Anti-CD9 antibody [TS9] - BSA and Azide free

• WB

Lab

Western blot - Anti-CD9 antibody [TS9] - BSA and Azide free (AB58989)

Lanes 1 - 4 : Merged signal (red and green). Green - ab58989 observed at 24 kDa. Red - loading control ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) observed at 55 kDa.

ab58989 was shown to react with CD9 in wild-type HeLa cells in Western blot with loss of signal observed in CD9 knockout cell line ab255375 (CD9 knockout cell lysate ab263754). Wild-type HeLa and CD9 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween^®) before incubation with ab58989 and ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) overnight at 4 °C at 1 μg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye^® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye^® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-CD9 antibody [TS9] - BSA and Azide free (ab58989) at 1 µg/mL

Lane 1:

Wild-type HeLa (No DTT) cell lysate at 20 µg

Lane 2:

CD9 knockout HeLa (No DTT) cell lysate at 20 µg

Lane 2:

Western blot - Human CD9 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-cd9-knockout-hela-cell-line-ab255375'>ab255375</a>)

Lane 3:

A549 (Non-Reducing) cell lysate at 20 µg

Lane 4:

MCF7 (Non-Reducing) cell lysate at 20 µg

Predicted band size: 25 kDa

Observed band size: 24 kDa

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• WB

Supplier Data

Western blot - Anti-CD9 antibody [TS9] - BSA and Azide free (AB58989)

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-CD9 antibody [TS9] - BSA and Azide free (ab58989)

All lanes:

HEL cell lysate at 130000 Cells

Predicted band size: 25 kDa

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