AB123511

Anti-CD90 / Thy1 antibody [5E10]

Name: Anti-CD90 / Thy1 antibody [5E10] (ab123511) | Abcam
Brand: Abcam Limited
SKU: AB123511
Price: 520 USD
Availability: InStock
Rating: 1 (1 reviews)

(1 Review)

(5 Publications)

Mouse Monoclonal CD90 / Thy1 antibody. Suitable for Flow Cyt and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Cell preparation containing THY1 protein.

View Alternative Names

CD90, Thy-1 membrane glycoprotein, CDw90, Thy-1 antigen, THY1

1 Images

Flow Cytometry - Anti-CD90 / Thy1 antibody [5E10] (AB123511)

Flow Cyt

Unknown

Flow Cytometry - Anti-CD90 / Thy1 antibody [5E10] (AB123511)

Overlay histogram showing Jurkat cells stained with ab123511 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab123511, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 80% methanol (5 min) fixed Jurkat cells used under the same conditions.

Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

5E10

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing THY1 protein. The exact immunogen used to generate this antibody is proprietary information.

P04216

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1-3 µg/mL", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

ab123511 was determined to be > 95% pure by SDS-PAGE.

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD90 also known as Thy1 is a glycoprotein with a molecular mass of approximately 25-37 kDa. It is highly conserved across species and found on the surface of various cell types including fibroblasts neurons and endothelial cells. CD90 is expressed in these cells in tissues such as the brain thymus and lungs. Researchers use anti-CD90 antibodies for detection often in combination with CD90 FITC in immunofluorescence assays. CD90 serves as a useful cell marker particularly in identifying mesenchymal stem cells (MSCs).

Biological function summary

CD90 plays a role in cell-cell and cell-matrix interactions contributing to cellular adhesion migration and signal transduction. It functions as part of a complex involving integrins which mediate its interaction with the extracellular matrix. This protein also influences T-cell activation reflecting its importance in immune response regulation. Its presence aids in modulating response to injury and promoting tissue repair due to its association with stem and progenitor cells.

Pathways

CD90 engagement affects focal adhesion and MAPK signaling pathways. It interacts with integrins like αvβ3 and α5β1 influencing cell movement and proliferation processes. CD90 involvement in these pathways allows it to regulate responses important for cell survival and communication within various tissues. These interactions highlight the protein's role in maintaining cellular homeostasis and regulating physiological processes.

CD90 has associations with fibrosis and cancer. In fibrotic conditions CD90 expression affects fibroblast activity influencing tissue scarring in organs such as the liver and lungs. In cancer CD90 modifies tumor progression and metastasis by impacting cell adhesion and migration with connections to proteins like TGF-beta which further drive these processes. Understanding CD90 in disease contexts facilitates the development of therapeutic strategies aimed at modulating its activity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.

See full target information THY1

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Bioengineering (Basel, Switzerland) 11: PubMed38927821

2024

Development of Subcutaneous SSEA3- or SSEA4-Positive Cell Capture Device.

Applications

Unspecified application

Species

Unspecified reactive species

Yasuhide Nakayama,Ryosuke Iwai

Journal of tissue engineering and regenerative medicine 16:311-329 PubMed34962086

2022

LncRNA metastasis-associated lung adenocarcinoma transcript-1 promotes osteogenic differentiation of bone marrow stem cells and inhibits osteoclastic differentiation of Mø in osteoporosis via the miR-124-3p/IGF2BP1/Wnt/β-catenin axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangxin Li

Cell cycle (Georgetown, Tex.) 20:1953-1966 PubMed34455928

2021

Neohesperidin promotes the osteogenic differentiation of human bone marrow stromal cells by inhibiting the histone modifications of lncRNA SNHG1.

Applications

Unspecified application

Species

Unspecified reactive species

Chuanxin Zhang,Shuai Yuan,Yi Chen,Bo Wang

Chinese medical journal 131:2089-2096 PubMed30127219

2018

Effect of Optimized Concentrations of Basic Fibroblast Growth Factor and Epidermal Growth Factor on Proliferation of Fibroblasts and Expression of Collagen: Related to Pelvic Floor Tissue Regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan-Yuan Jia,Jing-Yi Zhou,Yue Chang,Fang An,Xiao-Wei Li,Xiao-Yue Xu,Xiu-Li Sun,Chun-Yang Xiong,Jian-Liu Wang

Molecular medicine reports 17:7902-7910 PubMed29620288

2018

Differentiation of genetically modified canine bone mesenchymal stem cells labeled with superparamagnetic iron oxide into neural‑like cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xing-Long Liu,Qing-Quan Zu,Bin Wang,Shan-Shan Lu,Xiao-Quan Xu,Sheng Liu,Hai-Bin Shi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD90 / Thy1 antibody [5E10]

Flow Cytometry - Anti-CD90 / Thy1 antibody [5E10] (AB123511)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

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