AB124717

Anti-CD89 antibody [EPR4622(2)]

RabMAb
Recombinant
What is this?

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(5 Publications)

Rabbit Recombinant Monoclonal CD89 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 5 publications.

View Alternative Names

CD89, FCAR, Immunoglobulin alpha Fc receptor, IgA Fc receptor

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD89 antibody [EPR4622(2)] (AB124717)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD89 antibody [EPR4622(2)] (AB124717)

ab124717 at 1/100 staining CD89 in paraffin embedded Human tonsil tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Lab

Immunoprecipitation - Anti-CD89 antibody [EPR4622(2)] (AB124717)

CD89 was immunoprecipitated from 0.35 mg U-937 (Human histiocytic lymphoma monocyte) whole cell lysate 10 μg with ab124717 at 1/50 dilution (2μg). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : U-937 (Human histiocytic lymphoma monocyte) whole cell lysate 10 μg

Lane 2 : ab124717 IP in U-937 whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab124717 in U-937 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-CD89 antibody [EPR4622(2)] (ab124717)

Predicted band size: 32 kDa

Observed band size: 70 kDa

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Western blot - Anti-CD89 antibody [EPR4622(2)] (AB124717)

All lanes:

Western blot - Anti-CD89 antibody [EPR4622(2)] (ab124717) at 1/1000 dilution

Lane 1:

U937 cell lysates at 10 µg

Lane 2:

K562 cell lysates at 10 µg

Lane 3:

Jurkat cell lysates at 10 µg

Lane 4:

HL-60 cell lysates at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 32 kDa

false

Carrier free

Anti-CD89 antibody [EPR4622(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4622(2)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD89 also known as the Fc alpha receptor (FcαR) is a protein receptor with a molecular mass of approximately 55 to 75 kDa. It is encoded by the FCAR gene and predominantly expressed on myeloid cells such as neutrophils eosinophils monocytes and some macrophages. CD89 acts as an important component of the immune system by binding to the Fc region of immunoglobulin A (IgA) facilitating various immune responses. This receptor mediates diverse immune functions by activating cellular responses upon IgA ligand binding.

Biological function summary

CD89 plays an important role in regulating immune surveillance and response. It associates with the gamma chain of the Fc receptor complex which is necessary for its signaling ability. The binding of IgA to CD89 triggers various processes including phagocytosis antibody-dependent cellular cytotoxicity (ADCC) and the release of pro-inflammatory cytokines. These processes are fundamental for host defense against pathogens particularly those entering through mucosal surfaces where IgA is typically abundant.

Pathways

CD89 is part of the immune effector pathway and participates actively in the immunoregulatory signaling cascade. One significant pathway is the IgA-mediated immune response pathway where CD89 cooperates with other Fc receptors and integrins to clear infections. Additionally its interaction with the complement system enhances opsonization and pathogen elimination. Related proteins in these pathways include other Fc receptors like FcγR which share similar signaling mechanisms and immune functions.

CD89 is associated with conditions involving dysregulated immune responses or infections. For example it is implicated in IgA nephropathy where abnormal IgA deposits in the kidneys may trigger inflammation through CD89-mediated pathways. Furthermore alterations in CD89 expression or function have been studied in the context of inflammatory bowel disease (IBD) illustrating the receptor's role in aberrant immune responses. The target’s interaction with proteins such as IgA and complement C3 provides insight into its involvement in these pathological conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Binds to the Fc region of immunoglobulins alpha. Mediates several functions including cytokine production.

See full target information FCAR

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:3995 PubMed40301356

2025

Enrichment of human IgA-coated bacterial vesicles in ulcerative colitis as a driver of inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Himadri B Thapa,Christina A Passegger,Dominik Fleischhacker,Paul Kohl,Yi-Chi Chen,Ratchara Kalawong,Carmen Tam-Amersdorfer,Michael R Gerstorfer,Jana Strahlhofer,Kristina Schild-Prüfert,Ellen L Zechner,Andreas Blesl,Lukas Binder,Georg A Busslinger,Leo Eberl,Gregor Gorkiewicz,Herbert Strobl,Christoph Högenauer,Stefan Schild

Nature communications 15:7784 PubMed39237503

2024

Unraveling the spatial organization and development of human thymocytes through integration of spatial transcriptomics and single-cell multi-omics profiling.

Applications

Unspecified application

Species

Unspecified reactive species

Yanchuan Li,Huamei Li,Cheng Peng,Ge Meng,Yijun Lu,Honglin Liu,Li Cui,Huan Zhou,Zhu Xu,Lingyun Sun,Lihong Liu,Qing Xiong,Beicheng Sun,Shiping Jiao

Bio-protocol 12: PubMed35937933

2022

Serological Measurement of Poly-IgA Immune Complex Levels in IgA Nephropathy and IgA Vasculitis.

Applications

Unspecified application

Species

Unspecified reactive species

Xue Zhang,Jicheng Lv,Pan Liu,Xinfang Xie,Xinyan Li,Hong Zhang,Jing Jin

Biomedicines 10: PubMed35625932

2022

Clinical, Histopathologic, and Immunohistochemical Features of Patients with IgG/IgA Pemphigus.

Applications

Unspecified application

Species

Unspecified reactive species

Yung-Tsu Cho,Ko-Ting Fu,Kai-Lung Chen,Yih-Leong Chang,Chia-Yu Chu

Experimental and therapeutic medicine 20:2752-2764 PubMed32765770

2020

Bone marrow stromal cells-derived exosomes target DAB2IP to induce microglial cell autophagy, a new strategy for neural stem cell transplantation in brain injury.

Applications

Unspecified application

Species

Unspecified reactive species

Feng-Ying Yuan,Ming-Xing Zhang,Yi-Hua Shi,Mei-Hui Li,Jia-Yuan Ou,Wen-Fang Bai,Ming-Sheng Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD89 antibody [EPR4622(2)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD89 antibody [EPR4622(2)] (AB124717)

Immunoprecipitation - Anti-CD89 antibody [EPR4622(2)] (AB124717)

Western blot - Anti-CD89 antibody [EPR4622(2)] (AB124717)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise