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AB255932

Anti-CD9 antibody [P1/33/2] - BSA and Azide free

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Mouse Recombinant Monoclonal CD9 antibody. Carrier free. Suitable for Flow Cyt, ICC/IF and reacts with Human samples.

View Alternative Names

CD9, MIC3, TSPAN29, GIG2, CD9 antigen, 5H9 antigen, Cell growth-inhibiting gene 2 protein, Leukocyte antigen MIC3, Motility-related protein, Tetraspanin-29, p24, MRP-1, Tspan-29

2 Images
Flow Cytometry - Anti-CD9 antibody [P1/33/2] - BSA and Azide free (AB255932)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD9 antibody [P1/33/2] - BSA and Azide free (AB255932)

Flow cytometric analysis of HCT 116 (Human colorectal carcinoma epithelial cell) cells labeling CD9 with ab254175 at 1.006μg/ml (Red) compared with a mouse Isotype control (Black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti mouse IgG (Alexa Fluor® 488, ab150113), at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254175).

Immunocytochemistry/ Immunofluorescence - Anti-CD9 antibody [P1/33/2] - BSA and Azide free (AB255932)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CD9 antibody [P1/33/2] - BSA and Azide free (AB255932)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HCT 116 (human colorectal carcinoma epithelial cell) cells labeling CD9 with ab254175 at 9.66μg/ml, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in HCT 116 cells. The nuclear counterstain is DAPI (Blue). Tubulin is detected with ab179504 anti-beta IV Tubulin antibody - Microtubule Marker at 1/200 dilution, followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary antibody (Red).

Control : Used PBS instead of primary antibody, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution.

Negative control : Raji (PMID : 8921952).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254175).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

P1/33/2

Isotype

IgG1

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1.006 µg/mL", "FlowCyt-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "9.66 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Product details

ab255932 is the carrier-free version of ab254175.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD9 also known as motility-related protein-1 (MRP-1) is a transmembrane glycoprotein with a molecular weight of approximately 24 kDa. This protein is part of the tetraspanin family and shows widespread expression throughout different tissues including hematopoietic and non-hematopoietic cells. CD9 participates in numerous cellular processes by interacting with integrins and other cell surface receptors facilitating the organization of molecular complexes within the cell membrane.
Biological function summary

The CD9 protein is integral to cell adhesion migration and fusion. It forms complexes with other tetraspanins and proteins like EWI-2 and EWI-F contributing to cellular signaling and membrane compartmentalization. Additionally CD9 plays an important role in the formation and secretion of exosomes tiny vesicles emitted by cells that mediate cell-to-cell communication. These exosomes can be analyzed using tools like exosome assays and detection kits that target CD9 proteins.

Pathways

CD9 is involved in the regulation of immune response and cell morphology. It participates in pathways such as the integrin signaling pathway and the phosphatidylinositol 3-kinase (PI3K) signaling pathway. CD9 modulates these interactions by associating with proteins such as integrins and other members of the tetraspanin family influencing cellular movement and proliferation.

CD9 has connections to various conditions including cancer and infectious diseases. In cancer CD9 can influence tumor progression and metastasis while its altered expression levels have been associated with different cancer types. CD9 interacts with proteins such as integrins and CD81 in these contexts affecting cellular adhesion and migration mechanisms. In infectious diseases CD9 is involved in viral entry processes as some viruses utilize CD9 and its associated tetraspanins for entry into host cells.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Integral membrane protein associated with integrins, which regulates different processes, such as sperm-egg fusion, platelet activation and aggregation, and cell adhesion (PubMed : 14575715, PubMed : 18541721, PubMed : 8478605). Present at the cell surface of oocytes and plays a key role in sperm-egg fusion, possibly by organizing multiprotein complexes and the morphology of the membrane required for the fusion (By similarity). In myoblasts, associates with CD81 and PTGFRN and inhibits myotube fusion during muscle regeneration (By similarity). In macrophages, associates with CD81 and beta-1 and beta-2 integrins, and prevents macrophage fusion into multinucleated giant cells specialized in ingesting complement-opsonized large particles (PubMed : 12796480). Also prevents the fusion between mononuclear cell progenitors into osteoclasts in charge of bone resorption (By similarity). Acts as a receptor for PSG17 (By similarity). Involved in platelet activation and aggregation (PubMed : 18541721). Regulates paranodal junction formation (By similarity). Involved in cell adhesion, cell motility and tumor metastasis (PubMed : 7511626, PubMed : 8478605).
See full target information CD9
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com