Rat Monoclonal CD9 antibody. Suitable for WB and reacts with Mouse samples. Cited in 5 publications. Immunogen corresponding to Cell preparation containing CD9 antigen protein.
Images
![Western blot - Anti-CD9 antibody [EM-04] (AB82390), expandable thumbnail](https://content.abcam.com/products/images/cd9-antibody-em-04-ab82390--western-blot-img43155.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CD9 antibody [EM-04] (AB82390)")
Publications
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- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 38:e233632024Single cell RNA sequencing reveals emergent notochord-derived cell subpopulations in the postnatal nucleus pulposus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChenghao Zhang,Leilei Zhong,Yian Khai Lau,Meilun Wu,Lutian Yao,Thomas P Schaer,Robert L Mauck,Neil R Malhotra,Ling Qin,Lachlan J SmithPubMed 38085183
- Journal of extracellular vesicles 11:e122512022In situ hybridization to detect DNA amplification in extracellular vesicles.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLucia Casadei,Patricia Sarchet,Fernanda Costas C de Faria,Federica Calore,Giovanni Nigita,Sayumi Tahara,Luciano Cascione,Martin Wabitsch,Francis J Hornicek,Valerie Grignol,Carlo M Croce,Raphael E PollockPubMed 36043432
- Frontiers in immunology 9:5552018Lineage-Specific Analysis of Syk Function in Autoantibody-Induced Arthritis.
- Nature medicine 23:1176-11902017KLF4-dependent perivascular cell plasticity mediates pre-metastatic niche formation and metastasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMeera Murgai,Wei Ju,Matthew Eason,Jessica Kline,Daniel W Beury,Sabina Kaczanowska,Markku M Miettinen,Michael Kruhlak,Haiyan Lei,Jack F Shern,Olga A Cherepanova,Gary K Owens,Rosandra N KaplanPubMed 28920957
- Molecular therapy : the journal of the American So 25:181-1912017Disruption of Circulating Extracellular Vesicles as a Novel Therapeutic Strategy against Cancer Metastasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNao Nishida-Aoki et. al.PubMed 28129113
Key facts
- Isotype
- IgG1
- Host species
- Rat
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Cell preparation containing CD9 antigen protein. The exact immunogen used to generate this antibody is proprietary information. Database link P40240
Reactivity data
Select an application| WB | |
|---|---|
| Mouse | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
Associated Products
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Target data
Function
Integral membrane protein associated with integrins, which regulates different processes, such as sperm-egg fusion, platelet activation and aggregation, and cell adhesion (PubMed:10634790, PubMed:10634791, PubMed:10700183, PubMed:14715942). Present at the cell surface of oocytes and plays a key role in sperm-egg fusion, possibly by organizing multiprotein complexes and the morphology of the membrane required for the fusion (PubMed:10634790, PubMed:10634791, PubMed:10700183, PubMed:21690351). In myoblasts, associates with CD81 and PTGFRN and inhibits myotube fusion during muscle regeneration (PubMed:23575678). In macrophages, associates with CD9 and beta-1 and beta-2 integrins, and prevents macrophage fusion into multinucleated giant cells specialized in ingesting complement-opsonized large particles. Also prevents the fusion between mononuclear cell progenitors into osteoclasts in charge of bone resorption (PubMed:12796480). Acts as a receptor for PSG17 (PubMed:11805154). Involved in platelet activation and aggregation (PubMed:14715942). Regulates paranodal junction formation (PubMed:14715942). Involved in cell adhesion, cell motility and tumor metastasis (By similarity). Also regulates integrin-dependent migration of macrophages, particularly relevant for inflammatory response in the lung (PubMed:18662991).
Alternative names
CD9, CD9 antigen, Cd9
Recommended products
Rat Monoclonal CD9 antibody. Suitable for WB and reacts with Mouse samples. Cited in 5 publications. Immunogen corresponding to Cell preparation containing CD9 antigen protein.
Key facts
- Isotype
- IgG1
- Host species
- Rat
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Cell preparation containing CD9 antigen protein. The exact immunogen used to generate this antibody is proprietary information. Database link P40240
- Clone number
- EM-04
- Purification technique
- Affinity purification Protein G
- Concentration
- Purification notes
Purified by protein-A affinity chromatography
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD9 also known as motility-related protein-1 (MRP-1) is a transmembrane glycoprotein with a molecular weight of approximately 24 kDa. This protein is part of the tetraspanin family and shows widespread expression throughout different tissues including hematopoietic and non-hematopoietic cells. CD9 participates in numerous cellular processes by interacting with integrins and other cell surface receptors facilitating the organization of molecular complexes within the cell membrane.
- Biological function summary
The CD9 protein is integral to cell adhesion migration and fusion. It forms complexes with other tetraspanins and proteins like EWI-2 and EWI-F contributing to cellular signaling and membrane compartmentalization. Additionally CD9 plays an important role in the formation and secretion of exosomes tiny vesicles emitted by cells that mediate cell-to-cell communication. These exosomes can be analyzed using tools like exosome assays and detection kits that target CD9 proteins.
- Pathways
CD9 is involved in the regulation of immune response and cell morphology. It participates in pathways such as the integrin signaling pathway and the phosphatidylinositol 3-kinase (PI3K) signaling pathway. CD9 modulates these interactions by associating with proteins such as integrins and other members of the tetraspanin family influencing cellular movement and proliferation.
- Associated diseases and disorders
CD9 has connections to various conditions including cancer and infectious diseases. In cancer CD9 can influence tumor progression and metastasis while its altered expression levels have been associated with different cancer types. CD9 interacts with proteins such as integrins and CD81 in these contexts affecting cellular adhesion and migration mechanisms. In infectious diseases CD9 is involved in viral entry processes as some viruses utilize CD9 and its associated tetraspanins for entry into host cells.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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1 product image
![Western blot - Anti-CD9 antibody [EM-04] (ab82390), expandable thumbnail](https://content.abcam.com/products/images/cd9-antibody-em-04-ab82390--western-blot-img43155.jpg "Western blot - Anti-CD9 antibody [EM-04] (ab82390)")
Western blot - Anti-CD9 antibody [EM-04] (ab82390)
All lanes: Western blot - Anti-CD9 antibody [EM-04] (ab82390) at 1 µg/mL
Lane 1: lysate from 1X10^6 mouse bone marrow-derived mast cell (BMMC)
Lane 2: lysate from 0.5X10^6 mouse bone marrow-derived mast cell (BMMC)
Lane 3: lysate from 0.25X10^6 mouse bone marrow-derived mast cell (BMMC)
Lane 4: lysate from 0.125X10^6 mouse bone marrow-derived mast cell (BMMC)
Performed under non-reducing conditions.
Predicted band size: 25 kDa
Observed band size: 24 kDa
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