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Anti-CD9 antibody [EPR23105-125] (ab263019) is a rabbit monoclonal antibody detecting CD9 in Western Blot, Flow Cytometry, IP, IHC-P. Suitable for Human.



- KO validated for confirmed specificity

- Biophysical QC for unrivalled batch-batch consistency

- Over 20 publications

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody [EPR23105-125] (AB263019), expandable thumbnail
  • Flow Cytometry - Anti-CD9 antibody [EPR23105-125] (AB263019), expandable thumbnail
  • Western blot - Anti-CD9 antibody [EPR23105-125] (AB263019), expandable thumbnail
  • Immunoprecipitation - Anti-CD9 antibody [EPR23105-125] (AB263019), expandable thumbnail
  • Western blot - Anti-CD9 antibody [EPR23105-125] (AB263019), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBICC/IFIHC-P
Human
Tested
Tested
Tested
Not recommended
Tested

Tested
Tested

Species
Human
Dilution info
1/30
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

Integral membrane protein associated with integrins, which regulates different processes, such as sperm-egg fusion, platelet activation and aggregation, and cell adhesion (PubMed:14575715, PubMed:18541721, PubMed:8478605). Present at the cell surface of oocytes and plays a key role in sperm-egg fusion, possibly by organizing multiprotein complexes and the morphology of the membrane required for the fusion (By similarity). In myoblasts, associates with CD81 and PTGFRN and inhibits myotube fusion during muscle regeneration (By similarity). In macrophages, associates with CD81 and beta-1 and beta-2 integrins, and prevents macrophage fusion into multinucleated giant cells specialized in ingesting complement-opsonized large particles (PubMed:12796480). Also prevents the fusion between mononuclear cell progenitors into osteoclasts in charge of bone resorption (By similarity). Acts as a receptor for PSG17 (By similarity). Involved in platelet activation and aggregation (PubMed:18541721). Regulates paranodal junction formation (By similarity). Involved in cell adhesion, cell motility and tumor metastasis (PubMed:7511626, PubMed:8478605).

Alternative names

CD9, MIC3, TSPAN29, GIG2, CD9 antigen, 5H9 antigen, Cell growth-inhibiting gene 2 protein, Leukocyte antigen MIC3, Motility-related protein, Tetraspanin-29, p24, MRP-1, Tspan-29

Recommended products

Anti-CD9 antibody [EPR23105-125] (ab263019) is a rabbit monoclonal antibody detecting CD9 in Western Blot, Flow Cytometry, IP, IHC-P. Suitable for Human.



- KO validated for confirmed specificity

- Biophysical QC for unrivalled batch-batch consistency

- Over 20 publications

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR23105-125
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Product Specifications

Anti-CD9 antibody [EPR23105-125] (ab263019) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt, IHC-P, IP, WB in human samples.
Anti-CD9 antibody [EPR23105-125] (ab263019) specifically detects CD9 (UniProt ID: P21926; Molecular weight: 25kDa) and is sold in 100 µL and 1 mL selling sizes.

Quality and Validation

Abcam's high quality manufacturing and validation processes ensure Anti-CD9 antibody [EPR23105-125] (ab263019) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
The specificity of Anti-CD9 antibody [EPR23105-125] (ab263019) has been confirmed by testing in knockout samples.
Anti-CD9 antibody [EPR23105-125] (ab263019) has been cited over 22 times in peer reviewed journals and is trusted by the scientific community.

Related Products
Conjugation-ready, carrier free format available for antibody clone EPR23105-125 - Anti-CD9 antibody [EPR23105-125] - BSA and Azide free ab263024.
Antibody clone EPR23105-125 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 488 (Alexa Fluor® 488 Anti-CD9 antibody [EPR23105-125] ab316875).
Target Information
CD9 also known as p24 is a member of the tetraspanin family, which includes proteins involved in various cellular processes. CD9 (p24) plays a significant role in cancer progression and metastasis by influencing cell adhesion, migration, and signaling. CD9 (p24) is found on the surface of cancer cells and within exosomes, impacting cancer progression and therapy resistance. CD9's role in cancer is complex, as it can both suppress and promote tumor growth depending on the context. The protein's interactions within tetraspanin-enriched microdomains (TEMs) are crucial for its function.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CD9 also known as motility-related protein-1 (MRP-1) is a transmembrane glycoprotein with a molecular weight of approximately 24 kDa. This protein is part of the tetraspanin family and shows widespread expression throughout different tissues including hematopoietic and non-hematopoietic cells. CD9 participates in numerous cellular processes by interacting with integrins and other cell surface receptors facilitating the organization of molecular complexes within the cell membrane.

Biological function summary

The CD9 protein is integral to cell adhesion migration and fusion. It forms complexes with other tetraspanins and proteins like EWI-2 and EWI-F contributing to cellular signaling and membrane compartmentalization. Additionally CD9 plays an important role in the formation and secretion of exosomes tiny vesicles emitted by cells that mediate cell-to-cell communication. These exosomes can be analyzed using tools like exosome assays and detection kits that target CD9 proteins.

Pathways

CD9 is involved in the regulation of immune response and cell morphology. It participates in pathways such as the integrin signaling pathway and the phosphatidylinositol 3-kinase (PI3K) signaling pathway. CD9 modulates these interactions by associating with proteins such as integrins and other members of the tetraspanin family influencing cellular movement and proliferation.

Associated diseases and disorders

CD9 has connections to various conditions including cancer and infectious diseases. In cancer CD9 can influence tumor progression and metastasis while its altered expression levels have been associated with different cancer types. CD9 interacts with proteins such as integrins and CD81 in these contexts affecting cellular adhesion and migration mechanisms. In infectious diseases CD9 is involved in viral entry processes as some viruses utilize CD9 and its associated tetraspanins for entry into host cells.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody [EPR23105-125] (ab263019), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody [EPR23105-125] (ab263019)

    Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling CD9 with ab263019 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on human cervical carcinoma. The section was incubated with ab263019 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

  • Flow Cytometry - Anti-CD9 antibody [EPR23105-125] (ab263019), expandable thumbnail

    Flow Cytometry - Anti-CD9 antibody [EPR23105-125] (ab263019)

    Flow cytometric analysis of Raji (human Burkitt's lymphoma B lymphocyte, Left) / HCT 116 (human colorectal carcinoma epithelial cell, Right) cells labelling CD9 with ab263019 at 1/500 dilution compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 was used as the secondary antibody.

    Negative control: Raji (PMID: 8921952).

    Gated on viable cells.

  • Western blot - Anti-CD9 antibody [EPR23105-125] (ab263019), expandable thumbnail

    Western blot - Anti-CD9 antibody [EPR23105-125] (ab263019)

    Lanes 1 - 4: Merged signal (red and green). Green - ab263019 observed at 18 kDa. Red - loading control Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.

    ab263019 was shown to react with CD9 in wild-type HeLa cells in Western blot with loss of signal observed in CD9 knockout cell line Human CD9 knockout HeLa cell line ab255375 (CD9 knockout cell lysate Human CD9 knockout HeLa cell lysate ab263754). Wild-type HeLa and CD9 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab263019 and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

    All lanes: Western blot - Anti-CD9 antibody [EPR23105-125] (ab263019) at 1/1000 dilution

    Lane 1: Wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

    Lane 2: CD9 knockout HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

    Lane 2: Western blot - Human CD9 knockout HeLa cell line (Human CD9 knockout HeLa cell line ab255375)

    Lane 3: A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

    Lane 4: MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 25 kDa

    Observed band size: 18 kDa

  • Immunoprecipitation - Anti-CD9 antibody [EPR23105-125] (ab263019), expandable thumbnail

    Immunoprecipitation - Anti-CD9 antibody [EPR23105-125] (ab263019)

    CD9 was immunoprecipitated from 0.35 mg HCT 116 (human colorectal carcinoma epithelial cell) whole cell lysate with ab263019 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab263019 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/1000 dilution.

    Lane 1: HCT 116 (human colorectal carcinoma epithelial cell) whole cell lysate 10ug.

    Lane 2: ab263019 IP in HCT 116 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab263019 in HCT 116 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

    All lanes: Immunoprecipitation - Anti-CD9 antibody [EPR23105-125] (ab263019)

    Predicted band size: 25 kDa

    Observed band size: 22 kDa

  • Western blot - Anti-CD9 antibody [EPR23105-125] (ab263019), expandable thumbnail

    Western blot - Anti-CD9 antibody [EPR23105-125] (ab263019)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 3275469). Exposure time: 3 minutes.

    All lanes: Western blot - Anti-CD9 antibody [EPR23105-125] (ab263019) at 1/1000 dilution

    Lane 1: HEL (erythroleukemia erythroblast), whole cell lysate at 20 µg

    Lane 2: Human tonsil lysate at 20 µg

    Lane 3: Human colon lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 25 kDa

    Observed band size: 22 kDa

  • Western blot - Anti-CD9 antibody [EPR23105-125] (ab263019), expandable thumbnail

    Western blot - Anti-CD9 antibody [EPR23105-125] (ab263019)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 3275469).

    Negative control: Raji (PMID: 8921952). Exposure time: 3 minutes.

    All lanes: Western blot - Anti-CD9 antibody [EPR23105-125] (ab263019) at 1/1000 dilution

    Lane 1: HCT 116 (human colorectal carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: Raji (human Burkitts lymphoma B lymphocyte) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 25 kDa

    Observed band size: 22 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody [EPR23105-125] (ab263019), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody [EPR23105-125] (ab263019)

    Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling CD9 with ab263019 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on platelets of human spleen. The section was incubated with ab263019 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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