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Rabbit Recombinant Monoclonal CD9 antibody. Suitable for IP, Flow Cyt, WB, IHC-P and reacts with Human samples. Cited in 22 publications.

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Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBICC/IFIHC-P
Human
Tested
Tested
Tested
Not recommended
Tested

Tested
Tested

Species

Human

Dilution info

1/30

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

1/500

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

Function

Integral membrane protein associated with integrins, which regulates different processes, such as sperm-egg fusion, platelet activation and aggregation, and cell adhesion (PubMed:8478605, PubMed:14575715, PubMed:18541721). Present at the cell surface of oocytes and plays a key role in sperm-egg fusion, possibly by organizing multiprotein complexes and the morphology of the membrane required for the fusion (By similarity). In myoblasts, associates with CD81 and PTGFRN and inhibits myotube fusion during muscle regeneration (By similarity). In macrophages, associates with CD81 and beta-1 and beta-2 integrins, and prevents macrophage fusion into multinucleated giant cells specialized in ingesting complement-opsonized large particles (PubMed:12796480). Also prevents the fusion between mononuclear cell progenitors into osteoclasts in charge of bone resorption (By similarity). Acts as a receptor for PSG17 (By similarity). Involved in platelet activation and aggregation (PubMed:18541721). Regulates paranodal junction formation (By similarity). Involved in cell adhesion, cell motility and tumor metastasis (PubMed:8478605, PubMed:7511626).

Alternative names

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Rabbit Recombinant Monoclonal CD9 antibody. Suitable for IP, Flow Cyt, WB, IHC-P and reacts with Human samples. Cited in 22 publications.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR23105-125

Purification technique

Affinity purification Protein A

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

Biological function summary

The CD9 protein is integral to cell adhesion migration and fusion. It forms complexes with other tetraspanins and proteins like EWI-2 and EWI-F contributing to cellular signaling and membrane compartmentalization. Additionally CD9 plays an important role in the formation and secretion of exosomes tiny vesicles emitted by cells that mediate cell-to-cell communication. These exosomes can be analyzed using tools like exosome assays and detection kits that target CD9 proteins.

Activity summary

CD9 also known as motility-related protein-1 (MRP-1) is a transmembrane glycoprotein with a molecular weight of approximately 24 kDa. This protein is part of the tetraspanin family and shows widespread expression throughout different tissues including hematopoietic and non-hematopoietic cells. CD9 participates in numerous cellular processes by interacting with integrins and other cell surface receptors facilitating the organization of molecular complexes within the cell membrane.

Pathways

CD9 is involved in the regulation of immune response and cell morphology. It participates in pathways such as the integrin signaling pathway and the phosphatidylinositol 3-kinase (PI3K) signaling pathway. CD9 modulates these interactions by associating with proteins such as integrins and other members of the tetraspanin family influencing cellular movement and proliferation.

Associated diseases and disorders

CD9 has connections to various conditions including cancer and infectious diseases. In cancer CD9 can influence tumor progression and metastasis while its altered expression levels have been associated with different cancer types. CD9 interacts with proteins such as integrins and CD81 in these contexts affecting cellular adhesion and migration mechanisms. In infectious diseases CD9 is involved in viral entry processes as some viruses utilize CD9 and its associated tetraspanins for entry into host cells.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Flow Cytometry - Anti-CD9 antibody [EPR23105-125] (ab263019), expandable thumbnail

    Flow Cytometry - Anti-CD9 antibody [EPR23105-125] (ab263019)

    Flow cytometric analysis of Raji (human Burkitt's lymphoma B lymphocyte, Left) / HCT 116 (human colorectal carcinoma epithelial cell, Right) cells labelling CD9 with ab263019 at 1/500 dilution compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody.

    Negative control: Raji (PMID: 8921952).

    Gated on viable cells.

  • Western blot - Anti-CD9 antibody [EPR23105-125] (ab263019), expandable thumbnail

    Western blot - Anti-CD9 antibody [EPR23105-125] (ab263019)

    Lanes 1 - 4: Merged signal (red and green). Green - ab263019 observed at 18 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.

    ab263019 was shown to react with CD9 in wild-type HeLa cells in Western blot with loss of signal observed in CD9 knockout cell line ab255375 (CD9 knockout cell lysate ab263754). Wild-type HeLa and CD9 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab263019 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

    All lanes: Western blot - Anti-CD9 antibody [EPR23105-125] (AB263019) at 1/1000 dilution

    Lane 1: Wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

    Lane 2: CD9 knockout HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

    Lane 3: A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

    Lane 4: MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 25 kDa

    Observed band size: 18 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody [EPR23105-125] (ab263019), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody [EPR23105-125] (ab263019)

    Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling CD9 with ab263019 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human cervical carcinoma. The section was incubated with ab263019 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

  • Immunoprecipitation - Anti-CD9 antibody [EPR23105-125] (ab263019), expandable thumbnail

    Immunoprecipitation - Anti-CD9 antibody [EPR23105-125] (ab263019)

    CD9 was immunoprecipitated from 0.35 mg HCT 116 (human colorectal carcinoma epithelial cell) whole cell lysate with ab263019 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab263019 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.

    Lane 1: HCT 116 (human colorectal carcinoma epithelial cell) whole cell lysate 10ug.

    Lane 2: ab263019 IP in HCT 116 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab263019 in HCT 116 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

    All lanes: Immunoprecipitation - Anti-CD9 antibody [EPR23105-125] (AB263019)

    Predicted band size: 25 kDa

    Observed band size: 22 kDa

  • Western blot - Anti-CD9 antibody [EPR23105-125] (ab263019), expandable thumbnail

    Western blot - Anti-CD9 antibody [EPR23105-125] (ab263019)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 3275469). Exposure time: 3 minutes.

    All lanes: Western blot - Anti-CD9 antibody [EPR23105-125] (AB263019) at 1/1000 dilution

    Lane 1: HEL (erythroleukemia erythroblast), whole cell lysate at 20 µg

    Lane 2: Human tonsil lysate at 20 µg

    Lane 3: Human colon lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/100000 dilution

    Predicted band size: 25 kDa

    Observed band size: 22 kDa

  • Western blot - Anti-CD9 antibody [EPR23105-125] (ab263019), expandable thumbnail

    Western blot - Anti-CD9 antibody [EPR23105-125] (ab263019)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 3275469).

    Negative control: Raji (PMID: 8921952). Exposure time: 3 minutes.

    All lanes: Western blot - Anti-CD9 antibody [EPR23105-125] (AB263019) at 1/1000 dilution

    Lane 1: HCT 116 (human colorectal carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: Raji (human Burkitts lymphoma B lymphocyte) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/100000 dilution

    Predicted band size: 25 kDa

    Observed band size: 22 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody [EPR23105-125] (ab263019), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody [EPR23105-125] (ab263019)

    Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling CD9 with ab263019 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on platelets of human spleen. The section was incubated with ab263019 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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