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Rabbit Recombinant Monoclonal CD9 antibody. Carrier free. Suitable for IHC-P, WB, IP and reacts with Mouse, Rat, Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody [EPR27551-92] - BSA and Azide free (AB307086), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody [EPR27551-92] - BSA and Azide free (AB307086), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody [EPR27551-92] - BSA and Azide free (AB307086), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody [EPR27551-92] - BSA and Azide free (AB307086), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody [EPR27551-92] - BSA and Azide free (AB307086), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIHC-FrICC/IFFlow CytWBIP
Human
Not recommended
Not recommended
Not recommended
Not recommended
Tested
Expected
Mouse
Tested
Not recommended
Not recommended
Not recommended
Tested
Tested
Rat
Tested
Not recommended
Not recommended
Not recommended
Tested
Expected

Tested
Tested

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

This antibody is not suitable for human species in IHC-P application.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

-

Notes

-

Expected
Expected

Species

Rat, Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

1 products for Alternative Version

Target data

Function

Integral membrane protein associated with integrins, which regulates different processes, such as sperm-egg fusion, platelet activation and aggregation, and cell adhesion (PubMed:8478605, PubMed:14575715, PubMed:18541721). Present at the cell surface of oocytes and plays a key role in sperm-egg fusion, possibly by organizing multiprotein complexes and the morphology of the membrane required for the fusion (By similarity). In myoblasts, associates with CD81 and PTGFRN and inhibits myotube fusion during muscle regeneration (By similarity). In macrophages, associates with CD81 and beta-1 and beta-2 integrins, and prevents macrophage fusion into multinucleated giant cells specialized in ingesting complement-opsonized large particles (PubMed:12796480). Also prevents the fusion between mononuclear cell progenitors into osteoclasts in charge of bone resorption (By similarity). Acts as a receptor for PSG17 (By similarity). Involved in platelet activation and aggregation (PubMed:18541721). Regulates paranodal junction formation (By similarity). Involved in cell adhesion, cell motility and tumor metastasis (PubMed:8478605, PubMed:7511626).

Alternative names

CD9 antigen, 5H9 antigen, Cell growth-inhibiting gene 2 protein, Leukocyte antigen MIC3, Motility-related protein, Tetraspanin-29, p24, MRP-1, Tspan-29, MIC3, TSPAN29, GIG2, CD9

Recommended products

Rabbit Recombinant Monoclonal CD9 antibody. Carrier free. Suitable for IHC-P, WB, IP and reacts with Mouse, Rat, Human samples.

Alternative names

CD9 antigen, 5H9 antigen, Cell growth-inhibiting gene 2 protein, Leukocyte antigen MIC3, Motility-related protein, Tetraspanin-29, p24, MRP-1, Tspan-29, MIC3, TSPAN29, GIG2, CD9

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR27551-92

Purification technique

Affinity purification Protein A

Specificity

Unsuitable for human IHC-P.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Notes

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CD9 also known as motility-related protein-1 (MRP-1) is a transmembrane glycoprotein with a molecular weight of approximately 24 kDa. This protein is part of the tetraspanin family and shows widespread expression throughout different tissues including hematopoietic and non-hematopoietic cells. CD9 participates in numerous cellular processes by interacting with integrins and other cell surface receptors facilitating the organization of molecular complexes within the cell membrane.

Biological function summary

The CD9 protein is integral to cell adhesion migration and fusion. It forms complexes with other tetraspanins and proteins like EWI-2 and EWI-F contributing to cellular signaling and membrane compartmentalization. Additionally CD9 plays an important role in the formation and secretion of exosomes tiny vesicles emitted by cells that mediate cell-to-cell communication. These exosomes can be analyzed using tools like exosome assays and detection kits that target CD9 proteins.

Pathways

CD9 is involved in the regulation of immune response and cell morphology. It participates in pathways such as the integrin signaling pathway and the phosphatidylinositol 3-kinase (PI3K) signaling pathway. CD9 modulates these interactions by associating with proteins such as integrins and other members of the tetraspanin family influencing cellular movement and proliferation.

Associated diseases and disorders

CD9 has connections to various conditions including cancer and infectious diseases. In cancer CD9 can influence tumor progression and metastasis while its altered expression levels have been associated with different cancer types. CD9 interacts with proteins such as integrins and CD81 in these contexts affecting cellular adhesion and migration mechanisms. In infectious diseases CD9 is involved in viral entry processes as some viruses utilize CD9 and its associated tetraspanins for entry into host cells.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody [EPR27551-92] - BSA and Azide free (ab307086), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody [EPR27551-92] - BSA and Azide free (ab307086)

    This data was developed using Anti-CD9 antibody [EPR27551-92] ab307085, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling CD9 with Anti-CD9 antibody [EPR27551-92] ab307085 at 1/2000 (0.228 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Membranous staining in rat kidney. The section was incubated with Anti-CD9 antibody [EPR27551-92] ab307085 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody [EPR27551-92] - BSA and Azide free (ab307086), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody [EPR27551-92] - BSA and Azide free (ab307086)

    This data was developed using Anti-CD9 antibody [EPR27551-92] ab307085, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling CD9 with Anti-CD9 antibody [EPR27551-92] ab307085 at 1/2000 (0.228 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining in rat cerebrum. The section was incubated with Anti-CD9 antibody [EPR27551-92] ab307085 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody [EPR27551-92] - BSA and Azide free (ab307086), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody [EPR27551-92] - BSA and Azide free (ab307086)

    This data was developed using Anti-CD9 antibody [EPR27551-92] ab307085, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse lung cancer tissue labeling CD9 with Anti-CD9 antibody [EPR27551-92] ab307085 at 1/2000 (0.228 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Membranous staining in mouse lung cancer. The section was incubated with Anti-CD9 antibody [EPR27551-92] ab307085 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody [EPR27551-92] - BSA and Azide free (ab307086), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody [EPR27551-92] - BSA and Azide free (ab307086)

    This data was developed using Anti-CD9 antibody [EPR27551-92] ab307085, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling CD9 with Anti-CD9 antibody [EPR27551-92] ab307085 at 1/2000 (0.228 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining in mouse kidney. The section was incubated with Anti-CD9 antibody [EPR27551-92] ab307085 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody [EPR27551-92] - BSA and Azide free (ab307086), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody [EPR27551-92] - BSA and Azide free (ab307086)

    This data was developed using Anti-CD9 antibody [EPR27551-92] ab307085, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling CD9 with Anti-CD9 antibody [EPR27551-92] ab307085 at 1/2000 (0.228 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining in mouse cerebrum. The section was incubated with Anti-CD9 antibody [EPR27551-92] ab307085 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunoprecipitation - Anti-CD9 antibody [EPR27551-92] - BSA and Azide free (ab307086), expandable thumbnail

    Immunoprecipitation - Anti-CD9 antibody [EPR27551-92] - BSA and Azide free (ab307086)

    This data was developed using Anti-CD9 antibody [EPR27551-92] ab307085, the same antibody clone in a different buffer formulation.
    CD9 was immunoprecipitated from 0.35 mg RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate 10 μg with Anti-CD9 antibody [EPR27551-92] ab307085 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-CD9 antibody [EPR27551-92] ab307085 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate 10 μg

    Lane 2: Anti-CD9 antibody [EPR27551-92] ab307085 IP in RAW 264.7 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-CD9 antibody [EPR27551-92] ab307085 in RAW 264.7 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds

    All lanes: Immunoprecipitation - Anti-CD9 antibody [EPR27551-92] (Anti-CD9 antibody [EPR27551-92] ab307085) at 1/30 dilution

    All lanes: RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage)

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Observed band size: 23 kDa

    Exposure time: 10s

    CD9 was immunoprecipitated from 0.35 mg RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate 10 μg with Anti-CD9 antibody [EPR27551-92] ab307085 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-CD9 antibody [EPR27551-92] ab307085 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate 10 μg

    Lane 2: Anti-CD9 antibody [EPR27551-92] ab307085 IP in RAW 264.7 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-CD9 antibody [EPR27551-92] ab307085 in RAW 264.7 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds

  • Western blot - Anti-CD9 antibody [EPR27551-92] - BSA and Azide free (ab307086), expandable thumbnail

    Western blot - Anti-CD9 antibody [EPR27551-92] - BSA and Azide free (ab307086)

    This data was developed using Anti-CD9 antibody [EPR27551-92] ab307085, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration:Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBS The samples were run on a Bis-Tris gel. Performed under reducing conditions. False colour image of Western blot: Anti-CD9 antibody [EPR27551-92] (Anti-CD9 antibody [EPR27551-92] ab307085) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, Anti-CD9 antibody [EPR27551-92] ab307085 was shown to bind specifically to CD9. A band was observed at 23 kDa in wild-type HeLa cell lysates whereas no signal observed at this size in CD9 knockout cell line Human CD9 knockout HeLa cell line ab255375 (knockout cell lysate Human CD9 knockout HeLa cell lysate ab263754). To generate this image, wild-type and CD9 knockout HeLa cell lysates were analyzed. First, samples were run on an SDS-PAGE gel then transferred onto an immobilon-FL PVDF membrane. Membranes were blocked in in Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBS before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.

    All lanes: Western blot - Anti-CD9 antibody [EPR27551-92] (Anti-CD9 antibody [EPR27551-92] ab307085) at 1/1000 dilution

    Lane 1: Wild-type HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: CD9 knockout HeLa whole cell lysate at 20 µg

    Lane 3: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 4: RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

    Secondary

    Lanes 1 - 4: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution

    Lanes 1 - 4: Western blot - Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/10000 dilution

    Observed band size: 23 kDa

    Blocking and diluting buffer and concentration: Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBS The samples were run on a Bis-Tris gel. Performed under reducing conditions. False colour image of Western blot: Anti-CD9 antibody [EPR27551-92] (Anti-CD9 antibody [EPR27551-92] ab307085) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, Anti-CD9 antibody [EPR27551-92] ab307085 was shown to bind specifically to CD9. A band was observed at 23 kDa in wild-type HeLa cell lysates whereas no signal observed at this size in CD9 knockout cell line Human CD9 knockout HeLa cell line ab255375 (knockout cell lysate Human CD9 knockout HeLa cell lysate ab263754). To generate this image, wild-type and CD9 knockout HeLa cell lysates were analyzed. First, samples were run on an SDS-PAGE gel then transferred onto an immobilon-FL PVDF membrane. Membranes were blocked in in Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBS before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.

  • Western blot - Anti-CD9 antibody [EPR27551-92] - BSA and Azide free (ab307086), expandable thumbnail

    Western blot - Anti-CD9 antibody [EPR27551-92] - BSA and Azide free (ab307086)

    This data was developed using Anti-CD9 antibody [EPR27551-92] ab307085, the same antibody clone in a different buffer formulation.
    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Negative controls: K-562, Raji (PMID:17407154 ; PMID: 8921952).
    Exposure time: 70 seconds

    All lanes: Western blot - Anti-CD9 antibody [EPR27551-92] (Anti-CD9 antibody [EPR27551-92] ab307085) at 1/1000 dilution

    Lane 1: HCT 116 (human colorectal carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg

    Lane 3: Raji (human Burkitts lymphoma B lymphocyte) whole cell lysate at 20 µg

    Lane 4: Mouse brain tissue lysate at 20 µg

    Lane 5: Mouse kidney tissue lysate at 20 µg

    Lane 6: C6 (rat glial tumor glial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 23 kDa

    Exposure time: 70s

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Negative controls: K-562, Raji (PMID:17407154 ; PMID: 8921952).
    Exposure time: 70 seconds

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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