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AB263019

Anti-CD9 antibody [EPR23105-125]

  • BOND RX™ Validated
  • RabMAb
  • Recombinant
  • KO Validated
  • What is this?

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(64 Publications)

Anti-CD9 antibody [EPR23105-125] (ab263019) is a rabbit monoclonal antibody detecting CD9 in Western Blot, Flow Cytometry, IP, IHC-P. Suitable for Human.

- KO validated for confirmed specificity
- Biophysical QC for unrivalled batch-batch consistency
- Over 20 publications

View Alternative Names

CD9, MIC3, TSPAN29, GIG2, CD9 antigen, 5H9 antigen, Cell growth-inhibiting gene 2 protein, Leukocyte antigen MIC3, Motility-related protein, Tetraspanin-29, p24, MRP-1, Tspan-29

7 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody [EPR23105-125] (AB263019)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody [EPR23105-125] (AB263019)

Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling CD9 with ab263019 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human cervical carcinoma. The section was incubated with ab263019 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

Flow Cytometry - Anti-CD9 antibody [EPR23105-125] (AB263019)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-CD9 antibody [EPR23105-125] (AB263019)

Flow cytometric analysis of Raji (human Burkitt's lymphoma B lymphocyte, Left) / HCT 116 (human colorectal carcinoma epithelial cell, Right) cells labelling CD9 with ab263019 at 1/500 dilution compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody.

Negative control : Raji (PMID : 8921952).

Gated on viable cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody [EPR23105-125] (AB263019)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody [EPR23105-125] (AB263019)

Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling CD9 with ab263019 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on platelets of human spleen. The section was incubated with ab263019 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

Immunoprecipitation - Anti-CD9 antibody [EPR23105-125] (AB263019)
  • IP

Unknown

Immunoprecipitation - Anti-CD9 antibody [EPR23105-125] (AB263019)

CD9 was immunoprecipitated from 0.35 mg HCT 116 (human colorectal carcinoma epithelial cell) whole cell lysate with ab263019 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab263019 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.

Lane 1 : HCT 116 (human colorectal carcinoma epithelial cell) whole cell lysate 10ug.

Lane 2 : ab263019 IP in HCT 116 whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab263019 in HCT 116 whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 10 seconds.

All lanes:

Immunoprecipitation - Anti-CD9 antibody [EPR23105-125] (ab263019)

Predicted band size: 25 kDa

Observed band size: 22 kDa

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Western blot - Anti-CD9 antibody [EPR23105-125] (AB263019)
  • WB

Lab

Western blot - Anti-CD9 antibody [EPR23105-125] (AB263019)

Lanes 1 - 4 : Merged signal (red and green). Green - ab263019 observed at 18 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.

ab263019 was shown to react with CD9 in wild-type HeLa cells in Western blot with loss of signal observed in CD9 knockout cell line ab255375 (CD9 knockout cell lysate ab263754). Wild-type HeLa and CD9 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab263019 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-CD9 antibody [EPR23105-125] (ab263019) at 1/1000 dilution

Lane 1:

Wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 2:

CD9 knockout HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 2:

Western blot - Human CD9 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-cd9-knockout-hela-cell-line-ab255375'>ab255375</a>)

Lane 3:

A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

Lane 4:

MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg

Predicted band size: 25 kDa

Observed band size: 18 kDa

false

Western blot - Anti-CD9 antibody [EPR23105-125] (AB263019)
  • WB

Lab

Western blot - Anti-CD9 antibody [EPR23105-125] (AB263019)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID : 3275469). Exposure time : 3 minutes.

All lanes:

Western blot - Anti-CD9 antibody [EPR23105-125] (ab263019) at 1/1000 dilution

Lane 1:

HEL (erythroleukemia erythroblast), whole cell lysate at 20 µg

Lane 2:

Human tonsil lysate at 20 µg

Lane 3:

Human colon lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 25 kDa

Observed band size: 22 kDa

false

Western blot - Anti-CD9 antibody [EPR23105-125] (AB263019)
  • WB

Lab

Western blot - Anti-CD9 antibody [EPR23105-125] (AB263019)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID : 3275469).

Negative control : Raji (PMID : 8921952). Exposure time : 3 minutes.

All lanes:

Western blot - Anti-CD9 antibody [EPR23105-125] (ab263019) at 1/1000 dilution

Lane 1:

HCT 116 (human colorectal carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

Raji (human Burkitts lymphoma B lymphocyte) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 25 kDa

Observed band size: 22 kDa

false

  • Carrier free

    Anti-CD9 antibody [EPR23105-125] - BSA and Azide free

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-CD9 antibody [EPR23105-125]

  • 578 PE

    PE Anti-CD9 antibody [EPR23105-125]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23105-125

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Flow Cyt, IHC-P, WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Alternative Products

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/500", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p>" } } }
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Product details

Product Specifications

Anti-CD9 antibody [EPR23105-125] (ab263019) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt, IHC-P, IP, WB in human samples.
Anti-CD9 antibody [EPR23105-125] (ab263019) specifically detects CD9 (UniProt ID: P21926; Molecular weight: 25kDa) and is sold in 100 µL and 1 mL selling sizes.

Quality and Validation

Abcam's high quality manufacturing and validation processes ensure Anti-CD9 antibody [EPR23105-125] (ab263019) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
The specificity of Anti-CD9 antibody [EPR23105-125] (ab263019) has been confirmed by testing in knockout samples.
Anti-CD9 antibody [EPR23105-125] (ab263019) has been cited over 22 times in peer reviewed journals and is trusted by the scientific community.

Related Products
Conjugation-ready, carrier free format available for antibody clone EPR23105-125 - ab263024.
Antibody clone EPR23105-125 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 488 (ab316875).
Target Information
CD9 also known as p24 is a member of the tetraspanin family, which includes proteins involved in various cellular processes. CD9 (p24) plays a significant role in cancer progression and metastasis by influencing cell adhesion, migration, and signaling. CD9 (p24) is found on the surface of cancer cells and within exosomes, impacting cancer progression and therapy resistance. CD9's role in cancer is complex, as it can both suppress and promote tumor growth depending on the context. The protein's interactions within tetraspanin-enriched microdomains (TEMs) are crucial for its function.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD9 also known as motility-related protein-1 (MRP-1) is a transmembrane glycoprotein with a molecular weight of approximately 24 kDa. This protein is part of the tetraspanin family and shows widespread expression throughout different tissues including hematopoietic and non-hematopoietic cells. CD9 participates in numerous cellular processes by interacting with integrins and other cell surface receptors facilitating the organization of molecular complexes within the cell membrane.
Biological function summary

The CD9 protein is integral to cell adhesion migration and fusion. It forms complexes with other tetraspanins and proteins like EWI-2 and EWI-F contributing to cellular signaling and membrane compartmentalization. Additionally CD9 plays an important role in the formation and secretion of exosomes tiny vesicles emitted by cells that mediate cell-to-cell communication. These exosomes can be analyzed using tools like exosome assays and detection kits that target CD9 proteins.

Pathways

CD9 is involved in the regulation of immune response and cell morphology. It participates in pathways such as the integrin signaling pathway and the phosphatidylinositol 3-kinase (PI3K) signaling pathway. CD9 modulates these interactions by associating with proteins such as integrins and other members of the tetraspanin family influencing cellular movement and proliferation.

CD9 has connections to various conditions including cancer and infectious diseases. In cancer CD9 can influence tumor progression and metastasis while its altered expression levels have been associated with different cancer types. CD9 interacts with proteins such as integrins and CD81 in these contexts affecting cellular adhesion and migration mechanisms. In infectious diseases CD9 is involved in viral entry processes as some viruses utilize CD9 and its associated tetraspanins for entry into host cells.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Integral membrane protein associated with integrins, which regulates different processes, such as sperm-egg fusion, platelet activation and aggregation, and cell adhesion (PubMed : 14575715, PubMed : 18541721, PubMed : 8478605). Present at the cell surface of oocytes and plays a key role in sperm-egg fusion, possibly by organizing multiprotein complexes and the morphology of the membrane required for the fusion (By similarity). In myoblasts, associates with CD81 and PTGFRN and inhibits myotube fusion during muscle regeneration (By similarity). In macrophages, associates with CD81 and beta-1 and beta-2 integrins, and prevents macrophage fusion into multinucleated giant cells specialized in ingesting complement-opsonized large particles (PubMed : 12796480). Also prevents the fusion between mononuclear cell progenitors into osteoclasts in charge of bone resorption (By similarity). Acts as a receptor for PSG17 (By similarity). Involved in platelet activation and aggregation (PubMed : 18541721). Regulates paranodal junction formation (By similarity). Involved in cell adhesion, cell motility and tumor metastasis (PubMed : 7511626, PubMed : 8478605).
See full target information CD9
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Publications (64)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed41009408

2025

Extracellular Vesicles Derived from Human Umbilical Cord Mesenchymal Stem Cells Alleviated the Inflammatory Response in Mice Infected with the Influenza Virus A (H1N1).

Applications

Unspecified application

Species

Unspecified reactive species

Hui Xiao,Xiao Yu,Yiding Dong,Shilong Bao,Xiaoting Meng,Jia Zhao,Zhiyong Dong

Biology 14: PubMed40906403

2025

Study of the Therapeutic Effect of Cytokine-Preconditioned Mesenchymal Stem Cells and Their Exosomes in a Mouse Model of Psoriasis.

Applications

Unspecified application

Species

Unspecified reactive species

Aidar Dairov,Assel Issabekova,Madina Sarsenova,Aliya Sekenova,Miras Shakhatbayev,Symbat Alimbek,Gulshakhar Kudaibergen,Assiya Nurkina,Ilyas Akhmetollayev,Kyung-Sun Kang,Vyacheslav Ogay

Molecular medicine (Cambridge, Mass.) 31:278 PubMed40819077

2025

Targeting LINC02544/miR-497-5p/CAPRIN1 axis via exosome-based siRNA to overcome immunotherapy resistance in triple-negative breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Lian,Jiayi Li,Shihui Tang,Ting Li,Jinping Li

Scientific reports 15:29273 PubMed40784956

2025

CAFs exosomal circFOXO1 promotes TNBC autophagy and radioresistance via miR-27a-3p/BNIP3 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xueqiong Xun,Huiyong Hu,Qing Liu,Ruijun Su,Jun Ai

Journal of cellular and molecular medicine 29:e70725 PubMed40770945

2025

Deciphering the Proteomic Landscape of Circulating Extracellular Vesicles in Human Abdominal Aortic Aneurysm.

Applications

Unspecified application

Species

Unspecified reactive species

Chaoyang Yu,Ge Zhang,Shaotong Pei,Yifei Zhang,Peiyu Yuan,Renying Miao,Kaisaierjiang Kadier,Pengpeng Zhang,Tianshu Gu,Ruhao Wu,Haonan Zhang,Shiqian Zhang,Bo Yang,Han Wu,Yudi Xu,Ke Hu,Qingfei Xu,Yaxin Chen,Jinliang Wang,Zongao Cai,Junnan Tang,Teng Li,Yan Song

Journal of extracellular vesicles 14:e70099 PubMed40545968

2025

Engineered Mesenchymal Stem Cell-Derived Extracellular Vesicles Reverse Endothelial-Mesenchymal Transition in Atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhuo Chen,Kai Chang,Sha Yang,Ruijia Deng,Yuan He,Ligai Zhang,Ming Chen,Dingqun Bai,Yan Pi

Cell biology and toxicology 41:103 PubMed40515797

2025

GelMA hydrogel-loaded extracellular vesicles derived from keratinocytes promote skin microvasculature regeneration and wound healing in diabetic mice through activation of the PDGF-induced PI3K/AKT pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Li,Leilei Zhou,Wenqiang Li,Weiheng Zhao,Weimin Chen,Mohammed S AlQranei,Jiarui Bi,Ping Huang

Cell and tissue research 401:275-286 PubMed40481270

2025

Proteomic analysis of secreted proteins derived from amniotic fluid stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Tatsanee Phermthai,Puttachart Chuaynarong,Suparat Wichitwiengrat,Kamonpat Phermthai,Sittiruk Roytrakul,Thanuch Chitthira,Sasiprapa Thongbopit

The Journal of cell biology 224: PubMed40304687

2025

Extracellular vesicles adhere to cells primarily by interactions of integrins and GM1 with laminin.

Applications

Unspecified application

Species

Unspecified reactive species

Tatsuki Isogai,Koichiro M Hirosawa,Miki Kanno,Ayano Sho,Rinshi S Kasai,Naoko Komura,Hiromune Ando,Keiko Furukawa,Yuhsuke Ohmi,Koichi Furukawa,Yasunari Yokota,Kenichi G N Suzuki

Acta pharmaceutica Sinica. B 15:1007-1022 PubMed40177564

2025

Extracellular vesicles deliver thioredoxin to rescue stem cells from senescence and intervertebral disc degeneration a feed-forward circuit of the NRF2/AP-1 composite pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xuanzuo Chen,Sheng Liu,Huiwen Wang,Yiran Liu,Yan Xiao,Kanglu Li,Feifei Ni,Wei Wu,Hui Lin,Xiangcheng Qing,Feifei Pu,Baichuan Wang,Zengwu Shao,Yizhong Peng
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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