AB82390

Anti-CD9 antibody [EM-04]

Name: Anti-CD9 antibody [EM-04] (ab82390) | Abcam
Brand: Abcam Limited
SKU: AB82390
Rating: 3 (1 reviews)

(1 Review)

(9 Publications)

Rat Monoclonal CD9 antibody. Suitable for WB and reacts with Mouse samples. Cited in 9 publications. Immunogen corresponding to Cell preparation containing CD9 antigen protein.

View Alternative Names

CD9, CD9 antigen, Cd9

1 Images

Unknown

Western blot - Anti-CD9 antibody [EM-04] (AB82390)

All lanes:

Western blot - Anti-CD9 antibody [EM-04] (ab82390) at 1 µg/mL

Lane 1:

lysate from 1X10^6 mouse bone marrow-derived mast cell (BMMC)

Lane 2:

lysate from 0.5X10^6 mouse bone marrow-derived mast cell (BMMC)

Lane 3:

lysate from 0.25X10^6 mouse bone marrow-derived mast cell (BMMC)

Lane 4:

lysate from 0.125X10^6 mouse bone marrow-derived mast cell (BMMC)

Predicted band size: 25 kDa

Observed band size: 24 kDa

false

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

EM-04

Isotype

IgG1

Carrier free

Reacts with

Mouse

Applications

applications

Immunogen

Cell preparation containing CD9 antigen protein. The exact immunogen used to generate this antibody is proprietary information.

P40240

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified by protein-A affinity chromatography

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD9 also known as motility-related protein-1 (MRP-1) is a transmembrane glycoprotein with a molecular weight of approximately 24 kDa. This protein is part of the tetraspanin family and shows widespread expression throughout different tissues including hematopoietic and non-hematopoietic cells. CD9 participates in numerous cellular processes by interacting with integrins and other cell surface receptors facilitating the organization of molecular complexes within the cell membrane.

Biological function summary

The CD9 protein is integral to cell adhesion migration and fusion. It forms complexes with other tetraspanins and proteins like EWI-2 and EWI-F contributing to cellular signaling and membrane compartmentalization. Additionally CD9 plays an important role in the formation and secretion of exosomes tiny vesicles emitted by cells that mediate cell-to-cell communication. These exosomes can be analyzed using tools like exosome assays and detection kits that target CD9 proteins.

Pathways

CD9 is involved in the regulation of immune response and cell morphology. It participates in pathways such as the integrin signaling pathway and the phosphatidylinositol 3-kinase (PI3K) signaling pathway. CD9 modulates these interactions by associating with proteins such as integrins and other members of the tetraspanin family influencing cellular movement and proliferation.

CD9 has connections to various conditions including cancer and infectious diseases. In cancer CD9 can influence tumor progression and metastasis while its altered expression levels have been associated with different cancer types. CD9 interacts with proteins such as integrins and CD81 in these contexts affecting cellular adhesion and migration mechanisms. In infectious diseases CD9 is involved in viral entry processes as some viruses utilize CD9 and its associated tetraspanins for entry into host cells.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Integral membrane protein associated with integrins, which regulates different processes, such as sperm-egg fusion, platelet activation and aggregation, and cell adhesion (PubMed : 10634790, PubMed : 10634791, PubMed : 10700183, PubMed : 14715942). Present at the cell surface of oocytes and plays a key role in sperm-egg fusion, possibly by organizing multiprotein complexes and the morphology of the membrane required for the fusion (PubMed : 10634790, PubMed : 10634791, PubMed : 10700183, PubMed : 21690351). In myoblasts, associates with CD81 and PTGFRN and inhibits myotube fusion during muscle regeneration (PubMed : 23575678). In macrophages, associates with CD9 and beta-1 and beta-2 integrins, and prevents macrophage fusion into multinucleated giant cells specialized in ingesting complement-opsonized large particles. Also prevents the fusion between mononuclear cell progenitors into osteoclasts in charge of bone resorption (PubMed : 12796480). Acts as a receptor for PSG17 (PubMed : 11805154). Involved in platelet activation and aggregation (PubMed : 14715942). Regulates paranodal junction formation (PubMed : 14715942). Involved in cell adhesion, cell motility and tumor metastasis (By similarity). Also regulates integrin-dependent migration of macrophages, particularly relevant for inflammatory response in the lung (PubMed : 18662991).

See full target information CD9 antigen

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Journal of extracellular vesicles 14:e70091 PubMed40545975

2025

Extracellular Vesicles From Prostate Cancer-Corrupted Osteoclasts Drive a Chain Reaction of Inflammatory Osteolysis and Tumour Progression at the Bone Metastatic Site.

Applications

Unspecified application

Species

Unspecified reactive species

Takaaki Tamura,Tomofumi Yamamoto,Akiko Kogure,Yusuke Yoshioka,Yusuke Yamamoto,Shinichi Sakamoto,Tomohiko Ichikawa,Takahiro Ochiya

Cancer immunology, immunotherapy : CII 74:193 PubMed40343498

2025

Osteosarcoma cell-derived CCL2 facilitates lung metastasis via accumulation of tumor-associated macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Hiroya Kondo,Hiroshi Tazawa,Tomohiro Fujiwara,Aki Yoshida,Miho Kure,Koji Demiya,Nobuhiko Kanaya,Toshiaki Hata,Koji Uotani,Joe Hasei,Toshiyuki Kunisada,Shunsuke Kagawa,Yusuke Yoshioka,Toshifumi Ozaki,Toshiyoshi Fujiwara

Cellular and molecular life sciences : CMLS 82:165 PubMed40249512

2025

Involvement of miRNA-204 carried by the exosomes of macrophages in the AT2 receptor-mediated improvement of vascular calcification.

Applications

Unspecified application

Species

Unspecified reactive species

Hui-Yu Bai,Xiao-Rui Lv,Hai-Bo Gu,Hui Li,Bao-Shuai Shan

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 38:e23363 PubMed38085183

2024

Single cell RNA sequencing reveals emergent notochord-derived cell subpopulations in the postnatal nucleus pulposus.

Applications

Unspecified application

Species

Unspecified reactive species

Chenghao Zhang,Leilei Zhong,Yian Khai Lau,Meilun Wu,Lutian Yao,Thomas P Schaer,Robert L Mauck,Neil R Malhotra,Ling Qin,Lachlan J Smith

Frontiers in molecular neuroscience 16:1146329 PubMed37305554

2023

Muscle-derived stem cell exosomes with overexpressed miR-214 promote the regeneration and repair of rat sciatic nerve after crush injury to activate the JAK2/STAT3 pathway by targeting PTEN.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangyu Zeng,Wei Bian,Ziwen Liu,Jianming Li,Shuai Ren,Jian Zhang,Haoran Zhang,Bu Tegeleqi,Guanyi He,Mingyan Guan,Zewei Gao,Chi Huang,Jianyu Liu

Journal of extracellular vesicles 11:e12251 PubMed36043432

2022

In situ hybridization to detect DNA amplification in extracellular vesicles.

Applications

Unspecified application

Species

Unspecified reactive species

Lucia Casadei,Patricia Sarchet,Fernanda Costas C de Faria,Federica Calore,Giovanni Nigita,Sayumi Tahara,Luciano Cascione,Martin Wabitsch,Francis J Hornicek,Valerie Grignol,Carlo M Croce,Raphael E Pollock

Frontiers in immunology 9:555 PubMed29616043

2018

Lineage-Specific Analysis of Syk Function in Autoantibody-Induced Arthritis.

Applications

Unspecified application

Species

Mouse

Tamás Németh,Krisztina Futosi,Kata Szilveszter,Olivér Vilinovszki,Levente Kiss-Pápai,Attila Mócsai

Nature medicine 23:1176-1190 PubMed28920957

2017

KLF4-dependent perivascular cell plasticity mediates pre-metastatic niche formation and metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Meera Murgai,Wei Ju,Matthew Eason,Jessica Kline,Daniel W Beury,Sabina Kaczanowska,Markku M Miettinen,Michael Kruhlak,Haiyan Lei,Jack F Shern,Olga A Cherepanova,Gary K Owens,Rosandra N Kaplan

Molecular therapy : the journal of the American So 25:181-191 PubMed28129113

2017

Disruption of Circulating Extracellular Vesicles as a Novel Therapeutic Strategy against Cancer Metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Nao Nishida-Aoki,Naoomi Tominaga,Fumitaka Takeshita,Hikaru Sonoda,Yusuke Yoshioka,Takahiro Ochiya

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD9 antibody [EM-04]

Western blot - Anti-CD9 antibody [EM-04] (AB82390)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

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