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AB270719

Anti-CD86 antibody [SPM600]

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(8 Publications)

Mouse Monoclonal CD86 antibody. Suitable for ICC/IF, IHC-P and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CD86.

View Alternative Names

CD86, CD28LG2, T-lymphocyte activation antigen CD86, Activation B7-2 antigen, B70, BU63, CTLA-4 counter-receptor B7.2, FUN-1

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD86 antibody [SPM600] (AB270719)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD86 antibody [SPM600] (AB270719)

Formalin-fixed, paraffin-embedded human melanoma tissue stained for CD86 using ab270719 at 4 μg/ml in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-CD86 antibody [SPM600] (AB270719)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CD86 antibody [SPM600] (AB270719)

Immunofluorescence analysis of Ramos cells labeling CD86 (Green) using ab270719 at 2 μg/ml, followed by Goat anti-Mouse IgG-CF488. The nuclear counterstain is Reddot (Red).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

SPM600

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human CD86.

P42081

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-2 µg/mL", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2-4 µg/mL", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Purification notes
Purified from bioreactor concentrate.
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD86 also known as B7-2 is a protein involved in the regulation of the immune response. It has an approximate mass of 70 kDa and is expressed on antigen-presenting cells like dendritic cells monocytes and macrophages. Notably CD86 is present on macrophages including those in tissues such as skin and lymphoid organs. Expressed on these cells CD86 serves as a vital mediator in the co-stimulatory signals necessary for T cell activation and survival.
Biological function summary

CD86 plays a significant role in the immune system by providing secondary signals for T cell activation and differentiation. It is a part of the B7 protein family and forms a complex with CD28 and CTLA-4 on T cells. When CD86 binds to CD28 it sends positive co-stimulatory signals which promote T cell proliferation and cytokine production. On the other hand interaction with CTLA-4 transmits an inhibitory signal which reduces immune response. This dual interaction helps to balance immune activation and tolerance.

Pathways

CD86 takes part in important immune-related signaling pathways particularly the T cell receptor signaling pathway and the PI3K-Akt signaling pathway. Both pathways are fundamental for initiating immune responses. CD86's interaction with CD28 activates downstream signaling cascades including PI3K-Akt which is important for cell survival and growth. Additionally CD86 collaborates with other proteins such as CD80 another co-stimulatory molecule to amplify T cell activation within these pathways.

CD86 is associated with autoimmune diseases and transplant rejection. In autoimmune diseases like rheumatoid arthritis the overexpression or dysregulation of CD86 can lead to excessive T cell activation causing immune system attacks on the body's own tissues. Similarly in transplant rejection CD86 may contribute by enhancing immune response against transplanted organs. The engagement between CD86 and CD28 is a critical factor in these conditions and therapies targeting this interaction are under exploration to mitigate the immune response.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4 (PubMed : 12196291). May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation (PubMed : 7527824). Also involved in the regulation of B cells function, plays a role in regulating the level of IgG(1) produced. Upon CD40 engagement, activates NF-kappa-B signaling pathway via phospholipase C and protein kinase C activation (By similarity).. Isoform 2. Interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.. (Microbial infection) Acts as a receptor for adenovirus subgroup B.
See full target information CD86
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

International journal of chronic obstructive pulmonary disease 20:551-564 PubMed40078929

2025

PGAM5 Modulates Macrophage Polarization, Aggravating Inflammation in COPD via the NF-κB Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Zheng,Yujie Wang,Jia Li,Shaomao Zheng,Lipeng Zhang,Qiaoyu Li,Fayu Ling,Qiuli Nie,Qiong Feng,Jing Wang,Chengji Jin

Journal of nanobiotechnology 23:175 PubMed40050923

2025

Regulating macrophage phenotypes with IL4I1-mimetic nanoparticles in IDD treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaying Luo,Guoxin Jin,Shaoqian Cui,Huan Wang,Qi Liu

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2413607 PubMed39755930

2025

Hydrogen Sulfide Promotes TAM-M1 Polarization through Activating IRE-1α Pathway via GRP78 S-Sulfhydrylation to against Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Mingyi Ju,Weiwei Tong,Jia Bi,Xianxin Zeng,Aoshuang Qi,Mingli Sun,Jian Wen,Lin Zhao,Minjie Wei

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2405924 PubMed39269428

2024

Nanocomposite Hydrogel for Real-Time Wound Status Monitoring and Comprehensive Treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Huan Lei,Xueqing Yu,Daidi Fan

Frontiers in immunology 13:1095879 PubMed36713449

2023

Placental macrophages present distinct polarization pattern and effector functions depending on clinical onset of preeclampsia.

Applications

Unspecified application

Species

Unspecified reactive species

Monika Horvat Mercnik,Carolin Schliefsteiner,Herbert Fluhr,Christian Wadsack

The Laryngoscope 133:2346-2356 PubMed36633350

2023

Myeloid Phenotypes in Tracheostomy-Associated Granulation Tissue.

Applications

Unspecified application

Species

Unspecified reactive species

Alexandra J Berges,Rafael Ospino,Ioan A Lina,Samuel Collins,Yee Chan-Li,Alexander Gelbard,Alexander T Hillel,Kevin M Motz

Journal of experimental & clinical cancer research : CR 41:174 PubMed35562774

2022

A positive feedback loop between gastric cancer cells and tumor-associated macrophage induces malignancy progression.

Applications

Unspecified application

Species

Unspecified reactive species

Haiyan Piao,Lingfeng Fu,Yuxin Wang,Yang Liu,Yue Wang,Xiangyu Meng,Dong Yang,Xiang Xiao,Jun Zhang

Cancer medicine 9:9554-9570 PubMed33058542

2020

Elevated expression of FREM1 in breast cancer indicates favorable prognosis and high-level immune infiltration status.

Applications

Unspecified application

Species

Unspecified reactive species

Han-Ning Li,Xing-Rui Li,Zheng-Tao Lv,Miao-Miao Cai,Ge Wang,Zhi-Fang Yang
View all publications
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