Anti-CD9 rabbit polyclonal antibody that is used to detect CD9 in Western blot, IHC. Suitable for Human, Mouse samples.
- Cited in over 50 publications
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- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 38:e233632024Single cell RNA sequencing reveals emergent notochord-derived cell subpopulations in the postnatal nucleus pulposus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChenghao Zhang,Leilei Zhong,Yian Khai Lau,Meilun Wu,Lutian Yao,Thomas P Schaer,Robert L Mauck,Neil R Malhotra,Ling Qin,Lachlan J SmithPubMed 38085183
- Cell death & disease 14:7082023The microprotein encoded by exosomal lncAKR1C2 promotes gastric cancer lymph node metastasis by regulating fatty acid metabolism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKe-Gan Zhu,Jiayu Yang,Yuehong Zhu,Qihang Zhu,Wen Pan,Siyu Deng,Yi He,Duo Zuo,Peiyun Wang,Yueting Han,Hai-Yang ZhangPubMed 37903800
- Communications biology 6:8852023Selective enrichment of plasma cell-free messenger RNA in cancer-associated extracellular vesicles.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHyun Ji Kim,Matthew J Rames,Florian Goncalves,C Ward Kirschbaum,Breeshey Roskams-Hieter,Elias Spiliotopoulos,Josephine Briand,Aaron Doe,Joseph Estabrook,Josiah T Wagner,Emek Demir,Gordon Mills,Thuy T M NgoPubMed 37644220
- PloS one 18:e02842582023Matrix vesicles promote bone repair after a femoral bone defect in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuya Mizukami,Naoyuki Kawao,Yoshimasa Takafuji,Takashi Ohira,Kiyotaka Okada,Jun-Ichiro Jo,Yasuhiko Tabata,Hiroshi KajiPubMed 37027385
- Biology direct 18:62023ADSC-derived exosomes attenuate myocardial infarction injury by promoting miR-205-mediated cardiac angiogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTingting Wang,Tao Li,Xiaolin Niu,Lang Hu,Jin Cheng,Dong Guo,He Ren,Ran Zhao,Zhaole Ji,Pengyun Liu,Yan Li,Yanjie GuoPubMed 36849959
- Aging and disease 14:229-2442023Long RNA Profiles of Human Brain Extracellular Vesicles Provide New Insights into the Pathogenesis of Alzheimer's Disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDan Luo,Haotian Liu,Hanyou Liu,Wei Wu,Hanyang Zhu,Wei Ge,Chao MaPubMed 36818567
- Journal of neuroinflammation 20:202023Plasma derived extracellular vesicle biomarkers of microglia activation in an experimental stroke model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesA D Roseborough,S J Myers,R Khazaee,Y Zhu,L Zhao,E Iorio,F M Elahi,S H Pasternak,S N WhiteheadPubMed 36721258
- Histology and histopathology 38:1295-13062023Hsa_circ_0072765 knockdown inhibits proliferation, activation and migration in transforming growth factor-beta (TGF-β)-induced hepatic stellate cells (HSCs) by the miR-197-3p/TRPV3 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan Jin,Xueyan Guo,Rong Zhang,Chunying YanPubMed 36714978
- EMBO reports 24:e555322023T cell-dependent bispecific antibodies alter organ-specific endothelial cell-T cell interaction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPatricia Himmels,Thi Thu Thao Nguyen,Maresa Caunt Mitzner,Alfonso Arrazate,Stacey Yeung,Jeremy Burton,Robyn Clark,Klara Totpal,Raj Jesudason,Angela Yang,Margaret Solon,Jeffrey Eastham,Zora Modrusan,Joshua D Webster,Amy A Lo,Robert Piskol,Weilan YePubMed 36621885
- International journal of molecular sciences 23:2022Cancer-Specific miRNAs Extracted from Tissue-Exudative Extracellular Vesicles in Ovarian Clear Cell Carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHiroshi Maruoka,Tomohito Tanaka,Hikaru Murakami,Hiromitsu Tsuchihashi,Akihiko Toji,Misa Nunode,Atsushi Daimon,Shunsuke Miyamoto,Ruri Nishie,Shoko Ueda,Sousuke Hashida,Shinichi Terada,Hiromi Konishi,Yuhei Kogata,Kohei Taniguchi,Kazumasa Komura,Masahide OhmichiPubMed 36555361
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human CD9 aa 100-200. The exact immunogen used to generate this antibody is proprietary information. Database link P21926
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Expected |
| Rat | Tested | Expected |
| African green monkey | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/500 | Notes - |
Species Mouse | Dilution info 1/500 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species African green monkey | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/500.00000 | Notes We do not guarantee IHC-P for mouse and rat. We recommend ab307085 as an alternative for IHC-P. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species African green monkey | Dilution info - | Notes - |
Associated Products
Select an associated product type
13 products for Alternative Product
Target data
Function
Integral membrane protein associated with integrins, which regulates different processes, such as sperm-egg fusion, platelet activation and aggregation, and cell adhesion (PubMed:14575715, PubMed:18541721, PubMed:8478605). Present at the cell surface of oocytes and plays a key role in sperm-egg fusion, possibly by organizing multiprotein complexes and the morphology of the membrane required for the fusion (By similarity). In myoblasts, associates with CD81 and PTGFRN and inhibits myotube fusion during muscle regeneration (By similarity). In macrophages, associates with CD81 and beta-1 and beta-2 integrins, and prevents macrophage fusion into multinucleated giant cells specialized in ingesting complement-opsonized large particles (PubMed:12796480). Also prevents the fusion between mononuclear cell progenitors into osteoclasts in charge of bone resorption (By similarity). Acts as a receptor for PSG17 (By similarity). Involved in platelet activation and aggregation (PubMed:18541721). Regulates paranodal junction formation (By similarity). Involved in cell adhesion, cell motility and tumor metastasis (PubMed:7511626, PubMed:8478605).
Alternative names
CD9, MIC3, TSPAN29, GIG2, CD9 antigen, 5H9 antigen, Cell growth-inhibiting gene 2 protein, Leukocyte antigen MIC3, Motility-related protein, Tetraspanin-29, p24, MRP-1, Tspan-29
Recommended products
Anti-CD9 rabbit polyclonal antibody that is used to detect CD9 in Western blot, IHC. Suitable for Human, Mouse samples.
- Cited in over 50 publications
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human CD9 aa 100-200. The exact immunogen used to generate this antibody is proprietary information. Database link P21926
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
>95% pure
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Product Specifications
Anti-CD9 antibody (ab223052) is a rabbit polyclonal antibody and is validated for use in IHC-P, WB in human, mouse samples.
Anti-CD9 antibody (ab223052) specifically detects CD9 (UniProt ID: P21926; Molecular weight: 25kDa) and is sold in 50 µL selling sizes.
Quality and Validation
Abcam's high quality validation processes ensure Anti-CD9 antibody (ab223052) has high sensitivity and specificity.
Anti-CD9 antibody (ab223052) has been cited over 60 times in peer reviewed journals and is trusted by the scientific community.
Target Information
CD9 also known as p24 is a member of the tetraspanin family, which includes proteins involved in various cellular processes. CD9 (p24) plays a significant role in cancer progression and metastasis by influencing cell adhesion, migration, and signaling. CD9 (p24) is found on the surface of cancer cells and within exosomes, impacting cancer progression and therapy resistance. CD9's role in cancer is complex, as it can both suppress and promote tumor growth depending on the context. The protein's interactions within tetraspanin-enriched microdomains (TEMs) are crucial for its function.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD9 also known as motility-related protein-1 (MRP-1) is a transmembrane glycoprotein with a molecular weight of approximately 24 kDa. This protein is part of the tetraspanin family and shows widespread expression throughout different tissues including hematopoietic and non-hematopoietic cells. CD9 participates in numerous cellular processes by interacting with integrins and other cell surface receptors facilitating the organization of molecular complexes within the cell membrane.
- Biological function summary
The CD9 protein is integral to cell adhesion migration and fusion. It forms complexes with other tetraspanins and proteins like EWI-2 and EWI-F contributing to cellular signaling and membrane compartmentalization. Additionally CD9 plays an important role in the formation and secretion of exosomes tiny vesicles emitted by cells that mediate cell-to-cell communication. These exosomes can be analyzed using tools like exosome assays and detection kits that target CD9 proteins.
- Pathways
CD9 is involved in the regulation of immune response and cell morphology. It participates in pathways such as the integrin signaling pathway and the phosphatidylinositol 3-kinase (PI3K) signaling pathway. CD9 modulates these interactions by associating with proteins such as integrins and other members of the tetraspanin family influencing cellular movement and proliferation.
- Associated diseases and disorders
CD9 has connections to various conditions including cancer and infectious diseases. In cancer CD9 can influence tumor progression and metastasis while its altered expression levels have been associated with different cancer types. CD9 interacts with proteins such as integrins and CD81 in these contexts affecting cellular adhesion and migration mechanisms. In infectious diseases CD9 is involved in viral entry processes as some viruses utilize CD9 and its associated tetraspanins for entry into host cells.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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9 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody (ab223052)
Paraffin embedded sections of human colon cancer tissue were stained for CD9 with ab223052 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody (ab223052)
Paraffin embedded sections of human placenta tissue were stained for CD9 with ab223052 at 1/100 dilution in immunohistochemical analysis.
Western blot - Anti-CD9 antibody (ab223052)
All lanes: Western blot - Anti-CD9 antibody (ab223052) at 1/1000 dilution
Lane 1: HepG2 whole cell lysate at 20 µg
Lane 2: HepG2 whole cell lysate at 40 µg
SecondaryAll lanes: Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDa
Western blot - Anti-CD9 antibody (ab223052)
All lanes: Western blot - Anti-CD9 antibody (ab223052) at 1/1000 dilution
Lane 1: Hela whole cell lysate at 20 µg
Lane 2: HepG2 whole cell lysate at 20 µg
Lane 3: LO2 whole cell lysate at 20 µg
Lane 4: A549 whole cell lysate at 20 µg
Lane 5: U87 whole cell lysate at 20 µg
SecondaryAll lanes: Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody (ab223052)
Left: IHC image of ab223052 diluted at 1:100 and staining in paraffin-embedded human tonsils tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Right: Negative Control
Western blot - Anti-CD9 antibody (ab223052)
All lanes: Western blot - Anti-CD9 antibody (ab223052) at 1/500 dilution
Lane 1: Rat Liver tissue lysate at 20 µg
Lane 2: Rat Lung tissue lysate at 20 µg
Lane 3: Mouse Liver tissue lysate at 20 µg
Lane 4: Mouse Lung tissue lysate at 20 µg
SecondaryAll lanes: Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody (ab223052)
Left: IHC image of ab223052 diluted at 1:100 and staining in paraffin-embedded human bladder tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Right: Negative Control
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody (ab223052)
Left: IHC image of ab223052 diluted at 1:100 and staining in paraffin-embedded human Prostate gland Cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Right: negative control
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-CD9 antibody (ab223052)
CD9 western blot using anti-CD9 antibody ab223052. Publication image and figure legend from Gao, K., Jin, J., et al., 2019, Front Immunol, PubMed 31354717.
ab223052 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab223052 please see the product overview.
Characterization of exosomes derived from septic mouse serum. (A) Electron microscope (EM) analysis of exosomes. The circular particles representing exosomes are indicated by arrows. The scale bar represents a length of 200 nm. (B) Exosomes containing 20 μg of proteins were used to detect exosome-specific proteins (CD9, CD63, and CD81) by Western blot. (C) The size distribution of exosomes was determined by nanoparticle tracking analysis (NTA). (D) Dynamic change in exosome protein concentrations. The exosomes were separated from 200 μl of serum harvested from septic mice at different time points for protein quantitation. (E) The sequential detection of exosomes derived from septic mouse serum. The particle sizes of exosomes were analyzed by the NTA technique. Data are shown as the mean ± SD from three independent experiments (n = 3) and analyzed by one-way ANOVA. *P < 0.05, compared with the 0 h as the control.
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