growth. Additionally CD86 collaborates with other proteins such as CD80 another co-stimulatory molecule to amplify T cell activation within these pathways.

CD86 is associated with autoimmune diseases and transplant rejection. In autoimmune diseases like rheumatoid arthritis the overexpression or dysregulation of CD86 can lead to excessive T cell activation causing immune system attacks on the body's own tissues. Similarly in transplant rejection CD86 may contribute by enhancing immune response against transplanted organs. The engagement between CD86 and CD28 is a critical factor in these conditions and therapies targeting this interaction are under exploration to mitigate the immune response.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4 (PubMed : 12196291). May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation (PubMed : 7527824). Also involved in the regulation of B cells function, plays a role in regulating the level of IgG(1) produced. Upon CD40 engagement, activates NF-kappa-B signaling pathway via phospholipase C and protein kinase C activation (By similarity).. Isoform 2. Interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.. (Microbial infection) Acts as a receptor for adenovirus subgroup B.
See full target information CD86
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of neurovirology 25:384-396 PubMed30758811

2019

Osteopontin counters human immunodeficiency virus type 1-induced impairment of neurite growth through mammalian target of rapamycin and beta-integrin signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Mathilde Calvez,George Hseeh,Simon Benzer,Amanda M Brown
View all publications
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