Anti-CD9 antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody (AB223052)

Paraffin embedded sections of human colon cancer tissue were stained for CD9 with ab223052 at 1/100 dilution in immunohistochemical analysis.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody (AB223052)

Paraffin embedded sections of human placenta tissue were stained for CD9 with ab223052 at 1/100 dilution in immunohistochemical analysis.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody (AB223052)

Left : IHC image of ab223052 diluted at 1 : 100 and staining in paraffin-embedded human tonsils tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Right : Negative Control

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody (AB223052)

Left : IHC image of ab223052 diluted at 1 : 100 and staining in paraffin-embedded human bladder tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Right : Negative Control

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody (AB223052)

Left : IHC image of ab223052 diluted at 1 : 100 and staining in paraffin-embedded human Prostate gland Cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Right : negative control

• WB

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Western blot - Anti-CD9 antibody (AB223052)

All lanes:

Western blot - Anti-CD9 antibody (ab223052) at 1/1000 dilution

Lane 1:

HepG2 whole cell lysate at 20 µg

Lane 2:

HepG2 whole cell lysate at 40 µg

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 25 kDa

Observed band size: 25 kDa

false

• WB

Supplier Data

Western blot - Anti-CD9 antibody (AB223052)

All lanes:

Western blot - Anti-CD9 antibody (ab223052) at 1/1000 dilution

Lane 1:

Hela whole cell lysate at 20 µg

Lane 2:

HepG2 whole cell lysate at 20 µg

Lane 3:

LO2 whole cell lysate at 20 µg

Lane 4:

A549 whole cell lysate at 20 µg

Lane 5:

U87 whole cell lysate at 20 µg

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 25 kDa

Observed band size: 25 kDa

false

• WB

Supplier Data

Western blot - Anti-CD9 antibody (AB223052)

All lanes:

Western blot - Anti-CD9 antibody (ab223052) at 1/500 dilution

Lane 1:

Rat Liver tissue lysate at 20 µg

Lane 2:

Rat Lung tissue lysate at 20 µg

Lane 3:

Mouse Liver tissue lysate at 20 µg

Lane 4:

Mouse Lung tissue lysate at 20 µg

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 25 kDa

Observed band size: 25 kDa

false

• WB

CiteAb

Western blot - Anti-CD9 antibody (AB223052)

CD9 western blot using anti-CD9 antibody ab223052. Publication image and figure legend from Gao, K., Jin, J., et al., 2019, Front Immunol, PubMed 31354717.

ab223052 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab223052 please see the product overview.

Characterization of exosomes derived from septic mouse serum. (A) Electron microscope (EM) analysis of exosomes. The circular particles representing exosomes are indicated by arrows. The scale bar represents a length of 200 nm. (B) Exosomes containing 20 μg of proteins were used to detect exosome-specific proteins (CD9, CD63, and CD81) by Western blot. (C) The size distribution of exosomes was determined by nanoparticle tracking analysis (NTA). (D) Dynamic change in exosome protein concentrations. The exosomes were separated from 200 μl of serum harvested from septic mice at different time points for protein quantitation. (E) The sequential detection of exosomes derived from septic mouse serum. The particle sizes of exosomes were analyzed by the NTA technique. Data are shown as the mean ± SD from three independent experiments (n = 3) and analyzed by one-way ANOVA. *p < 0.05, compared with the 0 h as the control.

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