Mouse Monoclonal CD9 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 12 publications. Immunogen corresponding to Cell preparation containing CD9 protein.
Images
![Western blot - Anti-CD9 antibody [TS9] - BSA and Azide free (AB58989), expandable thumbnail](https://content.abcam.com/products/images/cd9-antibody-ts9-bsa-and-azide-free-ab58989--western-blot-img99946.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CD9 antibody [TS9] - BSA and Azide free (AB58989)")
![Western blot - Anti-CD9 antibody [TS9] - BSA and Azide free (AB58989), expandable thumbnail](https://content.abcam.com/products/images/cd9-antibody-ts9-bsa-and-azide-free-ab58989--western-blot-img13713.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CD9 antibody [TS9] - BSA and Azide free (AB58989)")
Publications
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- Proceedings of the National Academy of Sciences of the United States of America 121:e24089491212024Measurement of α-synuclein as protein cargo in plasma extracellular vesicles.Applications:Unspecified applicationReactive species:HumanTal Gilboa,Dmitry Ter-Ovanesyan,Shih-Chin Wang,Sara Whiteman,George T Kannarkat,George M Church,Alice S Chen-Plotkin,David R WaltPubMed 39475636
- Communications biology 6:8852023Selective enrichment of plasma cell-free messenger RNA in cancer-associated extracellular vesicles.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHyun Ji Kim,Matthew J Rames,Florian Goncalves,C Ward Kirschbaum,Breeshey Roskams-Hieter,Elias Spiliotopoulos,Josephine Briand,Aaron Doe,Joseph Estabrook,Josiah T Wagner,Emek Demir,Gordon Mills,Thuy T M NgoPubMed 37644220
- International journal of molecular sciences 24:2023Platelet-Derived Extracellular Vesicles as Lipid Carriers in Severe Allergic Inflammation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlba Couto-Rodriguez,Alma Villaseñor,Carmela Pablo-Torres,David Obeso,María Fernanda Rey-Stolle,Héctor Peinado,José Luis Bueno,Mar Reaño-Martos,Alfredo Iglesias Cadarso,Cristina Gomez-Casado,Coral Barbas,Domingo Barber,María M Escribese,Elena IzquierdoPubMed 37628895
- Cell death discovery 9:2852023Single-cell trajectory analysis reveals a CD9 positive state to contribute to exit from stem cell-like and embryonic diapause states and transit to drug-resistant states.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXi Li,Alfonso Poire,Kang Jin Jeong,Dong Zhang,Gang Chen,Chaoyang Sun,Gordon B MillsPubMed 37542044
- eLife 12:2023Improved isolation of extracellular vesicles by removal of both free proteins and lipoproteins.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDmitry Ter-Ovanesyan,Tal Gilboa,Bogdan Budnik,Adele Nikitina,Sara Whiteman,Roey Lazarovits,Wendy Trieu,David Kalish,George M Church,David R WaltPubMed 37252755
- eLife 10:2021Framework for rapid comparison of extracellular vesicle isolation methods.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDmitry Ter-Ovanesyan,Maia Norman,Roey Lazarovits,Wendy Trieu,Ju-Hyun Lee,George M Church,David R WaltPubMed 34783650
- Cancer cell international 21:4852021Engineered exosomes loaded with miR-449a selectively inhibit the growth of homologous non-small cell lung cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWen Zhou,Mingming Xu,Zhipeng Wang,Mingjun YangPubMed 34521413
- PeerJ 8:e99282020Mesenchymal stem cell exosomes reverse acute lung injury through Nrf-2/ARE and NF-B signaling pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJun Li et. al.PubMed 32999767
- Oncology reports 44:2165-21732020Downregulation of exosomal miR‑1273a increases cisplatin resistance of non‑small cell lung cancer by upregulating the expression of syndecan binding protein.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaolong Zhao et. al.PubMed 32901857
- Oncoimmunology 8:15938052019Circulating exosomes measure responses to therapy in head and neck cancer patients treated with cetuximab, ipilimumab, and IMRT.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarie-Nicole Theodoraki et. al.PubMed 31143513
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.3 - 7.5
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Cell preparation containing CD9 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P21926
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
Integral membrane protein associated with integrins, which regulates different processes, such as sperm-egg fusion, platelet activation and aggregation, and cell adhesion (PubMed:14575715, PubMed:18541721, PubMed:8478605). Present at the cell surface of oocytes and plays a key role in sperm-egg fusion, possibly by organizing multiprotein complexes and the morphology of the membrane required for the fusion (By similarity). In myoblasts, associates with CD81 and PTGFRN and inhibits myotube fusion during muscle regeneration (By similarity). In macrophages, associates with CD81 and beta-1 and beta-2 integrins, and prevents macrophage fusion into multinucleated giant cells specialized in ingesting complement-opsonized large particles (PubMed:12796480). Also prevents the fusion between mononuclear cell progenitors into osteoclasts in charge of bone resorption (By similarity). Acts as a receptor for PSG17 (By similarity). Involved in platelet activation and aggregation (PubMed:18541721). Regulates paranodal junction formation (By similarity). Involved in cell adhesion, cell motility and tumor metastasis (PubMed:7511626, PubMed:8478605).
Alternative names
CD9, MIC3, TSPAN29, GIG2, CD9 antigen, 5H9 antigen, Cell growth-inhibiting gene 2 protein, Leukocyte antigen MIC3, Motility-related protein, Tetraspanin-29, p24, MRP-1, Tspan-29
Recommended products
Mouse Monoclonal CD9 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 12 publications. Immunogen corresponding to Cell preparation containing CD9 protein.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.3 - 7.5
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Cell preparation containing CD9 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P21926
- Carrier free
- Yes
- Clone number
- TS9
- Purity
- Tissue culture supernatant
- Specificity
Recognises the p24 antigen, a 24 kda protein.
- Light chain type
- kappa
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was changed from ascites to tissue culture supernatant on 19th June 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD9 also known as motility-related protein-1 (MRP-1) is a transmembrane glycoprotein with a molecular weight of approximately 24 kDa. This protein is part of the tetraspanin family and shows widespread expression throughout different tissues including hematopoietic and non-hematopoietic cells. CD9 participates in numerous cellular processes by interacting with integrins and other cell surface receptors facilitating the organization of molecular complexes within the cell membrane.
- Biological function summary
The CD9 protein is integral to cell adhesion migration and fusion. It forms complexes with other tetraspanins and proteins like EWI-2 and EWI-F contributing to cellular signaling and membrane compartmentalization. Additionally CD9 plays an important role in the formation and secretion of exosomes tiny vesicles emitted by cells that mediate cell-to-cell communication. These exosomes can be analyzed using tools like exosome assays and detection kits that target CD9 proteins.
- Pathways
CD9 is involved in the regulation of immune response and cell morphology. It participates in pathways such as the integrin signaling pathway and the phosphatidylinositol 3-kinase (PI3K) signaling pathway. CD9 modulates these interactions by associating with proteins such as integrins and other members of the tetraspanin family influencing cellular movement and proliferation.
- Associated diseases and disorders
CD9 has connections to various conditions including cancer and infectious diseases. In cancer CD9 can influence tumor progression and metastasis while its altered expression levels have been associated with different cancer types. CD9 interacts with proteins such as integrins and CD81 in these contexts affecting cellular adhesion and migration mechanisms. In infectious diseases CD9 is involved in viral entry processes as some viruses utilize CD9 and its associated tetraspanins for entry into host cells.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
![Western blot - Anti-CD9 antibody [TS9] - BSA and Azide free (ab58989), expandable thumbnail](https://content.abcam.com/products/images/cd9-antibody-ts9-bsa-and-azide-free-ab58989--western-blot-img99946.jpg "Western blot - Anti-CD9 antibody [TS9] - BSA and Azide free (ab58989)")
Western blot - Anti-CD9 antibody [TS9] - BSA and Azide free (ab58989)
This image was generated using the ascites version of the product.
All lanes: Western blot - Anti-CD9 antibody [TS9] - BSA and Azide free (ab58989)
All lanes: HEL cell lysate at 130000 Cells
Predicted band size: 25 kDa
![Western blot - Anti-CD9 antibody [TS9] - BSA and Azide free (ab58989), expandable thumbnail](https://content.abcam.com/products/images/cd9-antibody-ts9-bsa-and-azide-free-ab58989--western-blot-img13713.jpg "Western blot - Anti-CD9 antibody [TS9] - BSA and Azide free (ab58989)")
Western blot - Anti-CD9 antibody [TS9] - BSA and Azide free (ab58989)
Lanes 1 - 4: Merged signal (red and green). Green - ab58989 observed at 24 kDa. Red - loading control Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) observed at 55 kDa.ab58989 was shown to react with CD9 in wild-type HeLa cells in Western blot with loss of signal observed in CD9 knockout cell line Human CD9 knockout HeLa cell line ab255375 (CD9 knockout cell lysate Human CD9 knockout HeLa cell lysate ab263754). Wild-type HeLa and CD9 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab58989 and Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) overnight at 4 °C at 1 μg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
All lanes: Western blot - Anti-CD9 antibody [TS9] - BSA and Azide free (ab58989) at 1 µg/mL
Lane 1: Wild-type HeLa (No DTT) cell lysate at 20 µg
Lane 2: CD9 knockout HeLa (No DTT) cell lysate at 20 µg
Lane 2: Western blot - Human CD9 knockout HeLa cell line (Human CD9 knockout HeLa cell line ab255375)
Lane 3: A549 (Non-Reducing) cell lysate at 20 µg
Lane 4: MCF7 (Non-Reducing) cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 25 kDa
Observed band size: 24 kDa
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