AB247978

Anti-CD89 antibody [EPR4622(2)] - BSA and Azide free

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal CD89 antibody. Carrier free. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

CD89, FCAR, Immunoglobulin alpha Fc receptor, IgA Fc receptor

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD89 antibody [EPR4622(2)] - BSA and Azide free (AB247978)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD89 antibody [EPR4622(2)] - BSA and Azide free (AB247978)

This data was developed using ab124717, the same antibody clone in a different buffer formulation.

ab124717, at 1/100, staining CD89 in paraffin embedded Human tonsil tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Lab

Immunoprecipitation - Anti-CD89 antibody [EPR4622(2)] - BSA and Azide free (AB247978)

This data was developed using ab124717, the same antibody clone in a different buffer formulation.

CD89 was immunoprecipitated from 0.35 mg U-937 (Human histiocytic lymphoma monocyte) whole cell lysate 10 μg with ab124717 at 1/50 dilution (2μg). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : U-937 (Human histiocytic lymphoma monocyte) whole cell lysate 10 μg

Lane 2 : ab124717 IP in U-937 whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab124717 in U-937 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-CD89 antibody [EPR4622(2)] (<a href='/en-us/products/primary-antibodies/cd89-antibody-epr46222-ab124717'>ab124717</a>)

Predicted band size: 32 kDa

Observed band size: 70 kDa

false

Unknown

Western blot - Anti-CD89 antibody [EPR4622(2)] - BSA and Azide free (AB247978)

This data was developed using ab124717, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-CD89 antibody [EPR4622(2)] (<a href='/en-us/products/primary-antibodies/cd89-antibody-epr46222-ab124717'>ab124717</a>) at 1/1000 dilution

Lane 1:

U937 cell lysates at 10 µg

Lane 2:

K562 cell lysates at 10 µg

Lane 3:

Jurkat cell lysates at 10 µg

Lane 4:

HL-60 cell lysates at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 32 kDa

false

Unconjugated

Anti-CD89 antibody [EPR4622(2)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4622(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

ab247978 is the carrier-free version of ab124717.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD89 also known as the Fc alpha receptor (FcαR) is a protein receptor with a molecular mass of approximately 55 to 75 kDa. It is encoded by the FCAR gene and predominantly expressed on myeloid cells such as neutrophils eosinophils monocytes and some macrophages. CD89 acts as an important component of the immune system by binding to the Fc region of immunoglobulin A (IgA) facilitating various immune responses. This receptor mediates diverse immune functions by activating cellular responses upon IgA ligand binding.

Biological function summary

CD89 plays an important role in regulating immune surveillance and response. It associates with the gamma chain of the Fc receptor complex which is necessary for its signaling ability. The binding of IgA to CD89 triggers various processes including phagocytosis antibody-dependent cellular cytotoxicity (ADCC) and the release of pro-inflammatory cytokines. These processes are fundamental for host defense against pathogens particularly those entering through mucosal surfaces where IgA is typically abundant.

Pathways

CD89 is part of the immune effector pathway and participates actively in the immunoregulatory signaling cascade. One significant pathway is the IgA-mediated immune response pathway where CD89 cooperates with other Fc receptors and integrins to clear infections. Additionally its interaction with the complement system enhances opsonization and pathogen elimination. Related proteins in these pathways include other Fc receptors like FcγR which share similar signaling mechanisms and immune functions.

CD89 is associated with conditions involving dysregulated immune responses or infections. For example it is implicated in IgA nephropathy where abnormal IgA deposits in the kidneys may trigger inflammation through CD89-mediated pathways. Furthermore alterations in CD89 expression or function have been studied in the context of inflammatory bowel disease (IBD) illustrating the receptor's role in aberrant immune responses. The target’s interaction with proteins such as IgA and complement C3 provides insight into its involvement in these pathological conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Binds to the Fc region of immunoglobulins alpha. Mediates several functions including cytokine production.

See full target information FCAR

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Clinics (Sao Paulo, Brazil) 77:100019 PubMed35397366

2022

IRF4 suppresses osteogenic differentiation of BM-MSCs by transcriptionally activating miR-636/DOCK9 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xuepu Zhang,Yue Zhang,Limin Yang,Yuexin Wu,Xiaohu Ma,Gang Tong,Zhaoliang Ban,Haosen Zhao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD89 antibody [EPR4622(2)] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD89 antibody [EPR4622(2)] - BSA and Azide free (AB247978)

Immunoprecipitation - Anti-CD89 antibody [EPR4622(2)] - BSA and Azide free (AB247978)

Western blot - Anti-CD89 antibody [EPR4622(2)] - BSA and Azide free (AB247978)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise