Rabbit Recombinant Monoclonal CD9 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Images
![Sandwich ELISA - Anti-CD9 antibody [EPR29303-589] - BSA and Azide free (Capture) (AB322891), expandable thumbnail](https://content.abcam.com/products/images/cd9-antibody-epr29303-589-bsa-and-azide-free-capture-ab322891--sandwich-elisa-img439537.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-CD9 antibody [EPR29303-589] - BSA and Azide free (Capture) (AB322891)")
![Sandwich ELISA - Anti-CD9 antibody [EPR29303-589] - BSA and Azide free (Capture) (AB322891), expandable thumbnail](https://content.abcam.com/products/images/cd9-antibody-epr29303-589-bsa-and-azide-free-capture-ab322891--sandwich-elisa-img439539.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-CD9 antibody [EPR29303-589] - BSA and Azide free (Capture) (AB322891)")
![Sandwich ELISA - Anti-CD9 antibody [EPR29303-589] - BSA and Azide free (Capture) (AB322891), expandable thumbnail](https://content.abcam.com/products/images/cd9-antibody-epr29303-589-bsa-and-azide-free-capture-ab322891--sandwich-elisa-img439538.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-CD9 antibody [EPR29303-589] - BSA and Azide free (Capture) (AB322891)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| sELISA | |
|---|---|
| Human | Tested |
sELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
Integral membrane protein associated with integrins, which regulates different processes, such as sperm-egg fusion, platelet activation and aggregation, and cell adhesion (PubMed:14575715, PubMed:18541721, PubMed:8478605). Present at the cell surface of oocytes and plays a key role in sperm-egg fusion, possibly by organizing multiprotein complexes and the morphology of the membrane required for the fusion (By similarity). In myoblasts, associates with CD81 and PTGFRN and inhibits myotube fusion during muscle regeneration (By similarity). In macrophages, associates with CD81 and beta-1 and beta-2 integrins, and prevents macrophage fusion into multinucleated giant cells specialized in ingesting complement-opsonized large particles (PubMed:12796480). Also prevents the fusion between mononuclear cell progenitors into osteoclasts in charge of bone resorption (By similarity). Acts as a receptor for PSG17 (By similarity). Involved in platelet activation and aggregation (PubMed:18541721). Regulates paranodal junction formation (By similarity). Involved in cell adhesion, cell motility and tumor metastasis (PubMed:7511626, PubMed:8478605).
Alternative names
CD9, MIC3, TSPAN29, GIG2, CD9 antigen, 5H9 antigen, Cell growth-inhibiting gene 2 protein, Leukocyte antigen MIC3, Motility-related protein, Tetraspanin-29, p24, MRP-1, Tspan-29
Rabbit Recombinant Monoclonal CD9 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR29303-589
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab322891 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human CD9. The recommended pair for sandwich ELISA is:
Capture: ab322891, Human CD9 Capture Antibody (unconjugated)
Detector: Anti-CD9 antibody [EPR29303-544] - BSA and Azide free (Detector) ab322892, Human CD9 Detector Antibody (unconjugated)
The reference range value is 0.16-10 ng/mL.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Sandwich ELISA - Anti-CD9 antibody [EPR29303-589] - BSA and Azide free (Capture) (ab322891), expandable thumbnail](https://content.abcam.com/products/images/cd9-antibody-epr29303-589-bsa-and-azide-free-capture-ab322891--sandwich-elisa-img439537.jpg "Sandwich ELISA - Anti-CD9 antibody [EPR29303-589] - BSA and Azide free (Capture) (ab322891)")
Sandwich ELISA - Anti-CD9 antibody [EPR29303-589] - BSA and Azide free (Capture) (ab322891)
Sandwich ELISA of Human CD9 Antibody Pair - BSA and Azide free ab322890 with the capture antibody ab322891 dilution at 2 ug/mL and detector antibody Anti-CD9 antibody [EPR29303-544] - BSA and Azide free (Detector) ab322892 dilution at 0.5 ug/mL.
![Sandwich ELISA - Anti-CD9 antibody [EPR29303-589] - BSA and Azide free (Capture) (ab322891), expandable thumbnail](https://content.abcam.com/products/images/cd9-antibody-epr29303-589-bsa-and-azide-free-capture-ab322891--sandwich-elisa-img439539.jpg "Sandwich ELISA - Anti-CD9 antibody [EPR29303-589] - BSA and Azide free (Capture) (ab322891)")
Sandwich ELISA - Anti-CD9 antibody [EPR29303-589] - BSA and Azide free (Capture) (ab322891)
Interpolated concentration of native CD9 was measured at different sample concentrations. Undiluted sample is as follows: HCT 116 supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- standard deviation, n=2). Sample dilutions are made in 1% bovine serum albumin (BSA). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.
![Sandwich ELISA - Anti-CD9 antibody [EPR29303-589] - BSA and Azide free (Capture) (ab322891), expandable thumbnail](https://content.abcam.com/products/images/cd9-antibody-epr29303-589-bsa-and-azide-free-capture-ab322891--sandwich-elisa-img439538.jpg "Sandwich ELISA - Anti-CD9 antibody [EPR29303-589] - BSA and Azide free (Capture) (ab322891)")
Sandwich ELISA - Anti-CD9 antibody [EPR29303-589] - BSA and Azide free (Capture) (ab322891)
Interpolated concentration of native CD9 was measured at different sample concentrations. Undiluted samples are as follows: human serum 5%, human plasma (EDTA) 50% and human urine 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1% bovine serum albumin (BSA). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.
Downloads
Product protocols
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